1887

Abstract

SUMMARY

A circular dichroism comparative study of isolated and phage R17 RNA reveals in both cases the same degree of base pairing. However, thermal circular dichroism melting profiles exhibit the presence of free energy of interaction between RNA and capsid protein. It is apparent that the capsid stabilizes the RNA structure with and without the addition of Mg. A close RNA capsid association is also derived from pH titration circular dichroism studies. The pH melting of the RNA starts to occur about 0.5 pH unit higher with and without the addition of Mg than the acid denaturation of isolated RNA. A direct correlation between bathochromic CD peak shift of the main position band and loss of survivors is noted for the thermal melting as well as pH titration experiments. It is suggested that the heat and pH induced conformational alterations of R17 RNA coinciding with loss of infectivity occur after an alteration of nucleic acid-capsid protein interaction.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-32-2-177
1976-08-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/32/2/JV0320020177.html?itemId=/content/journal/jgv/10.1099/0022-1317-32-2-177&mimeType=html&fmt=ahah

References

  1. ADAMS M. H.1959Bacteriophages50New York: Interscience Publishers, Inc
    [Google Scholar]
  2. ADOLPH K. W.1975Conformational features of cowpea chlorotic mottle virus RNA and the stability of the virusJournal of General Virology28:137–145
    [Google Scholar]
  3. BOBST A. M., PAN Y. E., PHILLIPS D. J.1974Comparative optical property studies on polycistronic R17 phage RNA and rabbit globin mRNABiochemistry13:2129–2133
    [Google Scholar]
  4. BOEDTKER H.1968Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehydeJournal of Molecular Biology35:61–70
    [Google Scholar]
  5. BRAHMS J., MOMMAERTS W. F. H. M.1964A study of conformation of nucleic acids in solutions by means of circular dichroismJournal of Molecular Biology10:73–88
    [Google Scholar]
  6. CANTOR C. R., JASKUNAS S. R., JUN. TINOCO I.1966Optical properties of ribonucleic acids predicted from oligomersJournal of Molecular Biology20:39–62
    [Google Scholar]
  7. GESTELAND R. F., BOEDTKER H.1964Some physical properties of bacteriophage R17 and its ribonucleic acidJournal of Molecular Biology8:496–507
    [Google Scholar]
  8. GESTELAND R. F., SPAHR P. F.1970A new procedure for the preparation of highly labeled intact [sup]32[/sup]P RNA from bacteriophage R17Biochemical and Biophysical Research Communications41:1267–1272
    [Google Scholar]
  9. HARTMAN K. A., CLAYTON N., JUN. THOMAS G.1973Studies of virus structure by Raman spectroscopy, R17 virus and R17 RNABiochemical and Biophysical Research Communications50:942–949
    [Google Scholar]
  10. HENKENS R. W., MIDDLEBROOK J. L.1973Optical and hydrodynamic studies of the structure of bacteriophage f2Biochemistry12:2910–2915
    [Google Scholar]
  11. ISENBERG H., COTTER R. I., GRATZER W. B.1971Secondary structure and interaction of RNA and protein in a bacteriophageBiochimica et Biophysica Acta 23z,184–191
    [Google Scholar]
  12. KAPER J. M.1969Nucleic acid-protein interactions in turnip yellow mosaic virusScience, New York166:248–250
    [Google Scholar]
  13. KOZAK M., NATHANS D.1971Fate of maturation protein during infection by coliphage MS 2Nature New Biology234:209–211
    [Google Scholar]
  14. PARANCHYCH W., AINSWORTH S. K., DICK A. J., KRAHN P. M.1971Stages in phage R17 infection. V. Phage eclipse and the role of F. piliVirology45:615–628
    [Google Scholar]
  15. PHILLIPS D. J., BOBST A. M.1972Circular dichroism melting studies on R17 phage RNABiochemiealand Biophysical Research Communications47:150–156
    [Google Scholar]
  16. REMSEN J. F., MULLER N., CERUTTI P. A.1970Photohydration of uridine in the RNA of coliphage R17. II. The relationship between ultraviolet inactivation and uridine photohydrationProceedings of the National Academy of Sciences of the United States of America65:460–466
    [Google Scholar]
  17. ROBERTS J. W., STEITZ J. A.1967The reconstitution of infective bacteriophage R17Proceedings of the National Academy of Sciences of the United States of America58:1416–1421
    [Google Scholar]
  18. SAMUELSON G., KAESBERG P.1970An artificial top component of R17 bacteriophageJournal of Molecular Biology47:87–91
    [Google Scholar]
  19. SCHUBERT D.1969Conformational changes during reversible depolymerization of the protein coat from bacteriophage frBiochimica et Biophysica Acta188:147–154
    [Google Scholar]
  20. SLEGERS H., FIERS W.1973Studies on the bacteriophage MS2. XXII. Conformation of MS 2 RNA in acid mediumBiopolymers12:2007–2021
    [Google Scholar]
  21. STEITZ J. A.1968aIdentification of the A protein as a structural component of bacteriophage R17Journal of Molecular Biology33:923–936
    [Google Scholar]
  22. STEITZ J. A.1968bIsolation of the A protein from bacteriophage R17Journal of Molecular Biology33:937–946
    [Google Scholar]
  23. STEITZ J. A.1968cA slowly sedimenting, infective form of bacteriophage R17Journal of Molecular Biology33:947–951
    [Google Scholar]
  24. TURCHINSKY M. F., KUSOVA K. S., BUDOWSKY E.I.1973Conversion of non-covalent interactions in nucleoproteins into covalent bonds: bisulfide-induced formation of polynucleotides-protein crosslinks in MS 2 bacteriophage virionsFEBS Letters38:304–307
    [Google Scholar]
  25. VERBRAEKEN E., FIERS W.1972aFurther evidence on the role of the A protein in bacteriophage MS 2 particlesFEBS Letters28:89–92
    [Google Scholar]
  26. VERBRAEKEN E., FIERS W.1972bStudies on the bacteriophage MS 2. XX. Expansion of the virion in low saltVirology50:690–700
    [Google Scholar]
  27. WARSHAW M. M., JUN. TINOCO I.1966Optical properties of sixteen dinucleoside phosphatesJournal of Molecular Biology20:29–38
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-32-2-177
Loading
/content/journal/jgv/10.1099/0022-1317-32-2-177
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error