The synthesis of proteins has been studied in tobacco protoplasts infected with cowpea chlorotic mottle virus (CCMV). Three new proteins were found with mol. wt. 19000 (P1), 34000 to 36000 (P2) and 100000 (P3). Protein P2 reached a maximum steady level about 16 to 20 h after infection; it was not a precursor of P1, which is virus coat protein. P3 appeared later than the other two proteins, reached its maximum about 44 h after infection and then declined. P2 and P3 were localized in the 1000 and 20000 pellets of a cell homogenate, in contrast to P1 (coat) which was largely found in the supernatant fraction after centrifuging at 20000 for 30 min. The three proteins account for about 60% of the tripartite genome of CCMV.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error