The number of infective centres which were established successfully following the manual inoculation of French bean leaves with tobacco necrosis virus strain D (TNV) or with TNV RNA, decreased with increasing temperature between 13 and 30 °C. At 30 °C or above, primary and probably also secondary infections could not be established, though it is likely that a limited amount of virus RNA and nucleoprotein was produced at 30 °C in cells in which infection had been established previously at 23 °C. During the first day after inoculation, 23 °C was optimal for virus accumulation. Between 23 and 30 °C the rate at which lesions increased in diameter decreased with increasing temperature. The inhibitory effect of supraoptimal temperatures on the establishment of infection may be due to degradation of the infective entity by ribonuclease(s).


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