1887

Abstract

SUMMARY

Circular dichroism studies (CD) of turnip yellow mosaic virus (TYMV) nucleoprotein and of its isolated RNA and capsid revealed that: (i) the nucleic acid structure, which comprises a considerable amount of base pairing and/or stacking, remains essentially unchanged irrespective of whether the RNA is encapsidated or free; (ii) the secondary structure of the protein component is mainly accounted for by β- and irregular forms without appreciable amounts of α-helix; (iii) the interaction of capsid protein and RNA induces some conformational changes in the protein probably involving a decrease of β-structure and a perturbation of the microenvironment of some aromatic residues. The influence of temperature on the CD spectra of virus nucleoprotein, RNA and capsid was also investigated. The results are discussed in connection with particle stability.

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1978-08-01
2022-07-02
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References

  1. Brahms J., Michelson A. M., Van Holde K. E. 1966; Adenylate oligomers in single- and double-strand conformation. Journal of Molecular Biology 15:467–488
    [Google Scholar]
  2. Budzynski A. Z. 1971; Circular dichroism studies on coat protein of some strains and mutants of tobacco mosaic virus. Biochimica et Biophysica Acta 251:292–302
    [Google Scholar]
  3. Dunn D. B., Hitchborn J. H. 1965; The use of bentonite in the purification of plant viruses. Virology 25:111–192
    [Google Scholar]
  4. Fasman G. D., Lindblow G., Grossman L. 1964; The helical conformations of polycytidylic acid: studies on the forces involved. Biochemistry 3:1015–1021
    [Google Scholar]
  5. Finch J. T., Klug A. 1966; Arrangement of protein subunits and the distribution of nucleic acid in turnip yellow mosaic virus. II. Electron microscope studies. Journal of Molecular Biology 15:344–364
    [Google Scholar]
  6. Gratzer W. B., Richards E. G. 1971; Evaluation of RNA conformation from circular dichroism and optical rotatory dispersion data. Biopolymers 10:2607–2614
    [Google Scholar]
  7. Hartman K. A., Clayton N., Thomas G. L. 1973; Studies of virus structure by Raman spectroscopy. I. R17 virus and R17 RNA. Biochemical and Biophysical Research Communications 50:942–949
    [Google Scholar]
  8. Horwitz J., Strickland E. H., Billups C. 1969; Analysis of vibrational structure in the near-ultraviolet circular dichroism and absorption spectra of phenylalanine and its derivatives. Journal of the American Chemical Society 91:184–190
    [Google Scholar]
  9. Isenberg H., Cotter R. I., Gratzer W. B. 1971; Secondary structure and interaction of RNA and protein in a bacteriophage. Biochimica et Biophysica Acta 232:184–191
    [Google Scholar]
  10. Jacrot B., Chauvin C., Witz J. 1977; Comparative neutron small-angle scattering study of small spherical RNA viruses. Nature, London 266:417–421
    [Google Scholar]
  11. Jirgensons B. 1976; Conformational transitions of monellin, an intensely sweet protein. Biochimica et Biophysica Acta 446:255–261
    [Google Scholar]
  12. Jonard G. 1972; Contribution a l’etude des interactions RNA–proteine et protenine–proteine dans le virus de la mosaique jaune du navet. Ph.D. thesis University of Strasbourg;
    [Google Scholar]
  13. Kaper J. M. 1971; Studies on the stabilizing forces of simple RNA viruses. I. Selective interference with protein–RNA interactions in turnip yellow mosaic virus. Journal of Molecular Biology 56:259–276
    [Google Scholar]
  14. Kaper J. M. 1972; Experimental analysis of the stabilizing interactions of simple RNA viruses. In RNA Viruses: Replication and Structure Edited by Jaspars E. M. J., Van Kammen A. Federation of European Biochemical Societies vol 27: pp 19–41
    [Google Scholar]
  15. Kaper J. M. 1975 In The Chemical Basis of Virus Structure, Dissociation and Reassembly Amsterdam: North-Holland/American Elsevier;
    [Google Scholar]
  16. Kaper J. M., Alting Siberg R. 1969; The effect of freezing on the structure of turnip yellow mosaic virus and a number of simple plant viruses. Cryobiology 5:366–374
    [Google Scholar]
  17. Klug A., Longley W., Leberman R. 1966; Arrangement of protein subunits and the distribution of nucleic acid in turnip yellow mosaic virus. I. X-ray diffraction studies. Journal of Molecular Biology 15:315–343
    [Google Scholar]
  18. Lyttleton J. W., Matthews R. E. F. 1958; Release of nucleic acid from turnip yellow mosaic virus under mild conditions. Virology 6:460–471
    [Google Scholar]
  19. Matthews R. E. F., Ralph R. K. 1966; Turnip yellow mosaic virus. Advances in Virus Research 12:273–328
    [Google Scholar]
  20. Maurizot J. C., Blicharsky J., Brahms J. 1971; Comparison entre oligoribonucleotides et oligodesoxi-ribonucleotides. I. Formation des doubles helices. Biopolymers io:1429–1454
    [Google Scholar]
  21. Piazzolla P., Gallitelli D., Quacquarelli A. 1977a; The behaviour of some isometric plant viruses heated in vitro as determined by particle stabilizing forces. Journal of General Virology 37:373–384
    [Google Scholar]
  22. Piazzolla P., Guantieri V., Vovlas C., Tamburro A. M. 1977b; Circular dichroism studies of chicory yellow mottle virus. Journal of General Virology 37:359–372
    [Google Scholar]
  23. Schellman J. A., Lowe M. J. 1968; The optical rotation of ribonuclease. Journal of the American Chemical Society 90:1070–1072
    [Google Scholar]
  24. Simons E. R., Blout R. 1968; Circular dichroism of ribonuclease A, ribonuclease S and some fragments. Journal of Biological Chemistry 243:218–221
    [Google Scholar]
  25. Tamburro A. M., Scatturin A., Moroder L. 1968a; Far-ultraviolet optical rotatory dispersion and circular dichroism studies of bovine pancreatic ribonuclease A. Biochimica et Biophysica Acta 154:583–585
    [Google Scholar]
  26. Tamburro A. M., Scatturin A., Rocchi R. 1968b; A conformational study of pepsin and trypsin. Gaz-zetta Chimica Italiana 98:1256–1260
    [Google Scholar]
  27. Turano T. A., Hartman K. A., Thomas G. I. 1976; Studies of virus structure by Laser-Raman spectroscopy. 3. Turnip yellow mosaic virus. Journal of Physical Chemistry 80:1157–1163
    [Google Scholar]
  28. Yang J. T., Samejima T. 1969; Optical rotatory dispersion and circular dichroism of nucleic acids. Progress in Nucleic Acids Research 9:223–300
    [Google Scholar]
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