1887

Abstract

SUMMARY

The use of enzyme-linked antibodies for the detection of two morphologically different plant viruses is described. The technique is extremely sensitive, enabling assay of the viruses at concentrations as low as 10 to 100 ng/ml both in purified preparations and in crude plant extracts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-33-1-165
1976-10-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/33/1/JV0330010165.html?itemId=/content/journal/jgv/10.1099/0022-1317-33-1-165&mimeType=html&fmt=ahah

References

  1. Avrameas S. 1969; Coupling of enzymes to proteins with gluteraldehyde. Immunochemistry 6:43–52
    [Google Scholar]
  2. Engvall E., Perlmann P. 1971; Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8:871–874
    [Google Scholar]
  3. Engvall E., Perlmann P. 1972; Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 109:129–135
    [Google Scholar]
  4. Voller A., Bidwell D., Huldt G., Engvall E. 1974; A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bulletin of the World Health Organization 51:209–211
    [Google Scholar]
  5. Voller A., Bidwell D. E., Bartlett A. 1976; Enzyme immunoassays in diagnostic medicine: theory and practice. Bulletin of the World Health Organization (in the press)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-33-1-165
Loading
/content/journal/jgv/10.1099/0022-1317-33-1-165
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error