1887

Abstract

SUMMARY

A non-specific reaction between human IgM and cytoplasmic structures of virus infected cells can often be observed if IgM antibodies to virus antigens are detected by indirect immunofluorescence or by immuno enzyme assays. Formaldehyde selectively inactivates the cytoplasmic receptors for human IgM without affecting the virus structural proteins. Alternatively, receptor-free antigens can be obtained by isolation of nuclei from virus infected cells. Due to reduced back-ground, a more specific and more sensitive detection of IgM antibodies to Epstein-Barr virus, cytomegalovirus or central European encephalitis virus is possible.

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1978-08-01
2024-12-01
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References

  1. Fraser K. B., Shirodaria P. V., Stanford F. 1971; Fluorescent staining and human IgM. British Medical Journal 3:707
    [Google Scholar]
  2. Goldwasser R. A., Rozansky R. 1958; Staining of complement and modification of fluorescent antibody procedures. Journal of Immunology 80:122–131
    [Google Scholar]
  3. Keller R., Peitscher R., Goldman J. N., Goldman M. 1976; An IgG-Fc receptor induced in cytomegalovirus-infected human fibroblasts. Journal of Immunology 116:772–777
    [Google Scholar]
  4. Knez V., Stewart J. A., Ziegler D. 1976; Cytomegalovirus specific IgM and IgG responses in humans studied by radioimmunoassay. Journal of Immunology 117:2006–2013
    [Google Scholar]
  5. Reimer C. B., Black C. M., Phillips R. J., Logan L. C., Hunter E. F., Pender B. J., Mcgrew B. E. 1975; The specificity of fetal IgM. Antibody or anti-antibody?. Annals of the New York Academy of Sciences 254:77–91
    [Google Scholar]
  6. Ross C. E., Cochran A. J., Hoyle D. E., Grant M. R., Mackie R. M. 1973; Formalin-fixed tumor cells in the leucocyte migration test. Lancet ii:1087–1088
    [Google Scholar]
  7. Schmitz H., Haas R. 1972; Determination of different cytomegalovirus immunoglobulins by immunofluorescence. Archiv für die gesamte Virusforschung 37:131–140
    [Google Scholar]
  8. Schmitz H., Scherer M. 1972; IgM antibodies to Epstein-Barr virus in infections mononucleosis. Archiv für die gesamte Virusforschung 37:332–339
    [Google Scholar]
  9. Schmitz H., Doerr H. W., Kampa D., Vogt A. 1977; Solid-phase enzyme-immunoassay for IgM antibodies to cytomegalovirus. Journal of Clincal Microbiology 5:629–634
    [Google Scholar]
  10. Westmoreland D., St. Jeor S., Rapp F. 1976; The development by cytomegalovirus-infected cells of binding activity for normal human immunoglobulin. Journal of Immunology 116:1566–1570
    [Google Scholar]
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