The conditions for optimal transduction efficiency of the phage SPP1 have been investigated. By irradiating transducing lysates with u.v. light we have been able to obtain a fivefold increase in the number of transductants and to reduce strongly the interference caused by infective particles. Any dependence of SPP1 transduction on PBSX induction has been ruled out by the use of mutants, which are unable to induce the defective phage. SPP1 mediated transduction is susceptible to the restriction and modification system of . The functions involved in the recombination of the SPP1 transduced DNA fragment are probably identical to those required in DNA transformation and heterologous PBS1 transduction.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error