1887

Abstract

SUMMARY

An RNA polymerase activity has been demonstrated in purified rabies virions. Efficiency of the reaction is low since the rate of incorporation was equal to 3 to 5 pmol of uridine per hour, per mg of protein. As with other mammalian rhabdoviruses the optimal temperature was 31 °C. Unlike vesicular stomatitis virus, manganese could be substituted for magnesium as a divalent cation, at an optimum concentration of 10 to 20 m.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-40-1-233
1978-07-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/40/1/JV0400010233.html?itemId=/content/journal/jgv/10.1099/0022-1317-40-1-233&mimeType=html&fmt=ahah

References

  1. Aaslestad H. G., Clark H. F., Bishop D. H. L., Koprowski H. 1971; Comparison of the ribonucleic acid polymerases of two rhabdoviruses, Kern Canyon virus and vesicular stomatitis virus. Journal of Virology 7:726–735
    [Google Scholar]
  2. Adamowicz Ph., Legrand B., Guerche J., Prunet P. 1974; Un nouveau procѐdѐ de concentration et de purification de virus. Application au virus de la fiѐvre aphteuse produit sur cellule BHK 21, pour l’obtention de vaceins hautement purifies. Bulletin de l’Office International des Epizooties 81: (11–12) 1125–1150
    [Google Scholar]
  3. Baltimore D., Huang A. S., Stampfer M. 1970; Ribonucleic acid synthesis of vesicular stomatitis virus. II An RNA polymerase in the virion. Proceedings of the National Academy of Sciences of the United States of America 66:572–576
    [Google Scholar]
  4. Bishop D. H. L., Flamand A. 1975; Transcription process of animal RNA viruses. In Control process in virus multiplication pp 95–152 Edited by Burke D. C., Russell W. C. Cambridge: Cambridge University Press;
    [Google Scholar]
  5. Chang S. H., Hefti E., Obijeski J. F., Bishop D. H. L. 1974; RNA transcription by the virion polymerase of five rhabdoviruses. Journal of Virology 13:652–661
    [Google Scholar]
  6. Ermine A. 1978 In Negative Strand Viruses (in the press) London: Academic Press;
    [Google Scholar]
  7. Ermine A., Flamand A. 1977; RNA syntheses in BHK 21 cells infected by rabies virus. Annales de Microbiologie (Institut Pasteur) 128A:477–488
    [Google Scholar]
  8. Fiszman M. 1976; Anatomie chimique des virus. Les proteines. In Les Virus pp 69–82 Institut Pasteur (MacGraw Hill, Edition)
    [Google Scholar]
  9. Flamand A., Pese D., Bussereau F. 1977; Effect of actinomycin D and cytosine arabinoside on rabies and VSV multiplication. Virology 78:323–327
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. McAllister P. E., Wagner R. R. 1977; Virion rna polymerases of two salmonid rhabdoviruses. Journal of Virology 22:839–843
    [Google Scholar]
  12. Roy P., Bishop D. H. L. 1971; Nucleoside triphosphate phosphotransferase. A new enzyme activity of oncogenic and non oncogenic “budding” viruses. Biochimica et Biophysica Acta 235:191–206
    [Google Scholar]
  13. Roy P., Clark H. F., Madore H. P., Bishop D. H. L. 1975; RNA polymerase associated with virions of pike fry rhabdovirus. Journal of Virology 15:338–347
    [Google Scholar]
  14. Sokol F., Schlumberger H. D., Wiktor T. J., Koprowski H. 1969; Biochemical and biophysical studies on the nucleocapsid and on the RNA of rabies virus. Virology 38:651–665
    [Google Scholar]
  15. Sokol F., Stancek D., Koprowski H. 1971; Structural proteins of rabies virus. Journal of Virology 7:241–249
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-40-1-233
Loading
/content/journal/jgv/10.1099/0022-1317-40-1-233
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error