1887

Abstract

Summary

Cells were cultured from the breast muscle of 11- to 12-day-old chick embryos and were grown under conditions optimal for the development of the cells into terminally differentiated, fused myotubes. Myotubes were infected with influenza virus A/Ann Arbor/6/60(H2N2) at high multiplicity, and synthesis of virus-specific proteins and RNAs was detected by haemadsorption, fluorescence microscopy and/or isotope labelling and electrophoresis techniques. Provided that myotubes were maintained at temperatures below 39 °C after infection, production of virus components and yield of infectious virus in these cells was similar to those observed in infected chick kidney cells. However, if cells were maintained at temperatures of 39 ° to 40 °C after infection, virus nucleoprotein was prominent in the nuclei, and synthesis of virus-specific polypeptides and of plus-strand RNA was reduced about fourfold to 20-fold compared to that detected at lower temperatures. Moreover, infectious virus was not produced when temperatures of 39 to 40 °C were used during virus replication. The results demonstrate that under suitable conditions avian myotubes formed in culture resemble epithelioid cells in their ability to support the productive replication of influenza virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-1-161
1977-10-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/37/1/JV0370010161.html?itemId=/content/journal/jgv/10.1099/0022-1317-37-1-161&mimeType=html&fmt=ahah

References

  1. Appleyard O., Maber H. B. 1974; Plaque formation by influenza viruses in the presence of trypsin. Journal of General Virology 25:351–357
    [Google Scholar]
  2. Barry R. D. 1964; The effects of actinomycin D and ultraviolet irradiation on the production of fowl plague virus. Virology 24:563–569
    [Google Scholar]
  3. Choppin P. W. 1962; Plaque formation by influenza A2 virus in monkey kidney cells. Virology 18:332–334
    [Google Scholar]
  4. Cox N. J., Kendal A. P. 1976; Presence of a segmented single-stranded RNA genome in influenza C virus. Virology 74:239–241
    [Google Scholar]
  5. Dowdle W. R., Galphin J. C., Coleman M. T., Schild G. C. 1974; A simple double immunodiffusion test for typing influenza viruses. Bulletin of the World Health Organization 51:213–218
    [Google Scholar]
  6. Eaton B. T., Hapel A. J. 1976; Persistent noncytolytic togavirus infection of primary mouse muscle cells. Virology 72:266–271
    [Google Scholar]
  7. Franklin R. M., Breitenfeld P. M. 1959; The abortive infection of Earle’s L-cells by fowl plague virus. Virology 8:293–307
    [Google Scholar]
  8. Goldberg R. S., Crowell R. L. 1971; Susceptibility of differentiating muscle cells of the fetal mouse in culture to Coxsackievirus A13. Journal of Virology 6:759–769
    [Google Scholar]
  9. Hildebrandt H. M., Maassab H. F., Willis P. W. 1962; Influenza virus pericarditis. American Journal of Diseases of Children 104:579–582
    [Google Scholar]
  10. Hills W. D., Moffat M. A., Holtermann O. A. 1960; The development of soluble (S) and viral (V) antigens of influenza A virus in tissue culture as studied by the fluorescent antibody technique. 3. Studies on the abortive cycle of replication in He La cells. Acta Pathologica et Microbiologica Scandinavica 50:419–429
    [Google Scholar]
  11. Kaighn M. E., Ebert J. D., Stott P. M. 1968; The susceptibility of differentiating muscle clones to Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 56:133–149
    [Google Scholar]
  12. Kendal A. P., Galphin J. P., Palmer E. L. 1977a; Replication of influenza virus at elevated temperatures: production of virus-like particles with reduced matrix protein content. Virology 76:186–195
    [Google Scholar]
  13. Kendal A. P., Cox N. J., Murphy B. R., Spring S. B., Maassab H. F. 1977b; Comparative studies of wild-type and cold-mutant (temperature-sensitive) influenza viruses: geneology of the matrix (M) and nonstructural (NS) proteins in recombinant cold-adapted H3N2 viruses. Journal of General Virology 37:145–159
    [Google Scholar]
  14. Laemmli V. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Lash J. W., Holtzer H., Swift H. 1957; Regeneration of mature skeletal muscle. Anatomical Record 128:679–687
    [Google Scholar]
  16. Lee H. H., Kaighn M. E., Ebert J. D. 1968; Induction of thymidine-3H incorporation in multinucleated myotubes by Rous sarcoma virus. International Journal of Cancer 3:126–136
    [Google Scholar]
  17. Lehmann-Grube F. 1965; Influenza viruses in cell cultures. 3. Propagation in calf kidney cells. Archiv für die gesamte Virusforschung 17:534–548
    [Google Scholar]
  18. Maassab H. F. 1959; The propagation of multiple viruses in chick kidney cultures. Proceedings of the National Academy of Sciences of the United States of America 45:1035–1039
    [Google Scholar]
  19. Mogabgab W. J., Holmes B., Pelon W. 1961; Influenza A and B viruses in monkey kidney cultures. I. Properties of the viruses. Journal of Infectious Diseases 108:315–323
    [Google Scholar]
  20. Molinaro M., Zani B., Martinozzi M., Monesi V. 1974; Selective effects of actinomycin D on myosin biosynthesis and myoblast fusion during myogenesis in culture. Experimental Cell Research 88:403–405
    [Google Scholar]
  21. Nuss D. L., Oppermann H., Koch G. 1975; Selective blockage of initiation of host protein synthesis in RNA-virus-infected cells. Proceedings of the National Academy of Sciences of the United States of America 72:1258–1262
    [Google Scholar]
  22. Okazaki K., Holtzer H. 1966; Myogenesis: fusion, myosin synthesis and the mitotic cycle. Proceedings of the National Academy of Sciences of the United States of America 56:1484–1490
    [Google Scholar]
  23. O’Neill M. C., Kendal A. P. 1975; Infection of differentiating muscle cells with influenza and Newcastle disease viruses. Nature, London 253:195–198
    [Google Scholar]
  24. O’Neill M. C., Stockdale F. E. 1972; A kinetic analysis of myogenesis in vitro. Journal of Cell Biology 52:52–65
    [Google Scholar]
  25. Oseasohn R., Adelson L., Kaji M. 1959; Clinicopathologic study of thirty-three fatal cases of Asian influenza. New England Journal of Medicine 260:509–518
    [Google Scholar]
  26. Partin J. C., Schubert W. K., Partin J. S., Jacobs R., Saalfeld K. 1976; Isolation of influenza virus from liver and muscle biopsy specimens from a surviving case of Reye’s Syndrome. Lancet599–602
    [Google Scholar]
  27. Pons M. W. 1973; The inhibition of influenza virus RNA by actinomycin D and cycloheximide. Virology 47:823–832
    [Google Scholar]
  28. Scholtissek C., Rott R. 1970; Synthesis in vivo of influenza virus plus and minus strand RNA and its preferential inhibition by antibiotics. Virology 40:989–996
    [Google Scholar]
  29. Stockdale F. E., Holtzer H. 1961; DNA synthesis and myogenesis. Experimental Cell Research 24:508–520
    [Google Scholar]
  30. Stockdale F. E., O’Neill M. C. 1972a; DNA synthesis, mitosis and skeletal muscle differentiation. In Vitro 8:212–227
    [Google Scholar]
  31. Stockdale F. E., O’Neill M. C. 1972b; Repair DNA synthesis in differentiating embryonic muscle cells. Journal of Cell Biology 52:589–597
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-1-161
Loading
/content/journal/jgv/10.1099/0022-1317-37-1-161
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error