1887

Abstract

SUMMARY

Internal proteins, synthesized in T2-infected cells were recovered from bacterial membranes during the early stages of infection. Approx. 15 min after the onset of infection, T2 and T4 internal proteins were released from the bacterial membranes and sedimented along with newly synthesized phage DNA. Internal protein-DNA complexes were also obtained by chromatography on hydroxylapatite columns. Internal proteins were not released from bacterial membranes after infection with amber mutants defective in genes 21, 22 and 23. It has been suggested that these complexes are intermediates in T-even phage morphogenesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-1-21
1976-04-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/1/JV0310010021.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-1-21&mimeType=html&fmt=ahah

References

  1. ABRAMS A., MACNAMARA P., JOHNSON F. B. 1960; Adenosine triphosphatase in isolated bacterial cell membranes. Journal of Biological Chemistry 235:3659–3662
    [Google Scholar]
  2. ADAMS M. H. 1959 Bacteriophages New York: Interscience Publishers Inc;
    [Google Scholar]
  3. BACHRACH U., BENCHETRIT L. 1974a; Studies on phage internal proteins. II. Cleavage of a precursor of internal proteins during the morphogenesis of bacteriophage T4. Virology 59:51–58
    [Google Scholar]
  4. BACHRACH U., BENCHETRIT L. 1974b; Studies on phage internal proteins. III. Specific binding of T4 internal proteins to T4 DNA. Virology 59:443–454
    [Google Scholar]
  5. BACHRACH U., BENCHETRIT L. 1976; Cleavage of T4-induced proteins during phage morphogenesis: characterization of peptides. Journal of General Virology in the press
    [Google Scholar]
  6. BACHRACH U., FRIEDMANN A., LEVIN R., NYGAARD A. P. 1974; Transfer of internal proteins from phage to host and their association with bacterial membranes. Journal of General Virology 23:117–127
    [Google Scholar]
  7. BACHRACH U., LEVIN R., FRIEDMANN A. 1970; Studies on phage internal proteins: isolation of protein-DNA complexes from T2 phages and from phage-infected bacteria. Virology 40:882–892
    [Google Scholar]
  8. BACHRACH U., LEVIN R., SAMUM A. 1974; Specific effect of T2 internal proteins on the viscosity of T2 DNA. Biochemical and Biophysical Research Communications 60:801–806
    [Google Scholar]
  9. BLACK L. W. 1974; Bacteriophage T4 internal protein mutants: isolation and properties. Virology 60:166–179
    [Google Scholar]
  10. BLACK L. W., GOLD L. M. 1971; Pre-replicative development of the bacteriophage T4: RNA and protein synthesis in vivo and in vitro. Journal of Molecular Biology 60:365–388
    [Google Scholar]
  11. FAIRBANKS G. JUN., LEVINTHAL C., REEDER R. H. 1965; Analysis of “C-labeled proteins by disc electrophoresis. Biochemical and Biophysical Research Communications 20:393–399
    [Google Scholar]
  12. HAAR L. 1967; Messenger ribonucleic acid and polysomes in the membrane fraction of T2-infected. Escherichia coli. Biochimica et Biophysica Acta 145:202–204
    [Google Scholar]
  13. HUANG W. M., BUCHANAN J. M. 1974; Synergistic interaction of T4 early proteins concerned with their binding to DNA. Proceedings of the National Academy of Sciences of the United States of America 71:2226–2230
    [Google Scholar]
  14. KOHN A. 1960; Lysis of frozen and thawed cells of Escherichia coli by lysozyme, and their conversion into spheroplasts. Journal of Bacteriology 79:697–706
    [Google Scholar]
  15. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  16. LAEMMLI U. K., FAVRE M. 1973; Maturation of the head of bacteriophage T4. I. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  17. MAIZEL J. V. JUN. 1966; Acrylamide-gel electropherograms by mechanical fractionation: radioactive adenovirus proteins. Science, New York 151:988–990
    [Google Scholar]
  18. MEADE R. C., STIGLITZ R. A. 1962; Improved solvent systems for liquid scintillation counting of body fluids and tissues. International Journal of Applied Radiation and Isotopes 13:11–14
    [Google Scholar]
  19. ONORATO L., SHOWE M. K. 1975; Gene 21 protein-dependent proteolysis in vitro of purified gene 22 product of bacteriophage T4. Journal of Molecular Biology 92:395–412
    [Google Scholar]
  20. SHOWE M. K., BLACK L. W. 1973; Assembly core of bacteriophage T4: an intermediate in head formation. Nature New Biology 242:70–75
    [Google Scholar]
  21. SUIT J. C. 1962; Ribonucleic acid in a membrane fraction of Escherichia coli and its relation to cell wall synthesis. Journal of Bacteriology 84:1061–1070
    [Google Scholar]
  22. TREMBLAY G. Y., DANIELS M. J., SCHAECHTER M. 1969; Isolation of cell membrane-DNA-nascent RNA complex from bacteria. Journal of Molecular Biology 40:65–76
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-31-1-21
Loading
/content/journal/jgv/10.1099/0022-1317-31-1-21
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error