1887

Abstract

SUMMARY

Particles of the Puerto Rico isolate of cowpea mosaic virus (CPMV-PR), which belongs in the severe subgroup, had one electrophoretic form when analysed by 2·5% polyacrylamide gel electrophoresis and zone electrophoresis in sucrose gradients at pH 7·8, 7·5, and 5·5. The electrophoretic properties of CPMV-PR did not vary with time after inoculation but the virus could be converted to a slower migrating form by treatment with trypsin. The partial conversion by trypsin was accompanied by and was possibly the result of proteolytic conversion of the S protein subunit to a lower molecular mass form. Analysis of proteins of untreated CPMV-PR suggested that the S subunit of the virus could also be converted by plant proteases to a smaller form without any change in the particle net surface charge, while treatment with trypsin affected those S subunits not previously converted by plant proteases, by converting them to a form slightly larger and with a greater positive charge than the S subunit produced by action of the plant proteases. This hypothesis accounts both for the decrease in electrophoretic mobility and for the fact that the conversion was only partial.

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1978-10-01
2022-08-14
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References

  1. Agrawal H. O. 1964; Identification of cowpea mosaic virus isolates. Mededelingen Landbouwhogeschool, Wageningen 64:51–53
    [Google Scholar]
  2. Geelen J. L. M. C., Van Kammen A., Verduin B. J. M. 1972; Structure of the capsid of cowpea mosaic virus. The chemical subunit : molecular weight and number of subunits per particle. Virology 49:205–213
    [Google Scholar]
  3. Geelen J. L. M. C., Rezelman G., Van Kammen A. 1973; The infectivity of the two electrophoretic forms of cowpea mosaic virus. Virology 51:279–286
    [Google Scholar]
  4. Gillaspie A. G., Bancroft I. B. 1965; The rate of accumulation, specific infectivity, and electrophoretic characteristics of bean pod mottle virus in bean and soybean. Phytopathology 55:906–908
    [Google Scholar]
  5. Knowland J. 1974; Protein synthesis directed by the RNA from a plant virus in a normal animal cell. Genetics 78:383–394
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  7. Lee R. F., Johnson L. B., Niblett C. L. 1975; Effect of host enzyme extracts on the electrophoretic forms and specific infectivity of cowpea mosaic virus. Physiological Plant Pathology 7:59–69
    [Google Scholar]
  8. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  9. Mclaughlin M. R., Thongmeearkom P., Milbrath G. M., Goodman R. M. 1977; Isolation and some properties of a yellow subgroup member of cowpea mosaic virus from Illinois. Phytopathology 67:844–847
    [Google Scholar]
  10. Niblett C. L., Semancik J. S. 1969; Conversion of the electrophoretic forms of cowpea mosaic virus in vivo and in vitro . Virology 38:685–693
    [Google Scholar]
  11. Peacock A. C., Dingman C. W. 1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  12. Shepherd R. J. 1963; Serological relationship between bean pod mottle virus and cowpea mosaicviruses from Arkansas and Trinidad. Phytopathology 53:865–866
    [Google Scholar]
  13. Spencer P. W., Titus J. S., Spencer R. D. 1975; Direct fluorimetrie assay for proteolytic activity against intact proteins. Analytical Biochemistry 64:556–566
    [Google Scholar]
  14. Thongmeearkom P., Goodman R. M. 1976; A severe disease of soybeans caused by an isolate of cowpea mosaic virus. Proceedings of the American Phytopathological Society 3:209–210 abstract
    [Google Scholar]
  15. Thongmeearkom P., Goodman R. M. 1978; Complementation and pseudorecombination between ribonucleic acids from two natural isolates of cowpea mosaic virus (severe subgroup). Virology 85:75–83
    [Google Scholar]
  16. Weber G., Young L. B. 1964; Fragmentation of bovine serum albumin by pepsin. I. The origin of the acid expansion of the albumin molecule. Journal of Biological Chemistry 239:1415–1430
    [Google Scholar]
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