1887

Abstract

SUMMARY

Reverse transcriptase activity was detected in the supernatants of rat embryo fibroblast cell cultures transformed by HSV types 1 and 2 at either the sub-optimal temperature of 20 °C or the supra-optimal temperature of 42 °C. Rat cell clones which had been transformed at 20 °C contained higher levels of C-type virus DNA polymerase than did cell clones which had been transformed at 42 °C. Syncytia formation typical for C-type RNA viruses occurred at passages higher than 24. The activation of endogenous C-type RNA viruses was independent of the virus and transformation method used.

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/content/journal/jgv/10.1099/0022-1317-36-2-365
1977-08-01
2021-10-19
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References

  1. Aaronson S. A., Stephenson J. R. 1976; Endogenous type-C RNA viruses of mammalian cells. Biochimica et Biophysica Acta 458:323–354
    [Google Scholar]
  2. Baltimore D., Smoler D. 1971; Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 68:1507–1511
    [Google Scholar]
  3. Darai G., Munk K. 1973; Human embryonic lung cells abortively infected with herpes virus hominis type 2 show some properties of cell transformation. Nature New Biology 241:268–269
    [Google Scholar]
  4. Darai G., Munk K. 1976; Neoplastic transformation of rat embryo cells with herpes simplex virus. International Journal of Cancer 18:469–481
    [Google Scholar]
  5. Darai G., Braun R., Flugel R. M., Munk K. 1977; Malignant transformation of rat embryo fibroblasts by HSV types 1 and 2 at sub-optimal temperature. Nature, London 265:744–745
    [Google Scholar]
  6. Duff R., Rapp F. 1971; Properties of hamster embryonic fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2. Journal of Virology 8:469–477
    [Google Scholar]
  7. Gross L., Schidlovsky G., Feldman D., Dreyfuss Y., Moore L. A. 1975; C-type virus particles in placenta of normal healthy Sprague-Dawley rats. Proceedings of the National Academy of Sciences of the United States of America 72:3240–3244
    [Google Scholar]
  8. Flam R. G. 1965; Clonal growth of mammalian cells in a chemically defined synthetic medium. Proceedings of the National Academy of Sciences of the United States of America 53:288–293
    [Google Scholar]
  9. Hampar B., Aaronson S. A., Derge J. G., Chakrabarty M., Show-Alter S. D., Dunn C. Y. 1976; Activation of an endogenous mouse type C virus by ultraviolet-irradiated herpes simplex virus types I and 2. Proceedings of the National Academy of Sciences of the United States of America 73:646–650
    [Google Scholar]
  10. Klement V., Rowe W. P., Hartley J. W., Pugh W. E. 1969; Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proceedings of the National Academy of Sciences of the United States of America 63:753–758
    [Google Scholar]
  11. Li J. L. H., Jerkofsky M. A., Rapp F. 1975; Demonstration of oncogenic potential of mammalian cells transformed by DNA-containing viruses following photodynamic inactivation. International Journal of Cancer 15:190–202
    [Google Scholar]
  12. Mcdougall J. K. 1970; Effects of adenoviruses on the chromosomes of normal human cells and cells trisomic for an E chromosome. Nature, London 225:456–458
    [Google Scholar]
  13. Macnab J. C. M. 1974; Transformation of rat embryo cells by temperature-sensitive mutants of herpes simplex virus. Journal of General Virology 24:143–153
    [Google Scholar]
  14. Macpherson I., Montagnier L. 1964; Agar suspension for the selective assay of cells transformed by polyoma virus. Virology 24:291–294
    [Google Scholar]
  15. Munk K., Darai G., Deinhardt F., Marczynska B., Nonoyama M., Silver S. 1975; Herpes simplex virus neoplastic transformation. Vllth International Symposium on Comparative Leukemia Research, Copenhagen. Comparative Leukemia Research Institute. Bibliotheca Haematologica 43:59–62
    [Google Scholar]
  16. Munyon W., Kraiselburd E., Davis D., Zeigel R., Buchsbaum R., Paoletti E. 1971; Biochemical transformation ofL-cells with ultraviolet-irradiated herpes simplex virus. Federation Proceedings 31:1669
    [Google Scholar]
  17. Reed C. L., Rapp F. 1976; Induction of murine P30 by superinfecting herpesviruses. Journal of Virology 19:1028–1033
    [Google Scholar]
  18. Wells R. D., Flugel R. M., Larson J. E., Schendel P. F., Sweet R. W. 1972; Comparison of some reactions catalyzed by deoxyribonucleic acid polymerase from avian myeoblastosis virus, Escherichia coli, and Micrococcus luteus. Biochemistry u:621–629
    [Google Scholar]
  19. Wu A. M., Gallo R. C. 1975; Reverse transcriptase. CRC Critical Reviews in Biochemistry 3:289–347
    [Google Scholar]
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