1887

Abstract

SUMMARY

multiplication of rabies virus was inhibited by a condensed mineral ion, ammonium-5-tungsto-2-antimoniate (HPA 23). The inhibitory effect was evaluated by two different methods, plaque reduction and one step virus growth. Plaquing showed 50% inhibition with 4.5 g/ml of HPA 23 and complete inhibition with 12.5 g/ml. A reduction of two logs in virus yield was obtained in BHKCS cells in suspension treated with 50 g/ml of HPA 23. Inhibition also occurred when treatment with HPA 23 was started 18 to 24 h after infection in the plaque assay but no effect was seen when HPA 23 was added 48 h after virus inoculation. All these inhibitory effects of HPA 23 on rabies virus multiplication were observed at non cytotoxic doses. Therefore HPA 23 contrasts with other antiviral drugs which do not inhibit rabies virus multiplication without affecting the viability of cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-3-665
1978-09-01
2022-09-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/3/JV0400030665.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-3-665&mimeType=html&fmt=ahah

References

  1. Ablashi D. V., Twardzik D. R., Easton J. M., Armstrong G. R., Luetzeler J., Jasmin C., Chermann J. C. 1977; Effects of 5-tungsto-2-antimoniate in oncogenic DNA and RNA virus-cell systems. European Journal of Cancer 13:713–720
    [Google Scholar]
  2. Chermann J. C., Sinoussi F. C., Jasmin C. 1975; Inhibition of RNA dependant DNA-polymerase of murine oncornaviruses by ammonium-5-tungsto-2-antimoniate. Biochemical and. Biophysical Research Communications 65:1229–1236
    [Google Scholar]
  3. Flamand A., Pese D., Bussereau F. 1977; Effect of actinomycin D and cytosine arabinoside on rabies and VSV multiplication. Virology 78:323–327
    [Google Scholar]
  4. Hamparian V. V., Hilleman M. R., Ketler A. 1963; Contributions to characterization and classification of animal viruses. Proceedings of the Society for Experimental Biology and Medicine 112:1040–1050
    [Google Scholar]
  5. Harmon M. W., Janis B. 1976; Effects of cytosine arabinoside, adenine arabinoside and 6-azauridine on rabies virus in vitro and in vivo . Journal of Infectious Diseases 133:7–13
    [Google Scholar]
  6. Jasmin C., Chermann J. C., Herve G., Souchay P., Boy-Lousteau C., Raybaud N., Sinuoussi F. C., Raynaud M. 1974; In vivo inhibition of murine leukemia and sarcoma viruses by the heteropolyanion 5-tungsto-2-antimoniate. Journal of the National Cancer Institute 53:469–474
    [Google Scholar]
  7. Jasmin C., Chermann J. C., Raynaud M., Werner G. H., Raybaud N., Sinoussi F. C., Boy-Lousteau C. 1975; In vivo and in vitro antiviral activity of the mineral condensed heteropolyanion 5-tungsto-2-antimoniate. Proceedings of the 8th International Congress of Chemotherapy, Athens vol 2:956–962
    [Google Scholar]
  8. Raybaud N., Jasmin C., Huppert J., Chermann J. C., Mathe G., Raynaud M. 1972; Study Of antivirus activity of mineral condensed polyanions. II. Effect on vesicular stomatitis virus. European Journal of Clinical and Biological Research 17:295–299
    [Google Scholar]
  9. Raynaud M., Chermann J. C., Plata F., Jasmin C., Mathe G. 1971; Inhibiteurs des virus du groupe leucémie-sarcomes murins. Silico-tungstate. Comptes Rendus hebdomedaire des seances de l’ Académie des Sciences, Paris 272:347–348
    [Google Scholar]
  10. Sedwick W. D., Wiktor T. J. 1967; Reproducible plaquing system for rabies, LCM and other RNA viruses in BHK 21/13 S agarose suspensions. Journal of Virology 1:1224–1226
    [Google Scholar]
  11. Streissle G. 1975; The effect of antiviral compounds on acute and persistent LMC virus infections. 3rd International Congress for Virology Madrid 10–17 September, 1975. Abstract C 308 253
    [Google Scholar]
  12. Tsiang H., Atanasiu P. 1971; Replication du virus rabique fixe en suspension cellulaire. Comptes Rendus hebdomadaire des seances de l’ Académie des Sciences, Paris série D 272:897–900
    [Google Scholar]
  13. Werner G. H., Jasmin C., Chermann J. C. 1976; Effect of ammonium 5-tungsto-2-antimoniate on encephalomyocarditis and vesicular stomatitis virus infections in mice. Journal of General Virology 31:59–64
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-3-665
Loading
/content/journal/jgv/10.1099/0022-1317-40-3-665
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error