Bacteriophage 6 nucleic acid was present as a torus after chromic acid-formaldehyde-OsO fixation and acetone and propylene oxide dehydration. A herpes virus, infectious bovine rhinotracheitis virus, had its DNA mostly as a torus, collapsed in the centre, or as a network, after glutaraldehyde-OsO fixation, but in an uncollapsed torus or network formation after chromic acid-formaldehyde-OsO. This fixative stabilized nucleic acids, allowing acetone dehydration and plastic embedding without collapse of nucleic acid to the centre of the virion.


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