1887

Abstract

SUMMARY

About 80% of the RNA molecules extracted from encephalomyocarditis (EMC) virus were bound by oligo(dT)-cellulose under conditions which bind poly(A) but not poly(C) nor ribosomal RNA. This shows that most EMC virus RNA molecules contain a poly(A) tract. Both bound and unbound fractions contained RNA molecules of apparently the same size when examined by sucrose gradient sedimentation, but the bound fraction contained an adenylic acid-rich segment of about 20 nucleotides long, whereas the unbound RNA did not. The bound RNA had 200 times the specific infectivity of the unbound RNA which suggests that the poly(A) tract present in EMC virus RNA is required for infectivity.

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1976-05-01
2024-04-15
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References

  1. AVIV H., LEDER P. 1972; Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proceedings of the National Academy of Sciences of the United States of America 69:1408–1412
    [Google Scholar]
  2. BRAWERMAN G. 1974; Eukaryotic messenger RNA. Annual Review of Biochemistry 43:621–642
    [Google Scholar]
  3. BURNESS A. T. H. 1969; Purification of encephalomyocarditis virus. Journal of General Virology 5:291–303
    [Google Scholar]
  4. BURNESS A. T. H. 1970; Ribonucleic acid content of encephalomyocarditis virus. Journal of General Virology 6:373–380
    [Google Scholar]
  5. BURNESS A. T. H., CLOTHIER F. W. 1970; Particle weight and other biophysical properties of encephalomyocarditis virus. Journal of General Virology 6:381–393
    [Google Scholar]
  6. BURNESS A. T. H., FOX S. M., PARDOE I. U. 1974; The polypeptide composition of the encephalomyocarditis virus particle. Journal of General Virology 23:225–236
    [Google Scholar]
  7. BURNESS A. T. H., PARDOE I. U., GOLDSTEIN N. O. 1975; Overestimates of the size of poly(A) segments. Biochemical and Biophysical Research Communications 67:1408–1414
    [Google Scholar]
  8. EATON B. T., FAULKNER P. 1972; Heterogeneity in the poly(A) content of the genome of Sindbis virus. Virology 50:865–873
    [Google Scholar]
  9. FENWICK M. L. 1968; The effect of reaction with formaldehyde on the sedimentation rates of ribonucleic acids. Biochemical Journal 107:851–859
    [Google Scholar]
  10. HOSKINS J. M., SANDERS F. K. 1957; Propagation of mouse encephalomyocarditis virus in ascites tumour cells maintained in vitro. British Journal of Experimental Pathology 38:268–269
    [Google Scholar]
  11. MILLER R. L., PLAGEMANN P. G. W. 1972; Purification of Mengovirus and identification of an A-rich segment in its ribonucleic acid. Journal of General Virology 17:349–353
    [Google Scholar]
  12. MONTAGNIER L., SANDERS F. K. 1962; Titrage de l’acide ribonucléique infectieux du virus de l’encéphalo-myocardite de la souris sur cellules d’ascite Krebs II. in vitro. Compte Rendu Hebdomadaires des Seances de l’Académie des Sciences (Paris 354:2247–2249
    [Google Scholar]
  13. NEWMAN J. F. E., BROWN F. 1976; Absence of poly(A) from the infective RNA of Nodamura virus. Journal of General Virology 30:137–140
    [Google Scholar]
  14. PERRY R. P., LA TORRE J., KELLY D. F., GREENBERG J. R. 1972; On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes. Biochimica et Biophysica Acta 262:220–226
    [Google Scholar]
  15. PORTER A., CAREY N., FELLNER P. 1974; Presence of a large poly(rC) tract within the RNA of encephalo-myocarditis virus. Nature, London 248:675–678
    [Google Scholar]
  16. SANDERS F. K. 1964 The infective nucleic acids of animal viruses. Techniques in Experimental Virology277–304 Edited by Harris R. S. C. New York: Academic Press, Inc;
    [Google Scholar]
  17. SANDERS F. K., HUPPERT J., HOSKINS J. M. 1958; Replication of an animal virus. Symposia of the Society for Experimental Biology 12:123–137
    [Google Scholar]
  18. SHATKIN A. J. 1974; Animal RNA viruses: genome structure and function. Annual Review of Biochemistry 43:643–665
    [Google Scholar]
  19. SPECTOR D. H., BALTIMORE D. 1974; Requirement of 3′-terminal poly (adenylic acid) for the infectivity of poliovirus RNA. Proceedings of the National Academy of Sciences of the United States of America 71:2983–2987
    [Google Scholar]
  20. SPECTOR D. H., BALTIMORE D. 1975; Poly(A) in Mengovirus RNA. Journal of Virology 16:1081–1084
    [Google Scholar]
  21. STUDY GROUP ON PICORNAVIRIDAE 1975; Picornaviridae. Intervirology 4:303–316
    [Google Scholar]
  22. WILD T. F., BROWN F. 1970; Replication of foot-and-mouth disease virus ribonucleic acid. Journal of General Virology 7:1–11
    [Google Scholar]
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