1887

Abstract

Summary

The proteins of five herpesviruses — herpes simplex types 1 and 2 (HSV-1 and HSV-2), bovine mammillitis virus (BMV), pseudorabies virus (PRV) and equine abortion virus (EAV) — have been compared by polyacrylamide gel electrophoresis of purified virus and by antigenic analysis using virus neutralization and agar-gel immunodiffusion tests. Although there were general similarities in the number, size range and overall complexity of polypeptides separated by polyacrylamide gel electrophoresis from purified particles of the five viruses, no single component of identical mobility was present in all viruses. The major capsid polypeptide from each of the five viruses comprised about 10% of virion protein mass but had distinctive mol. wt. of 158000 to 160000 (HSV-2 and BMV), 145000 (EAV) and 140000 (PRV) relative to 155000 for HSV-1.

Despite differences of apparent mol. wt. there was a one-for-one correspondence for the majority of structural polypeptides of HSV-1 and HSV-2 and many polypeptides of BMV were recognizably analogous to those of HSV-1 and HSV-2. In contrast the polypeptides of PRV and, more particularly, EAV differed markedly from those of all the other viruses. The antigenic analysis complemented these results; HSV-1, HSV-2 and BMV all cross-neutralized, whereas PRV and EAV were only neutralized by homologous antisera. Agar gel immunodiffusion tests with extracts of infected cells demonstrated at least one antigen common to all five viruses and showed BMV to be more closely related to HSV-1 and HSV-2 than were PRV or EAV although less closely related than HSV-1 to HSV-2. PRV and EAV share more antigens with each other than with the other viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-2-297
1977-11-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/37/2/JV0370020297.html?itemId=/content/journal/jgv/10.1099/0022-1317-37-2-297&mimeType=html&fmt=ahah

References

  1. Blue W. T., Plummer G. 1973; Antigenic relationships among four herpesviruses. Infection and Immunity 7:1000–1002
    [Google Scholar]
  2. Cassai E., Sarmiento M. E., Spear P. G. 1975; Comparison of the virion polypeptides specified by herpes simplex virus types 1 and 2. Journal of Virology 16:1327–1331
    [Google Scholar]
  3. Cassai E., Manservigi R., Corrallini A., Terni M. 1976; Plaque dissociation of herpes simplex viruses; biochemical and biological characteristics of the viral variants. Intervirology 6:212–223
    [Google Scholar]
  4. Castrucci G., Pedim B., Cilli V., Arancia G. 1972; Characterization of a viral agent resembling bovine herpes mammillitis virus. Veterinary Record 90:325–335
    [Google Scholar]
  5. De Ley J. 1969; Compositional nucleotide distribution and the theoretical prediction of homology in bacterial DNA. Journal of Theoretical Biology 22:89–116
    [Google Scholar]
  6. Dolyniuk M., Pritchett R., Kieff E. D. 1976a; Proteins of Epstein-Barr Virus I. Analysis of the polypeptides of purified enveloped Epstein-Barr virus. Journal of Virology 17:935–949
    [Google Scholar]
  7. Dolyniuk M., Wolff E., Kieff E. D. 1976b; Proteins of Epstein-Barr virus II. Electrophoretic analysis of the polypeptides of the nucleocapsid and the glucosamine and polysaccharide-containing components of enveloped virus. Journal of Virology 18:289–297
    [Google Scholar]
  8. Fenner F. 1976; Classification and nomenclature of viruses. Second report of the International Committee on Taxonomy of Viruses. Intervirology 7:1–115
    [Google Scholar]
  9. Fiala M., Honess R. W., Heiner D. C., Heine J. W., Murnane J., Wallace R., Guze L. B. 1976; Cytomegalovirus proteins. I. Polypeptides of virions and dense bodies. Journal of Virology 19:243–254
    [Google Scholar]
  10. Gibson W., Roizman B. 1972; Proteins specified by herpes simplex virus. VIII. Characterization and composition of multiple capsid forms of subtypes 1 and 2. Journal of Virology 10:1044–1052
    [Google Scholar]
  11. Gibson W., Roizman B. 1974; Proteins of herpes simplex virus. X. Staining and radiolabeling properties of B capsid and virion proteins in polyacrylamide gels. Journal of Virology 13:155–165
    [Google Scholar]
  12. Goodheart C., Plummer G. 1975; The densities of herpesviral DNAs. Progress in Medical Virology 19:324–352
    [Google Scholar]
  13. Gupta P., St Jeor S., Rapp F. 1977; Comparison of the polypeptides of several strains of human cytomegalovirus. Journal of General Virology 34:447–454
    [Google Scholar]
  14. Hampar B., Martos L. M. 1973; Immunological relationships. In The Herpesviruses chapter 7 pp 221–259 Edited by Kaplan A. S. London and New York: Academic Press;
    [Google Scholar]
  15. Heine J. W., Honess R. W., Cassai E., Roizman B. 1974; Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. Journal of Virology 14:640–651
    [Google Scholar]
  16. Honess R. W., Powell K. L., Robinson D. J., Sim C., Watson D. H. 1974; Type specific and type common antigens in cells infected with herpes simplex virus type 1 and on the surfaces of naked and enveloped particles of the virus. Journal of General Virology 22:159–169
    [Google Scholar]
  17. Honess R. W., Watson D. H. 1977; Unity and diversity in the herpesviruses. Journal of General Virology 37:15–38
    [Google Scholar]
  18. Kieff E., Joyer B., Bachenheimer S., Roizman B. 1972; Genetic relatedness of type 1 and type 2 herpes simplex viruses. Journal of Virology 9:738–745
    [Google Scholar]
  19. Kim K. S., Sapienza V. J., Carp R. I., Moon H. M. 1976a; Analysis of structural proteins of purified murine cytomegalovirus. Journal of Virology 17:906–915
    [Google Scholar]
  20. Kim K. S., Sapienza V. J., Carp R. I., Moon H. M. 1976b; Analysis of structure proteins of purified human cytomegalovirus. Journal of Virology 20:604–611
    [Google Scholar]
  21. Kirkwood J., Geering G., Old L. 1972; Demonstration of group- and type-specific antigens of herpesviruses. In Oncogenesis and Herpesviruses pp 479 Edited by Biggs P. M., de The G., Payne L. N. Lyons: IARC;
    [Google Scholar]
  22. Lowry O. H., Rosenbrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  23. Ludwig H. O., Biswal N., Benyesh-Melnick M. 1972; Studies on the relatedness of herpesviruses through DNA-DNA hybridization. Virology 49:95–101
    [Google Scholar]
  24. Martin W. B., Hay D., Crawford L. V., Le Bovier G. L., Crawford E. M. 1966; Characteristics of bovine mammillitis virus. Journal of General Microbiology 45:325–332
    [Google Scholar]
  25. Mayr A., Bohm H. C., Brill J., Woychiechowskas S. 1965; Characterisierum eines Stutenabortvirus aus Polen und Vergleich Mit bekannten Rhinopneumonitisvirusstammen des Pferdes. Archiv fur die gesamte Virusforchung 17:216–230
    [Google Scholar]
  26. O’Callaghan D. J., Randall C. C. 1976; Molecular anatomy of herpesviruses: recent studies. Progress in Medical Virology 22:152–210
    [Google Scholar]
  27. Perdue M. L., Kemp M. C., Randall C. C., O’Callaghan D. J. 1974; Studies on the molecular anatomy of the L-M strain of equine herpes virus type 1: Proteins of the nucleocapsid and intact virion. Virology 59:201–216
    [Google Scholar]
  28. Pereira L., Cassai E., Honess R. W., Roizman B., Terni M., Nahmias A. J. 1976; Variability in the structural polypeptides of herpes simplex virus i strains: Potential application in molecular epidemiology. Infection and Immunity 13:211–220
    [Google Scholar]
  29. Powell K. L., Watson D. H. 1975; Some structural antigens of herpes simplex virus type 1. Journal of General Virology 29:167–178
    [Google Scholar]
  30. Roizman B., Furlong D. 1974; The replication of herpesviruses. In Comprehensive Virology vol 3 pp 229–403 Edited by Wagner R. R., Fraenkel-Conrat H. New York and London: Plenum Press;
    [Google Scholar]
  31. Ross L. J. N., Frazier J. A., Biggs P. M. 1972; An antigen common to some avian and mammalian herpesviruses. In Oncogenesis and Herpes Viruses pp 480–484 Edited by Biggs P. N., de The G., Payne L. N. Lyons: IARC;
    [Google Scholar]
  32. Russell W. C. 1962; A sensitive and precise plaque assay for herpes virus. Nature, London 195:1028–1029
    [Google Scholar]
  33. Sabin A. B. 1934; Studies on the B Virus. I. The immunological identity of a virus isolated from a human case of ascending myelitis associated with visceral necrosis. British Journal of Experimental Pathology 15:248–268
    [Google Scholar]
  34. Sarov I., Abady I. 1975; The morphogenesis of human cytomegalovirus: isolation and polypeptide characterization of cytomegalovirions and dense bodies. Virology 66:464–473
    [Google Scholar]
  35. Shimono H., Ben-Porat T., Kaplan A. S. 1969; Synthesis of proteins in cells infected with herpesvirus. I. Structural viral proteins. Virology 37:49–55
    [Google Scholar]
  36. Sim C., Watson D. H. 1973; The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex types 1 and 2. Journal of General Virology 19:217–233
    [Google Scholar]
  37. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. Journal of Virology 9:143–159
    [Google Scholar]
  38. Sterz H., Ludwig H., Rott R. 1973/4; Immunologic and genetic relationship between herpes simplex virus and bovine herpes mammillitis virus. Intervirology 2:1–13
    [Google Scholar]
  39. Stevely W. S. 1975; Virus-induced proteins in pseudorabies-infected cells. II. Proteins of the virion and nucleocapsid. Journal of Virology 16:944–950
    [Google Scholar]
  40. Thomson G. R., Mumford J. A., Campbell J., Griffiths L., Clapham P. 1976; Serological detection of equid herpesvirus i infections of the respiratory tract. British Equine Veterinary Journal 8:58–65
    [Google Scholar]
  41. Watson D. H., Russell W. C., Wildy P. 1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250–260
    [Google Scholar]
  42. Watson D. H., Shedden W. I. H., Elliot A., Tesuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399–408
    [Google Scholar]
  43. Watson D. H., Wildy P., Harvey B. A. M., Shedden W. I. H. 1967; Serological relationships among viruses of the herpes group. Journal of General Virology 1:139–141
    [Google Scholar]
  44. Watson D. H., Wildy P. 1969; The preparation of ‘monoprecipitin’ antisera to herpes virus specific antigens. Journal of General Virology 4:163–168
    [Google Scholar]
  45. Wildy P. 1972; Classification and nomenclature of viruses. First report of the International Committee on Nomenclature of Viruses. In Monographs in Virology vol 5 Basel: Karger;
    [Google Scholar]
  46. Wildy P. 1973; Herpes: history and classification. In The Herpesviruses chapter 1 pp 1–25 Edited by Kaplan A. S. New York and London: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-2-297
Loading
/content/journal/jgv/10.1099/0022-1317-37-2-297
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error