1887

Abstract

SUMMARY

Bacteriophage 6 nucleic acid was present as a torus after chromic acid-formaldehyde-OsO fixation and acetone and propylene oxide dehydration. A herpes virus, infectious bovine rhinotracheitis virus, had its DNA mostly as a torus, collapsed in the centre, or as a network, after glutaraldehyde-OsO fixation, but in an uncollapsed torus or network formation after chromic acid-formaldehyde-OsO. This fixative stabilized nucleic acids, allowing acetone dehydration and plastic embedding without collapse of nucleic acid to the centre of the virion.

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/content/journal/jgv/10.1099/0022-1317-39-2-377
1978-05-01
2022-01-18
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References

  1. Furlong D., Swift H., Roizman B. 1972; Arrangement of herpesvirus deoxyribonucleic acid in the core. Journal of Virology 10:1071–1074
    [Google Scholar]
  2. Gonzalez C. F., Langenberg W. G., Van Etten J. L., Vidaver A. K. 1977; Ultrastructure of bacteriophage ϕ6: arrangement of the double-stranded RNA and envelope. Journal of General Virology 35:353–359
    [Google Scholar]
  3. Hopwood D. 1973; Recent advances in fixation of tissues. In Electron Microscopy and Cytochemistry pp 367–381 Edited by Wisse E., Daems W. Th., Molenaar I., Van Duijn P. Amsterdam: North-Holland Publishing Co;
    [Google Scholar]
  4. Johansen D. A. 1940 In Plant Microtechnique pp 523 New York: McGraw-Hill Book Co., Inc;
    [Google Scholar]
  5. Langenberg W. G., Sharper R. L. 1977; Stabilization of bacteriophage ϕ6 and infectious bovine rhinotracheitis virus nucleic acids by fixation with buffered chromic acid-formaldehyde. Annual Proceedings of the American Phytopathological Society pp 150–151
    [Google Scholar]
  6. Langenberg H. F., Schroeder W. G., Welch A. B., Cook G. E. 1972; Epoxy film separable from glass surfaces for selective light and electron microscopy of tissue and in situ grown cells. Stain Technology 47:303–308
    [Google Scholar]
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