1887

Abstract

SUMMARY

Methods are described for the preparation and authentication of a highly specific antiserum against herpesvirus saimiri (HVS) capsid antigens. The antiserum was used in immunofluorescence tests to follow the development of capsid antigens in HVS-infected owl monkey kidney cells throughout the virus replication cycle in parallel with sequential titrations of virus infectivity in both cells and medium. Fluorescence was detected as a round or oval, bright green area of staining at the centre of the nucleus which was similar in outline to the Cowdry type A inclusion seen in HVS-infected cells stained by haematoxylin and eosin. The first detection of fluorescence towards the end of the eclipse phase of the virus growth cycle, and its abolition by the treatment of infected cultures with cytosine arabinoside confirmed the identity of HVS capsid antigens as late antigens. The failure to detect fluorescence in the cytoplasm of HVS-infected cells has brought to light a conflict between the site of accumulation of virus capsid antigens as determined by immunofluorescence and the finding, by electron microscopy, of cytoplasmic immature particles in intact cells during the early stages of the virus replication cycle. The significance of this discrepancy is discussed in relation to its possible existence for other members of the herpesvirus group.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-34-1-61
1977-01-01
2024-05-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/34/1/JV034001061.html?itemId=/content/journal/jgv/10.1099/0022-1317-34-1-61&mimeType=html&fmt=ahah

References

  1. Albrecht P., Blaskovic D., Jakubik J., Lesso J. 1963; Demonstration of pseudorabies virus in chick embryo cell cultures and infected animals by the fluorescent antibody technique. Acta Virologica 7:289–296
    [Google Scholar]
  2. Crowle A. J. 1958; A simplified micro double-diffusion agar precipitin technique. Journal of Laboratory Clinical Medicine 52:784–787
    [Google Scholar]
  3. Daniel M. D., Rabin H., Barahona II. H., Meléndez I. V. 1971; Plaque formation under multi agar, methyl cellulose and starch overlays. Proceedings of the Society for Experimental Biology and Medicine 136:1192–1196
    [Google Scholar]
  4. Elson C. J., Singh J., Taylor R. B. 1973; The effect of capping by anti-immunoglobulin antibody on the expression of cell surface immunoglobulin and on lymphocyte activation. Scandinavian Journal of Immunology 3:143–149
    [Google Scholar]
  5. Epstein M. A. 1962; Observations on the mode of release of herpes virus from infected HeLa cells. Journal of Cell Biology 12:589–597
    [Google Scholar]
  6. Epstein M. A., Achong B. G., Barr Y. M. 1964; Virus particles in cultured lymphoblasts from Burkitt’s lymphoma. Lancet 1:702–703
    [Google Scholar]
  7. Epstein M. A., Holt S. J. 1963; Electron microscopic observations on the surface adenosine triphosphatase-like enzymes of HeLa cells infected with herpes virus. Journal of Cell Biology 19:337–347
    [Google Scholar]
  8. Fujiwara S., Kaplan A. S. 1967; Site of protein synthesis in ceils infected with pseudorabies virus. Virology 32:60–68
    [Google Scholar]
  9. Géder L., Váczi L., Jeney E., Gönczöl E., Lehel F. 1967; ‘Early non-virion’ antigen in herpes simplex infected tissue culture cells. II. Intracellular localization of ‘early’ antigen with indirect immunofluorescence technique. Acta Microbiologica Academiae Scientarum Hungaricae 13:365–372
    [Google Scholar]
  10. Géder L., Váczi L. 1968; Localization of nuclear and cytoplasmic herpes simplex antigens in infected cells by immunofluorescence. Acta Virologica 12:97–105
    [Google Scholar]
  11. Habel K. 1969; Virus neutralization test. In Fundamental Techniques in Virology pp 288–296 Edited by Habel K., Salzman N. P. New York: Academic Press;
    [Google Scholar]
  12. Kaplan A. S. 1957; A study of the herpes simplex-rabbit kidney cell system by the plaque technique. Virology 4:435–457
    [Google Scholar]
  13. Klein G., Pearson G., Rabson A., Ablashi D. V., Falk L., Wolfe L., Deinhardt F., Rabin H. 1973; Antibody reactions to Herpesvirus saimiri (HVS)-induced early and late antigens (EA and LA) in HVS-infected squirrel, marmoset and owl monkeys. International Journal of Cancer 12:270–289
    [Google Scholar]
  14. Meléndez L. V., Daniel M. D., Garcia F. G., Fraser C. E. O., Hunt R. D, King N. W. 1969a; Herpesvirus saimiri. I. Further characterization studies of a new virus from the squirrel monkey. Laboratory Animal Care 19:372–377
    [Google Scholar]
  15. Meléndez L. V., Hunt R. D., Daniel M. D., Garcia F. G., Fraser C. E. O. 1969b; Herpesvirus Saimiri. II. Experimentally induced malignant lymphoma in primates. Laboratory Animal Care 19:378–386
    [Google Scholar]
  16. Morgan D. G., Achong B. G., Epstein M. A. 1973; Unusual intranuclear tubular structures associated with the maturation of Herpesvirus saimiri in monkey kidney cells. British Journal of Cancer 27:434–440
    [Google Scholar]
  17. Morgan D. G., Achong B. G., Epstein M. A. 1976; Morphological observations on the replication of herpesvirus saimiri in monkey kidney cell cultures. Journal of General Virology 32:461–470
    [Google Scholar]
  18. Morgan D. G., Epstein M. A., Achong B. G., Meléndez L. V. 1970; Morphological confirmation of the herpes nature of a carcinogenic virus of primates (Herpes saimiri). Nature, London 228:170–172
    [Google Scholar]
  19. Neurath A. R., Rubin B. A. 1971; Viral structural components as immunogens of prophylactic value. In Monographs in Virology vol 4 pp 3–7 Edited by Melnick J. L. Basel, Switzerland: S. Karger;
    [Google Scholar]
  20. Olshevsky U., Levitt J., Becker Y. 1967; Studies on the synthesis of herpes simplex virions. Virology 33:323–334
    [Google Scholar]
  21. Powell K. L., Buchan A., Sim C., Watson D. H. 1974; Type-specific protein in herpes simplex envelope reacts with neutralising antibody. Nature, London 249:360–361
    [Google Scholar]
  22. Powell K. L., Watson D. H. 1975; Some structural antigens of herpes simplex virus type 1. Journal of General Virology 29:167–178
    [Google Scholar]
  23. Roane P. R., Roizman B. 1966; Differentiation of nuclear and cytoplasmic herpesvirus antigens in infected cells. Virology 29:668–670
    [Google Scholar]
  24. Robinson D. J., Watson D. H. 1971; Structural proteins of herpes simplex virus. Journal of General Virology 10:163–171
    [Google Scholar]
  25. Roizman B. 1969; The herpesviruses - a biochemical definition of the group. In Current Topics in Microbiology and Immunology vol 49 pp 1–79 Berlin: Springer-Verlag;
    [Google Scholar]
  26. Roizman B., Spring S. B., Roane P. R. 1967; Cellular compartmentalization of herpesivrus antigens during viral replication. Journal of Virology 1:181–192
    [Google Scholar]
  27. Ross L. J. N., Watson D. H., Wildy P. 1968; Development and localization of virus-specific antigens during the multiplication of herpes simplex virus in BHK-21 cells. Journal of General Virology 2:115–122
    [Google Scholar]
  28. Spear P. G., Roizman B. 1968; The proteins specified by herpes simplex virus. 1. Time of synthesis, transfer into nuclei, and properties of proteins made in productively infected cells. Virology 36:545–555
    [Google Scholar]
  29. Sydiskis R. J., Roizman B. 1966; Polysomes and protein synthesis in cells infected with a DNA virus. Science 153:76–78
    [Google Scholar]
  30. Williams C. A., Chase M. W. 1971 In Methods in Immunology and Immunochemistry vol 3 pp 234–273 Edited by Williams C. A., Chase M. W. New York; Academic Press:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-34-1-61
Loading
/content/journal/jgv/10.1099/0022-1317-34-1-61
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error