1887

Abstract

SUMMARY

/r ( ) was infected, at 30 °C, with T4D , T4D ( , , DNA-negative phenotype), and T4D ( , , maturation-defective phenotype). A genetic (‘transformation’) assay was used to monitor transcription of genes (polynucleotide ligase), (deoxycytidylate hydroxymethylase), (DNA polymerase), , and (endolysin). The principal results are: (1) All of the genes studied were transcribed exclusively from the so-called -strand of phage DNA. (2) DNA synthesis and the maturation-defective proteins were required to turn-off transcription of genes , and . Experiments performed with chloramphenicol suggested that all phage-specific proteins required to turn-off transcription of these genes were not present until 6 to 8 min post infection (p.i.). (3) During a normal developmental programme, gene was transcribed throughout the eclipse. DNA-negative and maturation-defective conditions had no obvious effect on transcription of this gene. (4) During a normal lytic event, two discrete waves of gene transcription were observed. The late wave was dependent upon DNA-synthesis and presence of functional maturation-defective proteins. The early wave was unaffected by DNA-negative or maturation-defective conditions. Experiments with chloramphenicol indicated that, if any virus-specific proteins are involved with regulation of early transcription, such proteins are present by 3 min p.i.

The data are interpreted to mean that early gene transcription is regulated by a minimum of two mechanisms. One of these mechanisms is fully operational by the 3rd min and, among the genes studied, controlled early transcription. A second mechanism becomes operational between 6 and 8 min p.i. and controls transcription of genes , and .

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1976-06-01
2022-01-16
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References

  1. ANDO T. 1966; A nuclease specific for heat-denatured DNA isolated from a product. of Aspergillus oryzae. Biochimica et Biophysica Acta 114:158–168
    [Google Scholar]
  2. BAUTZ E. K. F., BAUTZ F. A. 1970; Studies on the function of the RNA polymerase σ factor in promoter selection. Cold Spring Harbor Symposium on Quantitative Biology 35:227–232
    [Google Scholar]
  3. BAUTZ E. K. F., KASAI T., REILLY E., BAUTZ F. A. 1966; Gene-specific mRNAII. Regulation of mRNA synthesis in E. coli after infection with bacteriophage T4. Proceedings of the National Academy of Sciences of the United States of America 55:1081–1088
    [Google Scholar]
  4. BERNARDI G., BERNARDI A. 1966 Procedures in Nucleic Acid Research144–153 Edited by Cantoni G. L., Davies D. R. New York: Harper and Row;
    [Google Scholar]
  5. BOLLE A., EPSTEIN R. H., SALSER W., GEIDUSCHEK E. P. 1968a; Transcription during bacteriophage T4 development: synthesis and relative stability of early and late RNA. Journal of Molecular Biology 31:325–348
    [Google Scholar]
  6. BOLLE A., EPSTEIN R. H., SALSER W., GEIDUSCHEK E. P. 1968b; Transcription during bacteriophage T4 development: requirements for late messenger synthesis. Journal of Molecular Biology 33:339–362
    [Google Scholar]
  7. BOLUND C. 1973; Influence of gene 55 on the regulation of some early enzymes of bacteriophage T4-infected Escherichia coli. Journal of Virology 12:49–57
    [Google Scholar]
  8. BOLUND C., SKÖLD O. 1971; Formation of RNA in T4 phage-infected bacteria: transcriptional regulation of early enzyme synthesis. Virology 43:390–402
    [Google Scholar]
  9. BOLUND C., SKÖLD O. 1973; Regulation of early RNA synthesis in bacteriophage T4-infected bacteria: dependence on bacteriophage-specific protein synthesis. Journal of Virology 12:39–48
    [Google Scholar]
  10. BRODY E. N., DIGGELMANN H., GEIDUSCHEK E. P. 1970a; Transcription of the bacteriophage T4 template. Obligate synthesis of T4 prereplicative RNA. in vitro. Biochemistry 9:1289–1299
    [Google Scholar]
  11. BRODY E. N., SEDEROFF R., BOLLE A., EPSTEIN R. H. 1970b; Early transcription in T4-infected cells. Cold Spring Harbor Symposium on Quantitative Biology 35:203–211
    [Google Scholar]
  12. EPSTEIN R. H., BOLLE A., STEINBERG C. M., KELLENBERGER E., BOY DELATOUR E., CHEVALLEY R., EDGAR R. S., SUSMAN R., DENHARDT G. H., LIELAUSIS A. 1963; Physiological studies of conditional lethal mutants of bacteriophage T4D. Cold Spring Harbor Symposium on Quantitative Biology 28:375–394
    [Google Scholar]
  13. GEIDUSCHEK E. P., SNYDER L., COLVILL A. J. E., SARNAT M. 1966; Selective synthesis of T-even bacteriophage early messenger in vitro. Journal of Molecular Biology 19:541–547
    [Google Scholar]
  14. GUHA A., SZYBALSKI W. 1968; Fractionation of the complementary strands of coliphage T4 DNA based on the asymmetric distribution of the Poly U and Poly U,G binding sites. Virology 34:608–616
    [Google Scholar]
  15. GUHA A., SZYBALSKI W., SALSER W., BOLLE A., GEIDUSCHEK E. P., PULITZER J. 1971; Controls and polarity of transcription during bacteriophage T4 development. Journal of Molecular Biology 59:329–349
    [Google Scholar]
  16. JAYARAMAN R. 1972; Transcription of bacteriophage DNA by Escherichia coli RNA polymerase in vitro: identification of some immediate early and delayed early genes. Journal of Molecular Biology 70:253–263
    [Google Scholar]
  17. JAYARAMAN R., GOLDBERG E. B. 1969; A genetic assay for mRNA’s of phage T4. Proceedings of the National Academy of Sciences of the United States of America 64:198–204
    [Google Scholar]
  18. JAYARAMAN R., GOLDBERG E. B. 1970; Transcription of the bacteriophage T4 genome in vivo. Cold Spring Harbor Symposium on Quantitative Biology 35:197–201
    [Google Scholar]
  19. KASAI T., BAUTZ E. K. F. 1967 Organizational Macromolecules111–123 Edited by Bogel H. J., Lampen J. O., Bryson E. New York: Academic Press;
    [Google Scholar]
  20. KASAI T., BAUTZ E. K. F. 1969; Regulation of gene specific RNA synthesis in bacteriophage T4. Journal of Molecular Biology 41:401–417
    [Google Scholar]
  21. LEMBACH K., KUNINAKA A., BUCHANAN J. M. 1969; The relationship of DNA replication to the control of protein synthesis in protoplasts of T4-infected Escherichia coli B. Proceedings of the National Academy of Sciences of the United States of America 62:446–453
    [Google Scholar]
  22. NOTANI G. W. 1973; Regulation of bacteriophage T4 gene expression. Journal of Molecular Biology 73:231–249
    [Google Scholar]
  23. PULITZER J. F. 1970a; Function of T4 gene 55. I. Characterization of temperature-sensitive mutations in the ‘maturation’ gene 55. Journal of Molecular Biology 49:473–488
    [Google Scholar]
  24. PULITZER J. F. 1970b; Function of T4 gene 55II. RNA synthesis by temperature-sensitive gene 55 mutants. Journal of Molecular Biology 49:489–507
    [Google Scholar]
  25. RIVA S., CASCINO A., GEIDUSCHEK E. P. 1970; Coupling of late transcription to DNA replication in bacteriophage T4 development. Journal of Molecular Biology 54:85–102
    [Google Scholar]
  26. SAKIYAMA S., BUCHANAN J. M. 1972; Control of the synthesis of T4 phage deoxynucleotide kinase messenger ribonucleic acid in vivo. Journal of Biological Chemistry 247:7806–7814
    [Google Scholar]
  27. SALSER W., BOLLE A., EPSTEIN R. H. 1970; Transcription during bacteriophage T4 development: a demonstration that distinct subclasses of ‘early’ RNA appear at different times and that some are ‘turned-off’ at late times. Journal of Molecular Biology 49:281–295
    [Google Scholar]
  28. SCHMIDT D. A., MAZAITIS A. J., KASAI T., BAUTZ E. K. F. 1970; Involvement of phage T4 σ factor and an anti-terminator protein in the transcription of early T4 genes in vivo. Nature, London 225:1012–1016
    [Google Scholar]
  29. SIPPEL A., HARTMANN G. 1968; Mode of action of rifamycin on the RNA polymerase reaction. Biochimica et Biophysica Acta 157:218–219
    [Google Scholar]
  30. SUTTON W. D. 1971; A crude nuclease preparation suitable for use in DNA reassociation experiments. Biochimica et Biophysica Acta 240:522–531
    [Google Scholar]
  31. WAIS H. C., GOLDBERG E. B. 1969; Growth and transformation of phage T4 in Escherichia coli B/4, Salmonella, Aerobacter, Proteus, and Serratia. Virology 39:153–161
    [Google Scholar]
  32. WARNER H. R., HOBBS M. D. 1968; Nucleotide accumulations in Escherichia coli infected with some bacteriophage T4 amber mutants. Virology 36:527–537
    [Google Scholar]
  33. WEHRLI W., KNÜSEL F., STAEHELIN M. 1968a; Action of rifamycin on RNA polymerase from sensitive and resistant bacteria. Biochemical and Biophysical Research Communications 32:284–288
    [Google Scholar]
  34. WEHRLI W., NÜESCH J., KNÜSEL F., STAEHELIN H. 1968b; Action of rifamycins on RNA polymerase. Biochimica et Biophysica Acta 157:215–217
    [Google Scholar]
  35. WILHELM J. A., HASELKORN R. 1971; In vitro synthesis of T4 proteins: the products of genes 9, 18, 19, 23, 24, and 38. Virology 43:198–208
    [Google Scholar]
  36. WITMER H. J. 1971; In vitro transcription of T4 deoxyribonucleic acid by Escherichia coli ribonucleic acid polymerase: sequential transcription of immediate early and delayed early cistrons in the absence of the release factor, rho. Journal of Biological Chemistry 246:5220–5227
    [Google Scholar]
  37. WITMER H. J. 1974; In vivo transcription of baseplate genes 9,10, and 11 of bacteriophage T4. Biochimica et Biophysica Acta 335:424–431
    [Google Scholar]
  38. WITMER H. J. 1975; Effect of DNA-negative and maturation-defective conditions on accumulation of functional messengers for T4 bacteriophage-specific dihydrofolate reductase and deoxynucleoside monophosphate kinase. Journal of Virology 15:1511–1513
    [Google Scholar]
  39. YOUNG E. T. 1970; Control of functional T4 messenger synthesis. Cold Spring Harbor Symposium on Quantitative Biology 35:189–195
    [Google Scholar]
  40. YOUNG E. T., VAN HOWE G. 1970; Control of synthesis of glucosyltransferase and lysozyme messenger after T4 infection. Journal of Molecular Biology 51:605–619
    [Google Scholar]
  41. ZWEERINK H., GOLDBERG E. B. 1970; Transformation of phage T4 by small denatured DNA fragments. Virology 41:175–178
    [Google Scholar]
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