1887

Abstract

SUMMARY

Changes induced in the ionic composition of the surface of herpes simplex virus (HSV) infected cells were evaluated by whole cell electrophoresis and isoelectric focusing. The kinetics of the progressive decline in cell electrophoretic mobility (a measurement of negative surface charge density) after infection corresponded to the production of infectious virus. The observed HSV-induced increase in cell isoelectric focusing pH demonstrated that the electrokinetic cell surface became ionically more positive. The nature of the ionic changes were investigated using a series of specific reagents in conjunction with cell electrophoresis. Infected cells had 2.4 times more surface binding sites for the amino-specific reagent 4-acetamide-4′-isothiocyanostilbene-2,2′-disulphonic acid. A relationship between these virus-induced amino groups and HSV surface antigens was indicated by the similar kinetics of their appearance and electrophoretic neutralization by HSV antibodies.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-39-3-449
1978-06-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/39/3/JV0390030449.html?itemId=/content/journal/jgv/10.1099/0022-1317-39-3-449&mimeType=html&fmt=ahah

References

  1. Ambrose E. J. 1966; Electrophoretic behavior of ceils. Progress in Biophysics and Molecular Biology 16:243–265
    [Google Scholar]
  2. Boltz R. C., Todd P., Streibel M. J., Louie M. K. 1973; Preparative electrophoresis of living mammalian cells in a stationary Ficoll gradient. Preparative Biochemistry 3:383–401
    [Google Scholar]
  3. Boltz R. C. Jun, Todd P., Hammerstedt R., Hymer W. C., Thompson C. J., Docherty J. J. 1977; Initial studies on the separation of cells by density gradient isoelectric focusing. Proceedings of the International Symposium on Cell Separation Methods Edited by Bloemendal H. Amsterdam: Elsevier;
    [Google Scholar]
  4. Brier A. M., Wohlenberg C., Rosenthal J., Mage M., Notkins A. L. 1971; Inhibition or enhancement of immunological injury of virus infected cells. Proceedings of the National Academy of Sciences of the United States of America 12:3073–3077
    [Google Scholar]
  5. Cook G. M. W., Stoddart R. W. 1973 In Surface Carbohydrates of the Eukaryotic Cell pp 58–64 New York: Academic Press;
    [Google Scholar]
  6. Curtis A. S. 1967; In. The Cell Surface: its Molecular Role in Morphogenesis chapter 2 pp 51–79 New York & London: Academic Press;
    [Google Scholar]
  7. Deman J. J., Bruyneel E. A. 1974; Evidence for long range electrostatic repulsion between HeLa cells. Experimental Cell Research 89:206–216
    [Google Scholar]
  8. Docherty J. J., Goldberg R. J., Rapp F. 1971; Differential effect of 7, 12-dimethylbenz(a)anthracene on infectivity of herpes simplex virus type 2. Proceedings of the Society for Experimental Biology and Medicine 136:328–333
    [Google Scholar]
  9. Ejercito P. M., Kieff E. D., Roizman B. 1968; Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. Journal of General Virology 2:357–364
    [Google Scholar]
  10. Forrester J. A., Dumonde D. C., Ambrose E. J. 1965; The effect of antibodies on cells. II. Changes in the electrophoretic mobility of ascites tumor cells treated with antibodies and complement. Immunology 8:37–48
    [Google Scholar]
  11. Fritz M. E., Nahmias A. J. 1972; Reversed polarity in transmembrane potentials of cells infected with herpes viruses. Proceedings of the Society for Experimental Biology and Medicine 139:1159–1161
    [Google Scholar]
  12. Gray A., Tokumaru T., Scott T. F. 1958; Different cytopathogenic effects observed in HeLa cells infected with herpes simplex virus. Archiv für die gesamte Virusforschung 8:59–65
    [Google Scholar]
  13. Hartman R. S., Nungester W. J. 1953; An electrophoretic method for titration of antisera and its application to anti-tumour sera. Proceedings of the Society for Experimental Biology and Medicine 84:710–713
    [Google Scholar]
  14. Heine J. W., Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane. Journal of Virology 9:431–439
    [Google Scholar]
  15. Kamiya T., Ben-Porat T., Kaplan A. S. 1965; Control of certain aspects of the infective cell process by viral DNA. Virology 26:577–589
    [Google Scholar]
  16. Kaplan A. S., Erickson I. S., Ben-Porat T. 1975; Synthesis of proteins in cells infected with herpes virus. Proteins excreted by cells infected with herpes simplex virus, types 1 and 2. Virology 64:132–143
    [Google Scholar]
  17. Kornguth S. E., Stahmann M. A., Anderson J. W. 1961; Effect of polylysine on the cytology of Ehrlich ascites tumor cells. Experimental Cell Research 24:484–494
    [Google Scholar]
  18. Maddy A. H. 1969; A fluorescent label for the outer components of the plasma membrane. Biochimica et Biophysica Acta 88:390–399
    [Google Scholar]
  19. Mayhew E., Harlos J. P., Juliano L. R. 1973; The effect of polycations on cell membrane stability and transport processes. Journal of Membrane Biology 14:213–228
    [Google Scholar]
  20. Mehrishi J. N. 1972; Molecular aspects of the mammalian cell surface. Progress in Biophysics and Molecular Biology 25:1–70
    [Google Scholar]
  21. Moore H. D., Hibbitt K. G. 1975; Isoelectric focusing of boar spermatozoa. Journal of Reproduction and Fertility 44:329–332
    [Google Scholar]
  22. Munk K., Saur G. 1964; Relationship between cell DNA metabolism and nucleocytoplasmic alterations in herpes virus-infected cells. Virology 22:153–154
    [Google Scholar]
  23. O’dea J. F., Dineen J. F. 1957; Fluorescent antibody studies with herpes simplex virus in preparations of unfixed tissue cultures. Journal of General Microbiology 17:19–24
    [Google Scholar]
  24. Olofsson S. 1975; Isoelectric focusing of herpes simplex virus. Archiv für die gesamte Virusforschung 49:93–98
    [Google Scholar]
  25. Roane P. R. Jun, Roizman B. 1964; Studies of the determinant antigens of viable cells. II. Demonstration of altered antigenic reactivity of HEp-2 cells infected with herpes simplex virus. Virology 22:1–8
    [Google Scholar]
  26. Robinson W. S., Pitkanen A., Rubin H. 1965; The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United States of America 54:137–144
    [Google Scholar]
  27. Roizman B., Furlong D. 1974; The replication of herpes viruses. In Comprehensive Virology vol 3 pp 229–403 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  28. Roizman B., Heine J. W. 1972; Modification of human cell membranes by herpes viruses. In Membrane Research pp 203–223 Edited by Fox C. F. New York & London: Academic Press;
    [Google Scholar]
  29. Ryser H. J. 1968; Uptake of protein by mammalian cells: an underdeveloped area. Science 159:390–396
    [Google Scholar]
  30. Savage T., Roizman B., Heine J. W. 1972; Immunological specificity of the glycoproteins of subtypes 1 and 2. Journal of General Virology 17:31–48
    [Google Scholar]
  31. Shore S. L., Nahmias A. J., Starr S. E., Wood P. A., Mcfarlin D. E. 1974; Detection of cell dependent cytotoxic antibody to cells infected with herpes simplex virus. Nature, London 251:350–352
    [Google Scholar]
  32. Tenforde T., Clarke E. J., Macgregor R. D. Jun, Streibel M. J., Todd P. W. 1973; A Convenient microelectrophoresis assembly. Medical and Biological Engineering n:236–238
    [Google Scholar]
  33. Wagner E. K., Roizman B. 1969; RNA synthesis in cells infected with herpes simplex virus. I. The patterns of RNA synthesis in productivity infected cells. Journal of Virology 4:36–46
    [Google Scholar]
  34. Watkins J. F. 1965; The relationship of herpes simplex haemadsorption phenomenon to the virus growth cycle. Virology 26:746–753
    [Google Scholar]
  35. Wilbanks G. D., Campbell J. A. 1972; Effect of herpes virus homines type 2 on the human cervical epithelium: scanning electron microscopes observations. American Journal of Obstetrics and Gynecology 112:924–929
    [Google Scholar]
  36. Weiss L. 1969; The cell periphery. International Review of Cytology 20:63–105
    [Google Scholar]
  37. Westmoreland D., Watkins J. F. 1974; The IgG receptor induced by herpes simplex virus: studies using radioiodinated IgG. Journal of General Virology 24:167–178
    [Google Scholar]
  38. Yasuda J., Milgrom F. 1968; Haemadsorption by herpes simplex infected cell cultures. International Archives of Allergy and Applied Immunology 33:151–170
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-39-3-449
Loading
/content/journal/jgv/10.1099/0022-1317-39-3-449
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error