1887

Abstract

SUMMARY

Several factors were investigated in relation to infection and multiplication of clover yellow mosaic virus (CYMV) in cowpea mesophyll protoplasts. The extent of virus multiplication was determined by fluorescent antibody staining and infectivity assay. The conditions that gave the most infection (38%) were: preincubation of the virus with 0.4 µg/ml poly--ornithine, a virus concentration of 4 µg/ml, an inoculum containing citrate buffer at pH 6.0, and contact between inoculum and protoplasts for at least 15 min. Time-course studies revealed synchronous one-step growth of virus with rapid accumulation of virus antigen from 12 to 48 h after inoculation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-38-2-303
1978-02-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/38/2/JV0380020303.html?itemId=/content/journal/jgv/10.1099/0022-1317-38-2-303&mimeType=html&fmt=ahah

References

  1. Aoki S., Takebe I. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39:439–448
    [Google Scholar]
  2. Beier H., Bruening G. 1975; The use of an abrasive in the isolation of cowpea leaf protoplasts which support the multiplication of cowpea mosaic virus. Virology 64:272–276
    [Google Scholar]
  3. Campbell D. H., Garvey J. H., Cremer N. E., Sussdorf D. H. 1970; Isolation of rabbit antibodies and their subunits. In Methods in Immunology pp 183–234 New York: W. A. Benjamin;
    [Google Scholar]
  4. Fenner F. 1976; The classification and nomenclature of viruses. Summary of results of meetings of the International Committee on Taxonomy of Viruses in Madrid, September, 1975. Virology 71:371–378
    [Google Scholar]
  5. Hibi T., Yora K. 1972; Electron microscopy of tobacco mosaic virus infection in tobacco mesophyll protoplasts. Annals of the Phytopathological Society of Japan 38:350–356
    [Google Scholar]
  6. Hibi T., Rezelman G., Van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
    [Google Scholar]
  7. Hiruki C., Shukla P., Rao D. V. 1976; Occurrence of clover yellow mosaic virus aggregates in transfer cells of Pisum sativum. Phytopathology 66:594–597
    [Google Scholar]
  8. Huber R., Rezelman G., Hibi T., Van Kammen A. 1977; Cowpea mosaic virus infection of protoplasts from Samsun tobacco leaves. Journal of General Virology 34:315–323
    [Google Scholar]
  9. Koike S., Hibi T., Yora K. 1976; Infection of cowpea mesophyll protoplasts by TMV. Annals of the Phytopathological Society of Japan 42:105
    [Google Scholar]
  10. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
    [Google Scholar]
  11. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  12. Okuno T., Furusawa I., Hiruki C. 1977; Infection of barley protoplasts with brome mosaic virus. Phytopathology 67:610–615
    [Google Scholar]
  13. Otsuki Y., Takebe I. 1969; Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts. Virology 38:497–499
    [Google Scholar]
  14. Otsuki Y., Takebe I. 1973; Infection of tobacco mesophyll protoplasts by cucumber mosaic virus. Virology 52:433–438
    [Google Scholar]
  15. Otsuki Y., Takebe I., Honda Y., Kajita S., Matsui C. 1974; Infection of tobacco mesophyll protoplasts by potato virus X. Journal of General Virology 22:375–385
    [Google Scholar]
  16. Purcifull D. E., Shepherd R. J. 1964; Preparation of protein fragments of several rod-shaped plant viruses and their use in agar-gel diffusion tests. Phytopathology 54:1102–1108
    [Google Scholar]
  17. Renaudin J., Bove J. M., Otsuki Y., Takebe I. 1975; Infection of brassica leaf protoplasts by turnip yellow mosaic virus. Molecular and General Genetics 141:59–68
    [Google Scholar]
  18. Takebe I. 1975; The use of protoplasts in plant virology. Annual Review of Phytopathology 13:105–125
    [Google Scholar]
  19. Takebe I., Otsuki Y. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Sciences of the United States of America 64:843–848
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-38-2-303
Loading
/content/journal/jgv/10.1099/0022-1317-38-2-303
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error