1887

Abstract

SUMMARY

A long-tailed and generalized transducing phage ES18 can recombine with a serologically unrelated phage . When ES18 phage stocks grown on lysogens were plated on lysogens resistant to ES18, plaques of a new hybrid phage P181 were found at a frequency of about 10. Serological analysis of phage P181 showed that it carries the protein coat of and has no serological cross-reactivity with ES18. Phage P181 expresses the clear plaque morphology similar to that of ES18. ES18 was also found to carry a homology with P22 in at least the regions. Thus, the markers (+, 1, 2 and 3) of P22 can be transferred to ES18. When these ES18 strains were used for isolation of P181 phage, the clear plaque morphology of P181 mimicked that of the P22 marker in the ES18 strain which gave rise to the P181 strain. strain Q1, lysogenic for P181, is immune to ES18 but not to . Furthermore, ES18 lysogens are immune to P181. Therefore it is concluded that the hybrid phage P181 conserves the protein coat of and carries at least the regions of ES18.

When ES18 was grown on lysogens of recombination-deficient () mutants, P181 hybrid particles were found in the ES18 stocks at a frequency of about 10. This frequency is about 1000-fold lower than that found in ES18 stocks grown on the wild type hosts lysogenic for . Therefore, it is concluded that the bacterial recombination mechanism plays an important role in the formation of P181 by recombination between the prophage and the superinfecting phage ES18.

Although phage ES18 belongs to B group phages, it possesses a generalized transducing capability. We have studied a possible origin of ES18.

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1978-02-01
2024-12-09
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References

  1. Boyd J. S. K. 1950; The symbiotic bacteriophages of Salmonella typhimurium. Journal of Pathology and Bacteriology 72:501–517
    [Google Scholar]
  2. Boyd J. S. K., Bid Well D. E. 1957; The type A phages of Salmonella typhimurium: identification by a standardized cross-immunity test. Journal of General Microbiology 16:217–228
    [Google Scholar]
  3. Callow B. R. 1959; A new phage-typing scheme for Salmonella typhimurium. Journal of Hygiene 57:346–359
    [Google Scholar]
  4. Fukuda S., Yamamoto N. 1972a; A restriction sensitive mutant of Salmonella phage P22. Bacteriological Proceedings p 237
    [Google Scholar]
  5. Fukuda S., Yamamoto N. 1972b; Formation of various genome lengths of hybrids between the serologically and morphologically unrelated bacteriophage species. Virology 50:727–732
    [Google Scholar]
  6. Gemski P., Baron L. S., Yamamoto N. 1972; Formation of hybrids between coliphage y and Salmonella phage P22 with a Salmonella typhimurium hybrid sensitive to these phages. Proceedings of the National Academy of Sciences of the United States of America 69:3110–3114
    [Google Scholar]
  7. Kuo T., Stocker B. A. D. 1970; ES18, a general transducing phage for smooth and nonsmooth Salmonella typhimurium. Virology 42:621–632
    [Google Scholar]
  8. Smith H. O., Levine M. 1967; A phage P22 gene controlling integration of prophage. Virology 31:207–216
    [Google Scholar]
  9. Spicer C. C., Datta N. 1959; Reversion of transduced antigenic characters in Salmonella typhimurium. Journal of General Microbiology 20:136–143
    [Google Scholar]
  10. Thomas C. A. 1967; The rule of the ring. Journal of Cellular Physiology 70: suppl 113–14
    [Google Scholar]
  11. Yamagami H., Yamamoto N. 1970; Contribution of the bacterial recombination function to replication of bacteriophage P22. Journal of Molecular Biology 53:281–285
    [Google Scholar]
  12. Yamamoto N. 1967; The origin of bacteriophage P221. Virology 33:545–547
    [Google Scholar]
  13. Yamamoto N. 1969a; Genetic evolution of bacteriophage. I. Hybrids between unrelated bacteriophages P22 and Fels 2. Proceedings of the National Academy of Sciences of the United States of America 62:63–69
    [Google Scholar]
  14. Yamamoto N. 1969b; Damage, repair and recombination. I. Contribution of the host to recombination between the superinfecting phage and the prophage. Virology 38:447–456
    [Google Scholar]
  15. Yamamoto N. 1970; Generalized transducing phage ES18 recombines with other phage species. Bacteriological Proceedings p 202
    [Google Scholar]
  16. Yamamoto N., Anderson T. F. 1961; Genomic masking and recombination between serologically unrelated phages P22 and P221. Virology 14:430–439
    [Google Scholar]
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