1887

Abstract

SUMMARY

By DNA-DNA reassociation kinetic analysis, less than one genome equivalent per cell of human CMV-DNA was found in two lymphoblastoid cell lines, one derived from the peripheral blood of a congenitally infected male infant at the age of 21 months (D4 cell line), the other obtained by co-cultivation of lethally X-irradiated cells from the 9-month lymphoblastoid cell line previously described by Joncas ., (1975) with cord blood leukocytes of a female newborn (M1 cell line). Human CMV antigens could not be detected and virus could not be rescued from these cells by co-cultivation with fully permissive human fibroblasts. It may be that the CMV-DNA is defective. Epstein–Barr virus DNA as well as EBNA and EBV-EA antigens were present in these cell lines. Both lines express surface markers characteristic of thymus-independent, B lymphocytes.

The CMV-DNA of the CMV-DU strain, isolated from this infant’s urine five times successively from the age of 1 day to 30 months, appears to be closely related to the DNA of the AD-169 strain by reciprocal hybridization and by electrophoretic pattern analysis of the restriction enzyme cleavage products. Experimental attempts to transform cord blood leukocytes with this urine strain of CMV before or after u.v. irradiation have so far failed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-3-519
1978-09-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/3/JV0400030519.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-3-519&mimeType=html&fmt=ahah

References

  1. Adams A. 1975; Preparation of Epstein–Barr virus from P3HR-1 cells and isolation of the virus DNA. Epstein–Barr virus production concentration and purification. IARC Report 75003:129–146
    [Google Scholar]
  2. Baringer J. R. 1974; Recovery of herpes-simplex virus from human sacral ganglions. New England Journal of Medicine 291:828–830
    [Google Scholar]
  3. Baringer J. R., Swoveland P. 1973; Recovery of herpes-simplex virus from human trigeminal ganglions. New England Journal of Medicine 288:648
    [Google Scholar]
  4. Bastian F. O., Rabson A. S., Yee C. L., Tralka T. S. 1972; Herpesvirus hominis: isolation from human trigeminal ganglion. Science 178:306–307
    [Google Scholar]
  5. Carter R. L., Penman H. G. 1969 In Infectious Mononucleosis pp 211–212 Oxford and Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  6. Chagnon A., Pavilanis V. 1965; L’établissement de souches cellulaires diploides humaines in vitro . La Revue Canadienne de Biologie 24:35–43
    [Google Scholar]
  7. De Thé T. H., Klein G., Langenhuysen M. M. A. C. 1974; Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection. Clinical and Experimental Immunology 16:1–12
    [Google Scholar]
  8. Geder L. 1976; Evidence for early nuclear antigens in cytomegalovirus-infected cells. Journal of General Virology 32:315–319
    [Google Scholar]
  9. Greene P. J., Betlach M. C., Goodman H. M., Boyer H. W. 1974; The Eco R-1 restriction endonuclease. Methods in Molecular Biology 7:87–105
    [Google Scholar]
  10. Hanshaw J. E., Niederman J. C., Chessin L. N. 1972; Cytomegalovirus macroglobulin in cell-associated herpesvirus infections. Journal of Infectious Diseases 125:304–306
    [Google Scholar]
  11. Huang E.-S., Chen S. T., Pagano J. S. 1973; Human cytomegalovirus. I. Purification and characterization of viral DNA. Journal of Virology 12:1473–1481
    [Google Scholar]
  12. Huang R.-S., Pagano J. S. 1975; Replication of cytomegalovirus DNA in human lymphoblastoid cells. Oncogenesis and Herpesviruses 11:475–481 (IARC Scientific Publication no. 11)
    [Google Scholar]
  13. Joncas J. 1976; Laboratory diagnosis of infectious mononucleosis: Epstein–Barr virus and the heterophil test. Proceedings of an ASM symposium on Modem Methods in Medical Microbiology chapter 10, pp 105–125 University Park Press;
    [Google Scholar]
  14. Joncas J., Chicoine L., Thivierge R., Bertrand M. 1974; Epstein–Barr virus antibodies in the cerebrospinal fluid. American Journal of Diseases 127:282–285
    [Google Scholar]
  15. Joncas J., Menezes J., Huang E.-S. 1975; Persistence of CMV genome in lymphoid cells after congenital infection. Nature, London 258:432–434
    [Google Scholar]
  16. Joncas J., Wills A., McLaughlin B. 1977; Congenital infection with cytomegalovirus and Epstein–Barr virus: case report. Canadian Medical Association Journal 117:1417–1418
    [Google Scholar]
  17. Jondal M., Klein G. 1973; Surface markers on human B and T lymphocytes. II. Presence of Epstein–Barr virus receptors on B lymphocytes. Journal of Experimental Medicine 138:1365–1378
    [Google Scholar]
  18. Jovin T. M., Englund P. T., Bertsch L. L. 1969; Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. Journal of Biological Chemistry 244:2996–3008
    [Google Scholar]
  19. Keller R., Peitchel R., Goldman J. N., Goldman M. 1976; An IgG-Fc receptor induced in cytomegalovirus-infected human fibroblasts. Journal of Immunology 116:772–777
    [Google Scholar]
  20. Kilpatrick B. A., HuAng E.-S., Pagano J. S. 1976; Analysis of cytomegalovirus genomes with restriction endonucleases Hin D III and Eco R-1. Journal of Virology 18:1095–1105
    [Google Scholar]
  21. Kohne P. E., Britten J. 1971; Hydroxyapatite technique for nucleic acid reassociation. Progress in Nucleic Acid Research 2:500–507
    [Google Scholar]
  22. Lennette E. H., Schmidt N. J. 1969 Diagnostic Procedures for Viral and Rickettsial Infections 4th edition, pp 723–732 American Public Health Association;
    [Google Scholar]
  23. Lussier G., Chagnon A., Pavilanis V. 1966; Etude du caryotype d’une souche cellulaire humaine foetale. La Revue Canadienne de Biologie 25:57–61
    [Google Scholar]
  24. Menezes J., Leibold W., Klein G., Clements G. 1975; Establishment and characterization of an Epstein–Barr virus (EBV) negative lymphoblastoid B cell line (BJA-B) from an exceptional, EBV-genome-negative African Burkitt’s lymphoma. Biomedicine 22:276–284
    [Google Scholar]
  25. Menezes J., Jondal M., Leibold W., Dorval G. 1976; Epstein–Barr virus interactions with human lymphocyte subpopulations: virus adsorption, kinetics of expression of Epstein–Barr virus-association nuclear antigen, and lymphocyte transformation. Infection and Immunity 13:303–310
    [Google Scholar]
  26. Menezes J., Seigneurin J. M., Patel P., Bourkas A., Lenoir G. 1977; Presence of Epstein–Barr virus receptors, but absence of virus penetration, in cells of an Epstein–Barr virus genome-negative human lymphoblastoid T line (Molt 4). Journal of Virology 22:816–821
    [Google Scholar]
  27. Minowada J., Ohnuma T., Moore G. E. 1972; Rosette forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus derived lymphocytes. Journal of the National Cancer Institute 49:891–895
    [Google Scholar]
  28. Nilsson K. 1971; High-frequency establishment of human immunoglobulin-producing lymphoblastoid lines from normal and malignant lymphoid tissue and peripheral blood. International Journal of Cancer 8:432–442
    [Google Scholar]
  29. Nonoyama M., Pagano J. S. 1973; Homology between Epstein–Barr virus DNA and viral DNA from Burkitt’s lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics. Nature, London 242:44–47
    [Google Scholar]
  30. Rapp F., Geder L., Murasko D., Lausch R., Ladda R., Huang E.-S., Webber M. M. 1976; Long-term persistence of cytomegalovirus genome in cultured human cells of prostatic origin. Journal of Virology 18:982–990
    [Google Scholar]
  31. Richardson C. C., Schildkraut C. L., Aposhiand H. V., Kornberg A. 1964; Enzymatic Synthesis of deoxyribonucleic acid. XIV. Further purification and properties of deoxyribonucleic acid polymerase of Escherichia coli . Journal of Biological Chemistry 239:222–232
    [Google Scholar]
  32. Smith H. O., Wilcox K. W. 1970; A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. Journal of Molecular Biology 51:379–391
    [Google Scholar]
  33. St. Jeor S., Weisser A. 1977; Persistence of cytomegalovirus in human lymphoblasts and peripheral leukocyte cultures. Infection and Immunity 15:402–409
    [Google Scholar]
  34. Westmoreland D., Watkins J. F. 1974; The IgG receptor induced by herpes simplex virus: studies using radioiodinated IgG. Journal of General Virology 24:167–178
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-3-519
Loading
/content/journal/jgv/10.1099/0022-1317-40-3-519
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error