Skip to content
1887

Abstract

SUMMARY

Persistent states of measles virus infection have been established in HeLa cells by using Edmonston strain virus and two types of measles virus vaccine (M-VAC and Schwarz). The absolute amount of surface viral antigens expressed on these cells infected separately with the three viruses has been assessed by a newly developed method which employs [I]-labelled Fab fragments of immunoglobulin G (IgG) from immune human sera. This method was used to determine the level of viral antigenic expression on acutely infected HeLa cells harvested at a time when 95 to 100% of cells could be lysed by antiviral antibody and complement. From our data, more than 1 × 10 antibody molecules must bind to each cell infected with measles virus before complement dependent lysis can occur in a homologous test system. Persistently infected cells bind 2 to 3 times less antibody than acutely infected cells and correspondingly exhibit less susceptibility to humorally-mediated immune lysis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-3-329
1976-03-01
2025-11-08

Metrics

Loading full text...

Full text loading...

References

  1. Bather R., Furesz J., Fanok A. G., Gill S. D., Yarosh W. 1973; Long term infection of diploid African green monkey brain cells by Schwarz measles vaccine virus. Journal of General Virology 20:401–405
    [Google Scholar]
  2. Fahey J. L. 1967; Chromatographic separation of immunoglobulins. Methods in Immunology and Immuno-chemistry 1:321–332
    [Google Scholar]
  3. Feldman L. A., Raine C. S., Sheppard R. D., Bornstein M. B. 1972; Virus-host cell relationships in measles-infected cultures of central nervous tissue. Journal of Neuropathology and Experimental Neurology 31:624–638
    [Google Scholar]
  4. Franklin E. C. 1960; Structural units of human 7 S gamma globulin. Journal of Clinical Investigation 39:1933–1941
    [Google Scholar]
  5. Hayashi K., Rosenthal J., Notkins A. L. 1972; Iodine-125-labeled antibody to viral antigens: binding to the surface of virus-infected cells. Science, New York 176:516–518
    [Google Scholar]
  6. Jabbour J. T., Duenas D. A., Modlin J. 1975; Subacute sclerosing panencephalitis: clinical staging, course and frequency. In Symposium on Cellular Immunity and SSPE, Archives of Neurology (in the press)
    [Google Scholar]
  7. Joseph B. S., Cooper N. R., Oldstone M. B. A. 1975; Immunologic injury of cultured cells infected with measles virus. I. Role of IgG antibody and the alternative complement pathway. Journal of Experimental Medicine 141:761–774
    [Google Scholar]
  8. Joseph B. S., Oldstone M. B. A. 1974; Antibody-induced redistribution of measles virus antigens on the cell surface. Journal of Immunology 113:1205–1209
    [Google Scholar]
  9. Knight P., Duff R., Rapp F. 1972; Latency of human measles virus in hamster cells. Journal of Virology 10:995–1001
    [Google Scholar]
  10. McConahey P. J., Dixon F. J. 1966; A method of trace iodination of proteins for immunologic studies. International Archives of Allergy 29:185–189
    [Google Scholar]
  11. Minagawa T. 1971; Studies on the persistent infection with measles virus in HeLa cells. I. Clonal analysis of cells of carrier cultures. Japanese Journal of Microbiology 15:325–331
    [Google Scholar]
  12. Norrby E. 1967; A carrier cell line of measles virus in LU 106 cells. Archiv fur die Gesamte Virusforschung 20:215–224
    [Google Scholar]
  13. Parker J. C., Klintworth G. K., Graham D. G., Griffith J. F. 1970; Uncommon morphological features in subacute sclerosing panencephalitis. American Journal of Pathology 61:275–292
    [Google Scholar]
  14. Payne F. E., Baublis J. V., Itabashi H. H. 1969; Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. New England Journal of Medicine 281:585–589
    [Google Scholar]
  15. Raine C. S., Feldman L. A., Sheppard R. D., Bornstein M. B. 1971; Ultrastructural study of long-term measles infection in cultures of hamster dorsal-root ganglion. Journal of Virology 8:318–329
    [Google Scholar]
  16. Rosenthal J., Hayashi K., Notkins A. L. 1973; Virus antigens on the surface of infected cells: binding and elution of [125I]-labelled antivirus antibody. Journal of General Virology 18:195–199
    [Google Scholar]
  17. Rustigian R. 1966; Persistent infection of cells in culture by measles virus. I. Development and characteristics of HeLa sublines persistently infected with complete virus. Journal of Bacteriology 92:1792–1804
    [Google Scholar]
  18. Schneck S. A., Fulginiti V., Leestma J. 1967; Measles virus and panencephalitis. Lancet ii:1381–1382
    [Google Scholar]
  19. Ter Meulen V., Katz M., Muller D. 1972; Subacute sclerosing panencephalitis: A review. Current Topics in Microbiology and Immunology 57:1–38
    [Google Scholar]
  20. Webb H. E., Illavia S. J., Laurence G. D. 1971; Measles-vaccine viruses in tissue-culture of non-neuronal cells of human foetal brain. Lancet 11:4–5
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-30-3-329
Loading
/content/journal/jgv/10.1099/0022-1317-30-3-329
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error