1887

Abstract

SUMMARY

The negative staining-carbon technique has been applied to suspensions of freshly prepared type strain tobacco mosaic virus particles in high concentrations. Electron microscope images show that paracrystalline arrays of the virus were formed, in which large areas of rods could be viewed along their long axes and in parallel arrays in horizontal positions. High-resolution micrographs showed the protein structure units in rods photographed in both vertical and horizontal orientations.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-2-265
1976-05-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/2/JV0310020265.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-2-265&mimeType=html&fmt=ahah

References

  1. BEESTON B. E. P., HORNE R. W., MARKHAM R. 1973 Electron Diffraction and Optical Diffraction Techniques: Practical Methods in Electron Microscopy Edited by Glauert Audrey M. Amsterdam: North-Holland;
    [Google Scholar]
  2. CASPAR D. L. D. 1963; Assembly and stability of the tobacco mosaic virus particle. Advances in Protein Chemistry 18:37–121
    [Google Scholar]
  3. FINCH J. T. 1964; Resolution of the substructure of tobacco mosaic virus in the electron microscope. Journal of Molecular Biology 8:872–874
    [Google Scholar]
  4. FINCH J. T. 1972; The hand of the helix of tobacco mosaic virus. Journal of Molecular Biology 66:291–294
    [Google Scholar]
  5. FINCH J. T., HOLMES K. C. 1967 Structural studies of viruses. Methods in Virology III351 Edited by Maramorosch K., Koprowski H. New York and London: Academic Press;
    [Google Scholar]
  6. GOODMAN R. M., HORNE R. W., HOBART J. M. 1975a; Reconstitution of potato virus X in vitro. Characterization of the reconstituted product. Virology 68:299–303
    [Google Scholar]
  7. GOODMAN R. M., MACDONALD J. O., HORNE R. W., BANCROFT J. B. 1975b; Assembly of flexuous plant viruses and other proteins. Philosophical Transactions of the Royal Society, London B: in the press
    [Google Scholar]
  8. HART R. G., YOSHIYAMA J. M. 1975; Electron microscopy with reduced beam damage to the specimen: a retractable image intensifier. Journal of Ultrastructure Research 51:40–45
    [Google Scholar]
  9. HORNE R. W., PASQUALI-RONCHETTI I. 1974; A negative stain-carbon film technique for studying viruses in the electron microscope. I. Preparative procedures for examining icosahedral and filamentous viruses. Journal of Ultrastructure Research 47:361–383
    [Google Scholar]
  10. HORNE R. W., PASQUALI-RONCHETTI I., HOBART J. M. 1975a; A negative stain-carbon film technique for studying viruses in the electron microscope. II. Application to adenovirus type 5. Journal of Ultrastructure Research 51:233–252
    [Google Scholar]
  11. HORNE R. W., HOBART J. M., PASQUALI-RONCHETTI I. 1975b; Application of the negative stain-carbon technique to the study of virus particles and their components by electron microscopy. Micron 5:233–261
    [Google Scholar]
  12. KLUG A., BERGER J. E. 1964; An optical method for the analysis of periodicities in electron micrographs, and some observations on the mechanism of negative staining. Journal of Molecular Biology 10:564–569
    [Google Scholar]
  13. KLUG A., DE ROSIER D. J. 1966; Optical filtering of electron micrographs: Reconstruction of one-sided images. Nature, London 212:29–32
    [Google Scholar]
  14. MARKHAM R., HITCHBORN J. H., HILLS G. J., FREY S. 1964; The anatomy of the tobacco mosaic virus. Virology 22:342–359
    [Google Scholar]
  15. UNWIN P. N. T., HENDERSON R. 1975; Molecular structure determination by electron microscopy of unstained crystalline specimens. Journal of Molecular Biology 94:425–440
    [Google Scholar]
  16. WILLIAMS R. C., FISHER H. W. 1970; Electron microscopy of tobacco mosaic virus under conditions of minimal beam exposure. Journal of Molecular Biology 52:121–123
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-31-2-265
Loading
/content/journal/jgv/10.1099/0022-1317-31-2-265
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error