1887

Abstract

SUMMARY

Primary rat embryo cells were transformed by adenovirus 1. The transformed cells formed foci of multilayered growth in monolayer cultures; cell lines were derived from such foci. A virus stock containing 1.1 × 10 virus particles and 1.5 × 10 p.f.u./ml. contained 2.3 × 10 focus-forming units/ml. when assayed in rat embryo cells. Approximately 4.8 × 10 total virus particles or 6.5 × 10 p.f.u. were required to induce one focus of transformed cells. The transformation rate was 0.0003% for cells exposed to about 2 p.f.u./cell. The transformed cells contained adenovirus 1 specific tumour antigens when tested by immunofluorescent and complement-fixation reactions. They also synthesized adenovirus 1 specific RNA which forms RNase-resistant hybrids with adenovirus 1 DNA.

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/content/journal/jgv/10.1099/0022-1317-4-1-29
1969-01-01
2022-06-29
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References

  1. Berman L. D. 1967; Comparative morphologic study of the virus-induced solid tumors of Syrian hamsters. J. natn. Cancer Inst 39:847
    [Google Scholar]
  2. Black P. H., Lewis A. M. Jun., Blacklow N. R., Austin J. B., Rowe W. P. 1967; The presence of adenovirus-specific antigens in hamster cells rendered neoplastic by adenovirus i-SV 40 and adenovirus 2-SV 40 hybrid viruses. Proc. natn. Acad. Sci. U. S. A 57:1324
    [Google Scholar]
  3. Freeman A. E., Black P. H., Walford R., Huebner R. J. 1967b; The adenovirus type 12-rat embryo transformation system. J. Virol 1:362
    [Google Scholar]
  4. Freeman A. E., Vanderpool E. A., Black P. H., Turner H. C., Huebner R. J. 1967c; Transformation of primary rat embryo cells by weakly oncogenic adenovirus type 3. Nature, Lond 216:171
    [Google Scholar]
  5. Freeman A. E., Black P. H., Vanderpool E. A., Henry P. H., Austin J. B., Huebner R. J. 1967a; Transformation of primary rat embryo cells by adenovirus type 2. Proc. natn. Acad. Sci. U.S.A 58:1205
    [Google Scholar]
  6. Fujinaga K., Green M. 1966; The mechanism of viral carcinogenesis by DNA mammalian viruses: viral-specific RNA in polyribosomes of adenovirus tumor and transformed cells.. Proc. natn. Acad. Sci. U.S.A. 55:1567
    [Google Scholar]
  7. Gilden R. V., Beddow T. G., Huebner R. J. 1967; Production of high-titer antibody in serum and ascitic fluid of hamsters for a variety of virus-induced tumour antigens. Appl. Microbiol 15:657
    [Google Scholar]
  8. Gillespie D., Spiegelman S. 1965; A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol 12:829
    [Google Scholar]
  9. Huebner R. J., Rowe W. P., Turner H. C., Lane W. T. 1963; Specific adenovirus complement-fixing antigens in virus-free hamster and rat tumours. Proc. natn. Acad. Sci. U.S.A 50:379
    [Google Scholar]
  10. Lewis A. M. Jun, Wiese W. H., Rowe W. P. 1967; The presence of antibodies in human serum to early (T) adenovirus antigens. Proc. natn. Acad. Sci. U.S.A. 57:622
    [Google Scholar]
  11. Lewis A. M. Jun., Baum S. G., Prigge K. O., Rowe W. P. 1966; Occurrence of adenovirus-SV 40 hybrids among monkey kidney cell adapted strains of adenovirus. Proc. Soc. exp. BiolMed 122:214
    [Google Scholar]
  12. McAllister R. M., MacPherson I. 1968; Transformation of a hamster cell line by adenovirus type 12. J. gen. Virol 2:99
    [Google Scholar]
  13. McAllister R. M., Landing B. H., Goodheart C. R. 1964; Isolation of adenoviruses from neoplastic and non-neoplastic tissues of children. Lab. Invest 13:894
    [Google Scholar]
  14. McAllister R. M., Reed G., Huebner R. J. 1967; Colonial growth in agar of cell derived from adenovirus-induced hamster tumours. J. natn. Cancer Inst 39:43
    [Google Scholar]
  15. Macpherson I., Montagnier L. 1964; Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23:291
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. molec. Biol 3:208
    [Google Scholar]
  17. Pope J. H., Rowe W. P. 1964; Immunofluorescent studies of adenovirus 12 tumours and of cells transformed or infected by adenoviruses. J. exp. Med 120:577
    [Google Scholar]
  18. Scherrer K., Latham H., Darnell J. E. 1963; Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells. Proc. natn. Acad. Sci. U.S.A 49:240
    [Google Scholar]
  19. Watson D. H., Russell W. C., Wildy P. 1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250
    [Google Scholar]
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