1887

Abstract

SUMMARY

We have shown previously that a non-fatal outcome of infection with street rabies virus occurs more often when mice are exposed to a high ambient temperature (HAT = 35 °C) early in the course of the infection. To determine what influence the virus strain had on this protective effect of HAT, we have extended these observations to studies of a fixed rabies strain, CVS and several substrains of CVS virus derived from temperature-sensitive (ts) mutants. In all cases, mortality was reduced to some extent by exposure of the animals to HAT; however, dramatic strain-specific differences in the extent of the effect were noted. Although each of the virus substrains tested was revertant in the ts character (as tested using a non-permissive temperature of 40.5 °C), several substrains (ts 1, ts 4, RT) caused disease that was sensitive (> 90% reduction in mortality) to HAT. Mortality induced by the parental CVS virus was reduced approx. 50% at HAT. A single CVS virus substrain, VSW, caused disease that was less affected by HAT than was disease induced by the parental strain. As in previous studies with street virus, the incubation periods for infection with CVS virus substrains were consistently prolonged at HAT.

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/content/journal/jgv/10.1099/0022-1317-36-2-307
1977-08-01
2021-10-17
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References

  1. Bell J. F. 1975; Latency and abortive rabies. In The Natural History of Rabies pp 331–354 Edited by Baer G. New York, San Francisco, London: Academic Press, Inc;
    [Google Scholar]
  2. Bell J. F., Moore G. J. 1974; Effects of high ambient temperature on various stages of rabies virus infection in mice. Infection and Immunity 10:510–515
    [Google Scholar]
  3. Clark H. F. 1972; Growth and attenuation of rabies virus in cell cultures of reptilian origin. Proceedings of the Society for Experimental Biology and Medicine 139:1317–1325
    [Google Scholar]
  4. Clark H. F., Koprowski H. 1971; Isolation of temperature-sensitive conditional lethal mutants of ‘fixed’ rabies virus. Journal of Virology 7:295–300
    [Google Scholar]
  5. Clark H. F., Ohtani S. 1976; Temperature-sensitive mutants of rabies virus in mice: a mutant (ts 2) revertant mixture selectively pathogenic by the peripheral route of inoculation. Infection and Immunity 13:1418–1425
    [Google Scholar]
  6. Clark H. F., Wiktor T. J. 1972; Temperature-sensitivity characteristics distinguishing substrains of fixed rabies virus: lack of correlation with plaque–size markers or virulence for mice. Journal of Infectious Diseases 125:637–646
    [Google Scholar]
  7. Colquhon D. 1971 In Lectures on Biostatistics chapter 8 pp 117–123 London: Oxford University Press;
    [Google Scholar]
  8. Crick J., Brown F. 1974; An interfering component of rabies virus which contains RNA. Journal of General Virology 22:147–151
    [Google Scholar]
  9. Haldane J. B. S. 1960; ‘Dex’ or order of magnitude. Nature, London 187:879
    [Google Scholar]
  10. Holland J. J., Villarreal L. P. 1975; Purification of defective interfering T particles of vesicular stomatitis and rabies viruses generated in vivo in brains of newborn mice. Virology 67:438–449
    [Google Scholar]
  11. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  12. Kawai A., Matsumoto S., Tanabe K. 1975; Characterization of rabies viruses recovered from persistently infected BHK cells. Virology 67:520–523
    [Google Scholar]
  13. Richman D. D., Murphy B. R., Spring S. B., Coleman M. T., Chanock R. M. 1975; Temperature sensitive mutants of influenza virus. IX. Genetic and biological characterization of TS–l[E] lesions when transferred to a 1972 (H3N2) influenza A virus. Virology 66:551–562
    [Google Scholar]
  14. Stanners C. P., Farmilo A. J., Goldberg V. J. 1975; Effects in vitro and in vivo of a mutant of vesicular stomatitis virus with attenuated cytopathogenicity. In Negative Strand Viruses pp 785–798 Edited by Mahy B. W. J., Barry R. D. New York: Academic Press, Inc;
    [Google Scholar]
  15. Wagner R. R. 1974; Pathogenicity and immunogenicity for mice of temperature–sensitive mutants of vesicular stomatitis virus. Infection and Immunity 10:309–315
    [Google Scholar]
  16. Wiktor T., Dietzschold B., Leamnson R. N., Koprowski H. 1977; Induction and biological properties of defective interfering particles of rabies virus. Journal of Virology 21:626–635
    [Google Scholar]
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