1887

Abstract

SUMMARY

In order to clucidate the properties of an inhibitor of rabies virus haemagglutinin in normal animal sera, experiments were made with the HEP Flury strain and calf serum which contains the inhibitor. The results of physico-chemical treatment, gelfiltration and density analysis suggested lipoprotein involvement. When inhibitor and haemagglutinin were mixed, the separate activities could be recovered from the mixture by centrifuging on a sucrose density gradient. By contrast, neither haemagglutinin nor inhibitor could be recovered by this treatment when the inhibitor was added at the start of virus growth. The binding of inhibitor with rabies virus during virus growth seems irreversible and different from the binding of inhibitor with pre-formed rabies haemagglutinin.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-36-1-31
1977-07-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/36/1/JV0360010031.html?itemId=/content/journal/jgv/10.1099/0022-1317-36-1-31&mimeType=html&fmt=ahah

References

  1. Clark D. H., Casals J. 1958; Techniques for hemagglutination inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  2. Furukawa T., Plotkin S., Sedwick D., Profeta M. 1967; Haemagglutination of rubella virus. Nature, London 215:172–173
    [Google Scholar]
  3. Halonen P. E., Murphy F. A., Fields B. N., Reese D. R. 1968; Hemagglutinin of rabies and some other bullet-shaped viruses. Proceedings of the Society for Experimental Biology and Medicine 127:1037–1042
    [Google Scholar]
  4. Halonen P. E., Toivanen P., Nikkari T. 1974; Non-specific serum inhibitors of activity of haemagglutinins of rabies and vesicular stomatitis viruses. Journal of General Virology 22:309–318
    [Google Scholar]
  5. Havel R. J., Eder H. A., Bragdon J. H. 1955; The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. Journal of Clinical Investigation 34:1345–1353
    [Google Scholar]
  6. Kondo A. 1965; Growth characteristics of rabies virus in primary chick embryo cells. Virology 27:199–204
    [Google Scholar]
  7. Kondo A., Takashima Y., Suzuki M. 1972; Inactivated rabies vaccine of chick embryo cell culture origin. International Symposium on Rabies (2), Lyon. In Symposia Series in Immunobiological Standardization vol 21 pp 182–189 Basel: Karger;
    [Google Scholar]
  8. Kuwert E., Wiktor T. J., Sokol F., Koprowski H. 1968; Hemagglutination by rabies virus. Journal of Virology 2:1381–1392
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-36-1-31
Loading
/content/journal/jgv/10.1099/0022-1317-36-1-31
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error