1887

Abstract

Summary

The mol. wt. of the three double-stranded RNA (ds-RNA) components of virus S have been re-determined by using polyacrylamide gel electrophoresis (a) of native ds-RNA with ds-RNA standards and (b) of denatured ds-RNA with single-stranded RNA standards. Good agreement between the two methods and confirmation that the relationship between log mol. wt. and electrophoretic mobility of ds-RNA is not linear were obtained. The new mol. wt. values (4·0 to 4·2 × 10; 2·5 to 2·7 × 10 and 0·26 to 0·28 × 10, respectively) are significantly higher than those determined previously by linear extrapolation from reovirus ds-RNA standards.

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/content/journal/jgv/10.1099/0022-1317-37-1-215
1977-10-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-1-215
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