1887

Abstract

Summary

The mol. wt. of the three double-stranded RNA (ds-RNA) components of virus S have been re-determined by using polyacrylamide gel electrophoresis (a) of native ds-RNA with ds-RNA standards and (b) of denatured ds-RNA with single-stranded RNA standards. Good agreement between the two methods and confirmation that the relationship between log mol. wt. and electrophoretic mobility of ds-RNA is not linear were obtained. The new mol. wt. values (4.0 to 4.2 × 10; 2.5 to 2.7 × 10 and 0.26 to 0.28 × 10, respectively) are significantly higher than those determined previously by linear extrapolation from reovirus ds-RNA standards.

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1977-10-01
2022-01-28
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