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Circular and circular-linear DNA molecules of herpes simplex virus were isolated from infected cells. Two types of circular-linear DNA molecules are described, one with a circular component of about 8 µm and a linear component of 45 µm and the other with a circular component of 45 µm and a linear component of 8 µm. The circular DNA molecules were either the size of linear DNA molecules or were shorter and corresponded to the length of the L (long) component of linear virus DNA.
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