1887

Abstract

SUMMARY

Although human embryo kidney (HEK), muscle (HEM) and lung (HEL) cells are capable of supporting the replication of BK virus (BKV) through passage levels 9, 12 and 12 respectively, only third, fourth and fifth passage level HEK cells were found to be satisfactory for the plaque assay of the virus. BSC-1 and VERO cells can also be used for the plaque assay of BKV. However, in HEK cells plaques can be visualized in 20 days (compared to 28 days in BSC-1 cells), and since in VERO cells the plaques are poorly defined and the titre about 1 log lower than in either HEK or BSC-1 cells, HEK cells were the ones chosen for use.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-38-2-383
1978-02-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/38/2/JV0380020383.html?itemId=/content/journal/jgv/10.1099/0022-1317-38-2-383&mimeType=html&fmt=ahah

References

  1. Dougherty R. M., Di Stefano H. S. 1974; Isolation and characterization of a papovavirus from human urine. Proceedings of the Society for Experimental Biology and Medicine 146:481–487
    [Google Scholar]
  2. Dulbecco R., Freeman G. 1959; Plaque production by the polyoma virus. Virology 8:396–397
    [Google Scholar]
  3. Gardner S. D., Field A. M., Coleman D. V., Hulme B. 1971; New human papovavirus (BK) isolated from urine after renal transplantation. Lancet i:1253–1257
    [Google Scholar]
  4. Major E. O., Di Mayorca G. 1973; Malignant transformation of BHK21 clone 13 cells by BK virus - a human papovavirus. Proceedings of the National Academy of Sciences of the United States of America 70:3210–3212
    [Google Scholar]
  5. Riggs J. L., Lennette E. H. 1965; Simian virus 40: isolation of two plaque types. Science 147:408–409
    [Google Scholar]
  6. Seehafer J., Salmi A., Scraba D. G., Colter J. S. 1975; A comparative study of BK and polyoma viruses. Virology 66:192–205
    [Google Scholar]
  7. Sheinin R. 1961; A rapid plaque assay for polyoma virus. Virology 15:85–87
    [Google Scholar]
  8. Stinebaugh S., Melnick J. L. 1962; Plaque formation by vacuolating virus, SV40. Virology 16:348–350
    [Google Scholar]
  9. Takemoto K. K., Todaro G. J., Habel K. 1968; Recovery of SV40 virus with genetic markers of original inducing virus from SV40-transformed mouse cells. Virology 35:1–8
    [Google Scholar]
  10. Van Der Noordaa J. 1976; Infectivity, oncogenicity and transforming ability of BK virus and BK virus DNA. Journal of General Virology 30:371–373
    [Google Scholar]
  11. Wildy P., Stoker M. G. P., Macpherson I. A., Horne R. W. 1960; The fine structure of polyoma virus. Virology u:444–457
    [Google Scholar]
  12. Winocour E., Sachs L. 1959; A plaque assay for the polyoma virus. Virology 8:397–400
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-38-2-383
Loading
/content/journal/jgv/10.1099/0022-1317-38-2-383
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error