1887

Abstract

SUMMARY

The study of human cytomegalovirus (HCMV) in cultures of human embryo lung fibroblasts, pre-treated with actinomycin D, has shown that under these conditions the virus infection does not proceed beyond the ‘early’ events of the virus replication cycle.

In the same experimental conditions the growth of poliovirus type 1, vaccinia virus and herpes simplex type 1 virus, was completely unaffected.

These results suggest that the complete HCMV replication cycle requires some cellular function(s) between early transcription of the input virus genome and virus DNA synthesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-42-2-423
1979-02-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/42/2/JV0420020423.html?itemId=/content/journal/jgv/10.1099/0022-1317-42-2-423&mimeType=html&fmt=ahah

References

  1. Benyesh-Melnick M. 1969; Cytomegaloviruses. In Diagnostic Procedures for Viral and Rickettsial Infections pp 701–732 Edited by Lennette E. H., Schmidt N. J. New York: American Public Health Association;
    [Google Scholar]
  2. Ben-Zeev A., Becker Y. 1977; Requirement of host cell RNA polymerase II in the replication of herpes simplex virus in a-amanitin-sensitive and resistant cell lines. Virology 76:246–253
    [Google Scholar]
  3. Costanzo F., Campadelli-Fiume G., Foa’-Tomasi L., Cassai E. 1977; Evidence that herpes simplex virus DNA is transcribed by cellular RNA polymerase B. Journal of Virology 76:996–1001
    [Google Scholar]
  4. Demarchi J. M., Kaplan A. S. 1976; Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis. Journal of Virology 18:1063–1070
    [Google Scholar]
  5. Demarchi J. M., Kaplan A. S. 1977; Physiological state of human embryonic lung cells effects their response to human cytomegalovirus. Journal of Virology 23:126–132
    [Google Scholar]
  6. Estes J. E., Huang E. S. 1977; Stimulation of cellular thymidine kinase by human cytomegalovirus. Journal of Virology 24:13–21
    [Google Scholar]
  7. Furukawa T., Fioretti A., Plotkin S. A. 1973; Growth characteristic of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology 11:991–997
    [Google Scholar]
  8. Furukawa T., Tanaka S., Plotkin S. A. 1975; Inhibition of human cytomegalovirus by rifampin. Journal of General Virology 28:355–362
    [Google Scholar]
  9. Geder L. 1976; Evidence for early nuclear antigens in cytomegalovirus-infected cells. Journal of General Virology 32:315–319
    [Google Scholar]
  10. Huang E. S. 1975; Human cytomegalovirus III. Virus induced DNA polymerase. Journal of Virology 16:298–310
    [Google Scholar]
  11. Lancini G., Zanichelli W. 1977; Structure-activity relationships in rifamycins. In Structure-activity Relationships Among the Semisynthetic Antibiotics pp 531–600 Edited by Perlman D. London: Academic Press;
    [Google Scholar]
  12. Laschi R., Rizzoli C. 1968; Etude ultrastructurale de cultures primaires de foie embryonnaire. Mise au point des techniques et premières observations. Journal de Microscopie 7:533–538
    [Google Scholar]
  13. Michelson-Fiske S., Horodniceanu F., Guillon J. C. 1977; Immediate early antigens in human cytomegalovirus infected cells. Nature 270:615–617
    [Google Scholar]
  14. Plummer G., Goodheart C. R., Henson D., Blowling C. P. 1969; A comparative study of the DNA density and behaviour in tissue cultures of fourteen different herpes viruses. Virology 39:134–137
    [Google Scholar]
  15. Rapp F., Rasmussen L. E., Benyesh-Melnick M. 1963; The immunofluorescent focus technique in studying the replication of cytomegalovirus. Journal of Immunology 91:709–719
    [Google Scholar]
  16. Reed J. L., Muench M. 1938; A simple method of estimating fifty percent end points. American Journal of Hygiene 27:493–497
    [Google Scholar]
  17. Riva S., Fietta A., Silvestri L. G. 1972; Mechanism of action of a rifamycin derivative (AF/013) which is active on the nucleic acid polymerases insensitive to rifampicin. Biochemical and Biophysical Research Communications 49:1263–1271
    [Google Scholar]
  18. St Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. 1974; Stimulation of cellular DNA synthesis by human cytomegalovirus. Journal of Virology 13:353–362
    [Google Scholar]
  19. St Jeor S. C., Hutt R. 1977; Cell DNA replication as a function in the synthesis of human cytomegalovirus. Journal of General Virology 37:65–73
    [Google Scholar]
  20. Tanaka S., Furukawa T., Plotkin S. A. 1975; Human cytomegalovirus stimulates host cell RNA synthesis. Journal of Virology 15:297–304
    [Google Scholar]
  21. The T. H., Klein G., Langenhuysen M. M. A. C. 1974; Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection. Clinical and Experimental Immunology 16:1–12
    [Google Scholar]
  22. Wentworth B. B., French L. 1970; Plaque assay of cytomegalovirus strains of human origin. Proceedings of the Society for Experimental Biology and Medicine 135:253–258
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-42-2-423
Loading
/content/journal/jgv/10.1099/0022-1317-42-2-423
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error