The extinction-temperature profile of parsley carrot-leaf virus in 0.02 -(Na-K) phosphate buffer, pH 7.2, containing 0.1 -NaCl, was determined. At the T (dissociation temperature = 65 °C), the point at which begins to increase, the virus particles apparently dissociate to form RNA and empty protein shells (top component). At the T (temperature at the inflexion point of the curve = 70 °C), corresponding to half the maximum increase in , the protein denatures and precipitates. Temperatures below T have little effect on the virus, whereas temperatures higher than T also degrade the RNA. The results of the present work seem to suggest that the increase in of PCLV preparations upon heating is mostly attributable to turbidity caused by coat protein denaturation rather than to effects on RNA.


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