1887

Abstract

Summary

Human papovavirus, RFV, isolated from urine of a renal transplant patient was compared with two strains of SV40 and with the prototype human papovavirus, BKV. Neutralization tests showed that RFV and BKV are indistinguishable, while large-plaque (LP) and small-plaque (SP) isolates of SV40 gave a low but significant level of cross-reaction with rabbit or human antisera against RFV.

DNA reassociation saturation tests using I-labelled RFV DNA show that BKV has 88% homology, and SP-SV40 has 29% homology to RFV. We conclude that RFV and BKV are nearly, if not totally, identical and are not SV40 variants.

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/content/journal/jgv/10.1099/0022-1317-35-1-67
1977-04-01
2024-04-26
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References

  1. Britten R. J., Graham D. L., Neufeld B. R. 1974; Analysis of repeating DNA sequences by reassociation. In Methods in Enzymology vol 29 pp. 363–418 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  2. Britten R. J., Kohne D. E. 1967; Nucleotide sequence repetition in DNA. Carnegie Institution of Washington Yearbook 65:78–106
    [Google Scholar]
  3. Dougherty R. M., DisTefano H. S. 1974; Isolation and characterization of a papovavirus from human urine. Proceedings of the Society for Experimental Biology and Medicine 146:481–487
    [Google Scholar]
  4. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science 130:432–437
    [Google Scholar]
  5. Gardner S. D., Field A. M., Coleman D. V., Hulme B. 1971; New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet i:1253–1257
    [Google Scholar]
  6. Gelb L., Kohne D. E., Martin M. 1971; Quantitation of simian virus 40 sequences in African green monkey, mouse and virus-transformed cell lines. Journal of Molecular Biology 57:129–145
    [Google Scholar]
  7. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  8. Howley P. M., Khoury G., Byrne J. C., Takemoto K. K., Martin M. A. 1975a; Physical map of the BK virus genome. Journal of Virology 16:959–973
    [Google Scholar]
  9. Howley P. M., Mullarkey M. F., Takemoto K., Martin M. 1975b; Characterization of human papovavirus BK DNA. Journal of Virology 15:173–181
    [Google Scholar]
  10. Major E. O., Dimayorca G. 1973; Malignant transformation of BHK21 clone 13 cells by BK virus - a human papovavirus. Proceedings of the National Academy of Sciences of the United States of America 70:3210–3212
    [Google Scholar]
  11. Miao R., Dougherty R. M. 1976; Characterization of human papovavirus RFV: use of iodinated viral DNA to detect viral DNA sequences in cellular DNA. Virology (in the press)
    [Google Scholar]
  12. Padgett B. L., Walker D. L., Zurhein G. M., Eckroade R. J., Dessell B. H. 1971; Cultivation of papovaa-like virus from human brain with progressive multifocal leukoencephalopathy. Lancet 1:1257–1260
    [Google Scholar]
  13. Penney J. B., Narayan O. 1973; Studies of the antigenic relationship of the new human papovaviruses by electron microscopy agglutination. Infection and Immunity 8:299–300
    [Google Scholar]
  14. Portolani M., Barbanti-Brodano G., Laplaca M. 1975; Malignant transformation of hamster kidney cells by BK virus. Journal of Virology 15:420–422
    [Google Scholar]
  15. Shah K. V., Daniel R. W., Strandberg J. D. 1975; Sarcoma in a hamster inoculated with BK virus, a human papovavirus. Journal of the National Cancer Institute 54:945–949
    [Google Scholar]
  16. Sharp P. A., Pettersson U., Sambrook J. 1974; Viral DNA in transformed cells. I. A study of the sequences of adenovirus-2 DNA in a line of transformed rat cells using specific fragments of the viral genome. Journal of Molecular Biology 86:709–726
    [Google Scholar]
  17. Takemoto K. K., Mullarkey M. F. 1973; Human papovavirus, BK strain. Biological studies including antigenic relationship to simian virus 40. Journal of Virology 12:625–631
    [Google Scholar]
  18. Weiner L. P., Herndon R. M., Narayan O., Johnson R. T. 1972a; Further studies of a simian virus 40-like virus isolated from human brain. Journal of Virology 10:147–149
    [Google Scholar]
  19. Weiner L. P., Herndon R. M., Narayan O., Johnson R. T., Shah K., Rubinstein L. J., Preziosi T. J., Conley F. K. 1972b; Isolation of virus related to SV4., from patients with progressive multifocal leukoencephalopathy. New England Journal of Medicine 286:385–390
    [Google Scholar]
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