1887

Abstract

SUMMARY

The intracellular ATP content of ATCC 27092 grown in a glucose-containing medium was almost constant (2 to 3 µg/mg dry wt. cells) through the early to middle stage of logarithmic phase, but it was lowered to less than 0.1 µg/mg after cessation of growth owing to the exhaustion of available glucose. All the cells in the early stage of stationary phase were still viable and thus considered to be in a starved state. When such starved cells were infected with PL-1 phages in a tris-maleate buffer of pH 6.0, the process of forming blender-resistant phage-cell complexes signifying the complete infection of phage genomes into the cells was much inhibited. There was a good correlation between the ATP content of cells and the extent of the formation of blender-resistant phage-cell complexes and the correlation coefficient between them was 0.89 ± 0.09 at the 95% confidence limit. On the other hand, the process of forming both the phage-adsorbed cells and the anti-phage serum-resistant phage-cell complexes were not affected by the ATP content of cells. Feeding of glucose to such starved cell cultures caused the cells to restore both the ATP content and the ability to form blender-resistant phage-cell complexes. Such restoration was also observed when the starved cells collected by centrifugation were incubated in a glucose-containing medium.

The significance of the intracellular level of high energy compounds such as ATP for the mechanism of the injection of phage genomes into the cells is discussed.

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1979-01-01
2022-01-16
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References

  1. Ackerman H. W., Eisenstark A. 1974; The present state of phage taxonomy. Intervirology 3:201–219
    [Google Scholar]
  2. Cox G. B., Gibson F. 1974; Studies on electron transport and energy-linked reactions using mutants of Escherichia coli. Biochimica et Biophysica Acta 346:1–25
    [Google Scholar]
  3. Denhardt D. T., Sinsheimer R. L. 1965; The process of infection with bacteriophage ϕ X174. III. Phage maturation and lysis after synchronized infection. Journal of Molecular Biology 12:641–646
    [Google Scholar]
  4. Hancock R. E. W., Braun V. 1976; Nature of the energy requirement for the irreversible adsorption of bacteriophage T1 and ϕ 80 to Escherichia coli. Journal of Bacteriology 125:409–415
    [Google Scholar]
  5. Hayes W. 1963; The bacteriophage model. In Mechanism of Virus Infection pp 36–99 Edited by Wilson Smith. London and New York: Academic Press;
    [Google Scholar]
  6. Hershey A. D., Chase M. 1952; Independent function of viral protein and nucleic acid in growth of bacteriophage. Journal of General Physiology 36:39–56
    [Google Scholar]
  7. Jetten A. M., Jetten M. E. R. 1975; Energy requirement for the initiation of colicin action in Escherichia coli. Biochimica et Biophysica Acta 387:12–22
    [Google Scholar]
  8. Kaback H. R. 1972; Transport across isolated bacterial cytoplasmic membrane. Biochimica et Biophysica Acta 265:367–416
    [Google Scholar]
  9. Kleine W. L., Boyer P. D. 1972; Energization of active transport by Escherichia coli. Journal of Biological Chemistry 247:7257–7265
    [Google Scholar]
  10. Kozloff L. M., Lute M. 1959; A contractile protein in the tail of bacteriophage T2. Journal of Biological Chemistry 234:539–546
    [Google Scholar]
  11. Kozloff L. M., Lute M., Hederson K. 1957; Viral invasion. I. Rupture of thiol ester bonds in the bacteriophage tail. Journal of Biological Chemistry 228:511–528
    [Google Scholar]
  12. Lanni Y. T. 1965; DNA transfer from phage T5 to host cells: dependence on intercurrent protein synthesis. Proceedings of the National Academy of Sciences of the United States of America 53:969–973
    [Google Scholar]
  13. Lanni Y. T. 1969; Functions of two genes in the first-step-transfer DNA of bacteriophage T5. Journal of Molecular Biology 44:173–183
    [Google Scholar]
  14. Lanni F., Lanni Y. T. 1966; Genetic suppressors of bacteriophage T5 amber mutants. Journal of Bacteriology 92:521–523
    [Google Scholar]
  15. Lanni Y. T., Lanni F., Tevethia M. J. 1966; Bacteriophage T5 chromosome fractionation: genetic specificity of a DNA fragment. Science 152:208–210
    [Google Scholar]
  16. Lanni Y. T., McCorquodale D. J., Wilson C. M. 1964; Molecular aspects of DNA transfer from phage T5 to host cells. (II) Origin of first-step-transfer DNA fragments. Journal of Molecular Biology 10:19–27
    [Google Scholar]
  17. Lovett P. S., Shockman G. D. 1970; Characteristics of bacteriophage N1 and its attachment to cells of Micrococcus lysodeikticus. Journal of Virology 6:125–134
    [Google Scholar]
  18. McCorquodale D. J., Lanni Y. T. 1964; Molecular aspects of DNA transfer from phage T5 to host cells. (I) Characterization of first-step-transfer materials. Journal of Molecular Biology 10:10–18
    [Google Scholar]
  19. McCorquodale D. J., Buchanan J. M. 1968; Patterns of protein synthesis in T5-infected Escherichia coli. Journal of Biological Chemistry 243:2550–2559
    [Google Scholar]
  20. Ore A., Pollard E. 1956; Physical mechanism of bacteriophage injection. Science 124:430–432
    [Google Scholar]
  21. Simon L. D., Anderson T. F. 1967; The infection of Escherichia coli by T2 and T4 bacteriophage as seen in the electron microscope. Virology 32:279–297
    [Google Scholar]
  22. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–23
    [Google Scholar]
  23. Strange R. E., Wade H. E., Dark F. A. 1963; Effect of starvation of adenosine triphosphate concentration in Aerobacter aerogenes. Nature, London 199:55–57
    [Google Scholar]
  24. Strehler B. L., Trotter J. R. 1954; Determination of ATP and related compounds: firefly luminescence and other methods. In Methods of Biochemical Analysis vol 1 pp 341–356 Edited by David Glick. New York: Interscience Publishers Inc;
    [Google Scholar]
  25. Tanami Y. 1963; Mechanism of virus infection. Processes in bacteriophage infection and their genetic control. (In Japanese.) Tanpakushitsu Kakusan Kohso 8:790–798
    [Google Scholar]
  26. Watanabe K., Takesue S. 1973; Energy-requirement for the formation of blender-resistant complexes in Lactobacillus phage infection. Journal of General Virology 20:319–326
    [Google Scholar]
  27. Zarybnicky V. 1969; Mechanism of T-even DNA ejection. Journal of Theoretical Biology 22:33–42
    [Google Scholar]
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