1887

Abstract

SUMMARY

An investigation of the activity of nuclear RNA polymerase following infection of LS cells with HSV-1 shows a decline in both major activities. This effect is not entirely due to inhibition of cellular protein synthesis, and the effect of α-amanitin-sensitive RNA polymerase is mediated by a protein(s) synthesized in the infected cell. Changes in the properties of this RNA polymerase activity include a reduction in the relative UTP/GTP incorporation ratio and an increased sensitivity to inhibition by actinomycin D, indicating that RNA polymerase II is involved in virus transcription.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-33-3-471
1976-12-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/33/3/JV0330030471.html?itemId=/content/journal/jgv/10.1099/0022-1317-33-3-471&mimeType=html&fmt=ahah

References

  1. Abrahams J. C., Hay J. 1972; Effect of pseudorabies virus on the metabolism of low-molecular-weight ribonucleic acid in BHK-C13 cells. Biochemical Journal 129:39P–40P
    [Google Scholar]
  2. Alwine J. C., Steinhart W. L., Hill C. W. 1974; Transcription of herpes simplexvirus type 1 DNA in nuclei isolated from infected HEp-2 and KB cells. Virology 60:302–307
    [Google Scholar]
  3. Bayliss O. J., Marsden H. S., Hay J. 1975; Herpes simplexvirus proteins: DNA-binding proteins in infected cells and in virus structure. Virology 68:124–134
    [Google Scholar]
  4. Blatti S. V., Ingles C. J., Lindell X. J., Morris P. W., Weaver R. F., Weinberg F., Rotter W. J. 1970; Structure and regulatory properties of eukaryotic RNA polymerase. Cold Spring Harbor Symposium on Quantitative Biology 35:649–657
    [Google Scholar]
  5. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–323
    [Google Scholar]
  6. Chandler J. K., Stevely W. S. 1973; Virus-induced proteins in pseudorabies-infected cells. 1. Acid-extractable proteins of the nucleus. Journal of Virology 11:815–822
    [Google Scholar]
  7. Cochet-meilhac M., Chambon P. 1974; Animal DNA-dependent RNA polymerases. II. Mechanism of the inhibition of RNA polymerases B by amatoxins. Biochimica et Biophysica Acta 353:160–184
    [Google Scholar]
  8. Flamm W. G., Bond H. E., Burr H. E. 1966; Density-gradient centrifugation of DNA in a fixed-angle rotor: a high order of resolution. Biochimica et Biophysica Acta 129:310–319
    [Google Scholar]
  9. Flanagan J. F. 1967; Virus-specified ribonucleic acid synthesis in KB cells infected with herpes simplexvirus. Journal of Virology 1:583–590
    [Google Scholar]
  10. Graham F. L., Veldhuisen G., Wilkie N. M. 1973; Infectious herpesvirus DNA. Nature New Biology 245:265–266
    [Google Scholar]
  11. Hay J., Koteles G. J., Keir H. M., Subak-Sharpe J. H. 1966; Herpesvirus specified ribonucleic acids. Nature, Lotidon 210:387–390
    [Google Scholar]
  12. Jacob S. T. 1973; Mammalian RNA polymerases. Progress in Nucleic Acid Research and Molecular Biology 13:93–126
    [Google Scholar]
  13. McCormick F. P., Newton A. A. 1975; Polyamine metabolism in cells infected with herpes simplexvirus. Journal of General Virology 27:25–33
    [Google Scholar]
  14. Mcreynolds L., Penman S. 1974; A polymerase activity forming 5S and pre-4S RNA in isolated HeLa cell nuclei. Cell 1:139–145
    [Google Scholar]
  15. Morrison J. M., Keir H. M. 1968; A new DNA exonuclease in cells infected with herpes virus: partial purification and properties of the enzyme. Journal of General Virology 3:337–347
    [Google Scholar]
  16. Perry R. P., Kelley D. E. 1970; Inhibition of RNA synthesis by actinomycin D: characteristic dose-response of different RNA species. Journal of Cellular Physiology 76:127–139
    [Google Scholar]
  17. Plagemann P. G. W. 1971a; Nucleotide pools of Novikoff rat hepatoma cells growing in suspension culture. 1. Kinetics of incorporation of nucleotide pools and pool sizes during growth cycle. Journal of Cellular Physiology 77:213–240
    [Google Scholar]
  18. Plagemann P. G. W. 1971b; Nucleotide pools of Novikoff rat hepatoma cells growing in suspension culture. 2. Independent nucleotide pools for nucleic acid synthesis. Journal of Cellular Physiology 77:241–258
    [Google Scholar]
  19. Pogo A. O., Littau V. C., Allfrey V. G., Mirsky A. E. 1967; Modification of ribonucleic acid synthesis in nuclei isolated from normal and regenerating liver: some effects of salt and specific divalent cations. Proceedings of the National Academy of Sciences of the United States of America 57:743–750
    [Google Scholar]
  20. Preston C. M. 1975; Studies on RNA synthesis in cultured mammalian cells. Ph.D. thesis University of Cambridge;
    [Google Scholar]
  21. Rakusanova T., Ben-porat T., Himeno M., Kaplan A. S. 1971; Early functions of the genome of herpesvirus. I. Characterisation of the RNA synthesised in cycloheximide-treated infected cells. Virology 46:877–889
    [Google Scholar]
  22. Rakusanova T., Ben-porat T., Kaplan A. S. 1972; Effects of herpesvirus infection on the synthesis of cell-specific RNA. Virology 49:537–548
    [Google Scholar]
  23. Randareth K., Randareth E. 1967; Thin-layer separation methods for nucleic acid derivatives. Methods in Enzymology 12A:323–347
    [Google Scholar]
  24. Reeder R. H., Roeder R. G. 1972; Ribosomal RNA synthesis in isolated nuclei. Journal of Molecular Biology 67:433–441
    [Google Scholar]
  25. Roeder R. G., Rutter W. J. 1970; Specific nucleolar and nucleoplasmic RNA polymerases. Proceedings of the National Academy of Sciences of the United States of America 65:675–682
    [Google Scholar]
  26. Roizman B., Frenkel N. 1973; The transcription and state of herpes simplexvirus DNA in productive infection and in human cervical cancer tissue. Cancer Research 33:1402–1416
    [Google Scholar]
  27. Sasaki Y., Sasaki R., Cohen G. H., Pizer L. I. 1974; RNA polymerase activity and inhibition on herpesvirus-infected cells. Intervirology 3:148–161
    [Google Scholar]
  28. Sydiskis R. J., Roizman B. 1967; The disaggregation of host polyribosomes in productive and abortive infection with herpes simplexvirus. Virology 32:678–686
    [Google Scholar]
  29. Thompson R. J. 1973; Studies on RNA synthesis in two populations of nuclei from the mammalian cerebral cortex. Journal of Neurochemistry 21:19–40
    [Google Scholar]
  30. Wagner E. K., Roizman B. 1969a; Ribonucleic acid synthesis in cells infected with herpes simplexvirus.1. Patterns of ribonucleic acid synthesis in productively infected cells. Journal of Virology 4:36–46
    [Google Scholar]
  31. Wagner E. K., Roizman B. 1969b; RNA synthesis in cells infected with herpes simplexvirus. 2. Evidence that a class of viral mRNA is derived from a high molecular weight precursor synthesised in the nucleus. Proceedings of the National Academy of Sciences of the United States of America 64:626–633
    [Google Scholar]
  32. Wagner E. K., Swanstrom R. L., Stafford M. G. 1972; Transcription of the herpes simplexvirus genome in human cells. Virology 10:675–682
    [Google Scholar]
  33. Weinmann R., Roeder R. G. 1974; Role of DNA-dependent RNA polymerase. III. In the transcription of the tRNA and 5S RNA genes. Proceedings of the National Academy of Sciences of the United States of America 71:1790–1794
    [Google Scholar]
  34. Wtdnell C. C., Tata J. F. 1964; Evidence for two DNA-dependent RNA polymerase activities in isolated rat-liver nuclei. Biochimica et Biophysica Acta 87:53–533
    [Google Scholar]
  35. Zylber E. A., Penman S. 1971; Products of RNA polymerases in HeLa cell nuclei. Proceedings of the National Academy of Sciences of the United States of America 68:2861–2865
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-33-3-471
Loading
/content/journal/jgv/10.1099/0022-1317-33-3-471
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error