1887

Abstract

SUMMARY

Persistent states of measles virus infection have been established in HeLa cells by using Edmonston strain virus and two types of measles virus vaccine (M-VAC and Schwarz). The absolute amount of surface viral antigens expressed on these cells infected separately with the three viruses has been assessed by a newly developed method which employs [I]-labelled Fab fragments of immunoglobulin G (IgG) from immune human sera. This method was used to determine the level of viral antigenic expression on acutely infected HeLa cells harvested at a time when 95 to 100% of cells could be lysed by antiviral antibody and complement. From our data, more than 1 × 10 antibody molecules must bind to each cell infected with measles virus before complement dependent lysis can occur in a homologous test system. Persistently infected cells bind 2 to 3 times less antibody than acutely infected cells and correspondingly exhibit less susceptibility to humorally-mediated immune lysis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-3-329
1976-03-01
2024-11-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/30/3/JV0300030329.html?itemId=/content/journal/jgv/10.1099/0022-1317-30-3-329&mimeType=html&fmt=ahah

References

  1. Bather R., Furesz J., Fanok A. G., Gill S. D., Yarosh W. 1973; Long term infection of diploid African green monkey brain cells by Schwarz measles vaccine virus. Journal of General Virology 20:401–405
    [Google Scholar]
  2. Fahey J. L. 1967; Chromatographic separation of immunoglobulins. Methods in Immunology and Immuno-chemistry 1:321–332
    [Google Scholar]
  3. Feldman L. A., Raine C. S., Sheppard R. D., Bornstein M. B. 1972; Virus-host cell relationships in measles-infected cultures of central nervous tissue. Journal of Neuropathology and Experimental Neurology 31:624–638
    [Google Scholar]
  4. Franklin E. C. 1960; Structural units of human 7 S gamma globulin. Journal of Clinical Investigation 39:1933–1941
    [Google Scholar]
  5. Hayashi K., Rosenthal J., Notkins A. L. 1972; Iodine-125-labeled antibody to viral antigens: binding to the surface of virus-infected cells. Science, New York 176:516–518
    [Google Scholar]
  6. Jabbour J. T., Duenas D. A., Modlin J. 1975; Subacute sclerosing panencephalitis: clinical staging, course and frequency. In Symposium on Cellular Immunity and SSPE, Archives of Neurology (in the press)
    [Google Scholar]
  7. Joseph B. S., Cooper N. R., Oldstone M. B. A. 1975; Immunologic injury of cultured cells infected with measles virus. I. Role of IgG antibody and the alternative complement pathway. Journal of Experimental Medicine 141:761–774
    [Google Scholar]
  8. Joseph B. S., Oldstone M. B. A. 1974; Antibody-induced redistribution of measles virus antigens on the cell surface. Journal of Immunology 113:1205–1209
    [Google Scholar]
  9. Knight P., Duff R., Rapp F. 1972; Latency of human measles virus in hamster cells. Journal of Virology 10:995–1001
    [Google Scholar]
  10. McConahey P. J., Dixon F. J. 1966; A method of trace iodination of proteins for immunologic studies. International Archives of Allergy 29:185–189
    [Google Scholar]
  11. Minagawa T. 1971; Studies on the persistent infection with measles virus in HeLa cells. I. Clonal analysis of cells of carrier cultures. Japanese Journal of Microbiology 15:325–331
    [Google Scholar]
  12. Norrby E. 1967; A carrier cell line of measles virus in LU 106 cells. Archiv fur die Gesamte Virusforschung 20:215–224
    [Google Scholar]
  13. Parker J. C., Klintworth G. K., Graham D. G., Griffith J. F. 1970; Uncommon morphological features in subacute sclerosing panencephalitis. American Journal of Pathology 61:275–292
    [Google Scholar]
  14. Payne F. E., Baublis J. V., Itabashi H. H. 1969; Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. New England Journal of Medicine 281:585–589
    [Google Scholar]
  15. Raine C. S., Feldman L. A., Sheppard R. D., Bornstein M. B. 1971; Ultrastructural study of long-term measles infection in cultures of hamster dorsal-root ganglion. Journal of Virology 8:318–329
    [Google Scholar]
  16. Rosenthal J., Hayashi K., Notkins A. L. 1973; Virus antigens on the surface of infected cells: binding and elution of [125I]-labelled antivirus antibody. Journal of General Virology 18:195–199
    [Google Scholar]
  17. Rustigian R. 1966; Persistent infection of cells in culture by measles virus. I. Development and characteristics of HeLa sublines persistently infected with complete virus. Journal of Bacteriology 92:1792–1804
    [Google Scholar]
  18. Schneck S. A., Fulginiti V., Leestma J. 1967; Measles virus and panencephalitis. Lancet ii:1381–1382
    [Google Scholar]
  19. Ter Meulen V., Katz M., Muller D. 1972; Subacute sclerosing panencephalitis: A review. Current Topics in Microbiology and Immunology 57:1–38
    [Google Scholar]
  20. Webb H. E., Illavia S. J., Laurence G. D. 1971; Measles-vaccine viruses in tissue-culture of non-neuronal cells of human foetal brain. Lancet 11:4–5
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-30-3-329
Loading
/content/journal/jgv/10.1099/0022-1317-30-3-329
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error