1887

Abstract

SUMMARY

Various double-stranded RNAs of either synthetic or natural origin have been compared for their interferon-inducing potency in human skin fibroblasts ‘primed’ with interferon and ‘superinduced’ with cycloheximide and actinomycin D. While natural double-stranded RNAs (extracted from either mycophage, f bacteriophage or reovirus) and alternating copolymers [(A-U).(A-U), (G-C).(G-C), (I-C).(I-C)]* proved relatively less effective in inducing interferon than (I).(C), a variety of synthetic homopolymer pairs, including (I).(brC), (I).(sC), (A).(rT) and (A).(U), showed an interferon-inducing activity comparable to that of (I).(C).

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1977-12-01
2022-01-17
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References

  1. Billiau A., Joniau M., De Somer P. 1973; Mass production of human interferon in diploid cells stimulated by poly-I: C. Journal of General Virology 19:1–8
    [Google Scholar]
  2. Black D. R., Eckstein F., De Clercq E., Merigan T. C. 1973; Studies on the toxicity and antiviral activity of various polynucleotides. Antimicrobial Agents and Chemotherapy 3:198–206
    [Google Scholar]
  3. Buck K. W., Chain E. B., Himmelweit F. 1971; Comparison of interferon induction in mice by purified Penicillium chrysogenum virus and derived double-stranded RNA. Journal of General Virology 12:131–139
    [Google Scholar]
  4. Burnett J. P., Frank B. H., Douthart R. J. 1975; Ionic strength effects on the stability and conformation of Penicillium chrysogenum mycophage double stranded RNA. Nucleic Acids Research 2:759–771
    [Google Scholar]
  5. Colby C., Chamberlin M. J. 1969; The specificity of interferon induction in chick embryo cells by helical RNA. Proceedings of the National Academy of Sciences of the United States of America 63:160–167
    [Google Scholar]
  6. De Clercq E. 1974; Synthetic interferon inducers. Topics in Current Chemistry 52:173–208
    [Google Scholar]
  7. De Clercq R. D., Wells E., Merigan T. C. 1970; Increase in antiviral activity of polynucleotides by thermal activation. Nature, London 226:364–366
    [Google Scholar]
  8. De Clercq E., Torrence P. F., Witkop B. 1974; Interferon ainduction by synthetic polynucleotides: importance of purine N-7 and strandwise rearrangement. Proceedings of the National Academy of Sciences of the United States of America 71:182–86
    [Google Scholar]
  9. De Clercq E., Edy V. G., Torrence P. F., Waters J. A., Witkop B. 1976; Antiviral activity of poly(7-deazainosinic acid)-derived complexes in vitro and in vivo . Molecular Pharmacology 12:1045–1051
    [Google Scholar]
  10. Doskočil J., Fuchsberger N., Vetrak J., Lackovič V., Borecky L. 1971; Double-stranded f2 phage RNA as interferon inducer. Acta Virologica 15:523
    [Google Scholar]
  11. Field A. K., Lampson G. P., Tytell A. A., Nemes M. N., Hilleman M. R. 1967; Inducers of interferon and host resistance, IV. double-stranded replicative form RNA (MS2-RF-RNA) from E. coli infected with MS2 coliphage. Proceedings of the National Academy of Sciences of the United States of America 58:2102–2108
    [Google Scholar]
  12. Havell E. A., Vilček J. 1972; Production of high-titered interferon in cultures of human diploid cells. Antimicrobial Agents and Chemotherapy 2:476–484
    [Google Scholar]
  13. Lemke P. A., Ness T. M. 1970; Isolation and characterization of a double-stranded ribonucleic acid from Penicillium chrysogenum . Journal of Virology 6:813–819
    [Google Scholar]
  14. Long W. F., Burke D. C. 1971; Interferon production by double-stranded RNA: a comparison of induction by reovirus to that by a synthetic double-stranded polynucleotide. Journal of General Virology 12:1–11
    [Google Scholar]
  15. Nash C. H., Douthart R. J., Ellis L. F., Van frank R. M., Burnett J. P., Lemke P. A. 1973; On the mycophage of Penicillium chrysogenum . Canadian Journal of Microbiology 19:97–103
    [Google Scholar]
  16. Novokhatsky A. S., Ershov F. I., Timkovsky A. L., Bresler S. E., Kogan E. M., Tikhomirova-Sidorovan S. 1975; Double-stranded complex of polygyanylic and polycytidylic acids and its antiviral activity in tissue culture. Acta Virologica 19:121–129
    [Google Scholar]
  17. Reuss K., Scheit K.-H., Saiko O. 1976; Induction of interferon by polyribonucleotides containing thio-pyrimidines. Nucleic Acids Research 3:2861–2875
    [Google Scholar]
  18. Stewart H. W. E., De Clercq E. 1974; Relationship of cytotoxicity and interferon-inducing activity of polyriboinosinic acid. polyribocytidylic acid to the molecular weights of the homopolymers. Journal of General Virology 23:83–89
    [Google Scholar]
  19. Taborsky I., Doskočil J., Zajicova V. 1974; Pyrogenic activity of double-stranded RNA from Escherichia coli f2 phage. Acta Virologica 18:106–112
    [Google Scholar]
  20. Thang M. N., Bachner L., De Clercq E., Stollar B. D. 1977; A continuous high-molecular weight base-paired structure is not an absolute requirement for a potential polynucleotide inducer of interferon. FEBS Letters 76:159–165
    [Google Scholar]
  21. Torrence P. F., De Clercq E. 1977; Inducers and induction of interferons. Pharmacology and Therapeutics (in the press)
    [Google Scholar]
  22. Torrence P. F., De Clercq E., Waters J. A., Witkop B. 1974; A potent interferon inducer derived from poly(7-deazainosinic acid). Biochemistry 13:4400–4408
    [Google Scholar]
  23. Tytell A. A., Lampson G. P., Field A. K., Hilleman M. R. 1967; Inducers of interferon and host resistance, III. double-stranded RNA from reovirus type 3 virions (reo 3-RNA). Proceedings of the National Academy of Sciences of the United States of America 58:1719–1722
    [Google Scholar]
  24. Tytell A. A., Lampson G. P., Field A. K., Nemes M. N., Hilleman M. R. 1970; Influence of size of individual homopolynucleotides on the physical and biological properties of complexed rln: rCn (poly I:C). Proceedings of the Society for Experimental Biology and Medicine 135917–921
    [Google Scholar]
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