1887

Abstract

Summary

Hydroxyurea (HU) treatment of HSV-infected cells markedly inhibits the synthesis of virus DNA. Only 0.36% of the H-thymidine label was incorporated into virus DNA in the presence of HU as compared to untreated infected cells. Removal of HU resulted in a renewed synthesis of virus DNA as determined by the gradual increase in the incorporation of H-thymidine into HSV DNA. The labelled virus DNA molecules were isolated and chromatographed on benzoylated napthoylated DEAE (BND)-cellulose columns to separate the replicative intermediates that have single-stranded (ss) sequences from the mature double-stranded (ds) DNA genomes. Mature radioactive dsDNA molecules were found to appear at 22 min after removal of HU and gradually increased in amount thereafter. The virus DNA molecules synthesized during the initial 20 min after removal of HU, constitute the replicative intermediates of HSV DNA. It was calculated that the synthesis of HSV DNA proceeds at the rate of about 5 × 10 daltons per min.

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/content/journal/jgv/10.1099/0022-1317-37-2-429
1977-11-01
2022-01-27
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References

  1. Becker Y. 1976; Antiviral drugs; mode of action and chemotherapy of viral infections of man. In Monographs in Virology no. 11 Basel: S. Karger;
    [Google Scholar]
  2. Bellet A. J. D., Younghusband H. B. 1972; Replication of the DNA of chick embryo lethal orphan virus. Journal of Molecular Biology 72:691–709
    [Google Scholar]
  3. Cordeiro M., Meneghini R. 1973; The rate of DNA replication in the polytene chromosomes of Rhyn-chosciara angelae. Journal of Molecular Biology 78:261–274
    [Google Scholar]
  4. Friedmann A., Shlomai J., Becker Y. 1977; Electron microscopy of HSV DNA molecules isolated from infected cells by centrifugation in CsCl density gradients. Journal of General Virology 34:507–522
    [Google Scholar]
  5. Kornberg A. 1974 In DNA synthesis p 294 San Francisco: W. H. Freeman;
    [Google Scholar]
  6. Krakoff L. H., Brown N. C., Reichard P. 1968; Inhibition of ribonucleoside diphosphate reductase by hydroxyurea. Cancer Research 38:1559–1565
    [Google Scholar]
  7. Lewis W. H., Wright J. A. 1974; Altered ribonucleotide reductase activity in mammalian tissue culture cells resistant to hydroxyurea. Biochemical and Biophysical Research Communications 60:926–933
    [Google Scholar]
  8. Nathans D., Danna K. L. 1972; Specific origin in SV40 DNA replication. Nature New Biology 336:200–202
    [Google Scholar]
  9. Olshevsky U. 1974; The proteins of herpes simplex virus. Ph.D. thesis The Hebrew University of Jerusalem;
    [Google Scholar]
  10. Pearson O. D. 1975; Intermediate in adenovirus type 2 replication. Journal of Virology 16:17–26
    [Google Scholar]
  11. Powell K. L., Purifoy D. J. M., Courtney R. J. 1975; The synthesis of herpes simplex virus proteins in in the absence of virus DNA synthesis. Biochemical and Biophysical Research Communications 66:262–271
    [Google Scholar]
  12. Rosenkranz H. S., Becker Y. 1973; Reversible inhibiton of herpes simplex virus replication by hydroxyurea. Antimicrobial Agents and Chemotherapy 3:325–331
    [Google Scholar]
  13. Shlomai J., Friedmann A., Becker Y. 1972a; Replicative intermediates of herpes simplex virus DNA. Virology 69:647–659
    [Google Scholar]
  14. Shlomai J., Strauss B., Asher Y., Friedmann A., Becker Y. 1976b; Analysis of herpes simplex virus DNA synthesized in infected nuclei by chromatography on benzoylated napthoylated DEAE cellulose columns. Journal of General Virology 32:189–204
    [Google Scholar]
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