1887

Abstract

SUMMARY

Four isolates of carnation ringspot virus showed a reversible aggregation which was dependent on the temperature and concentration of their preparations. At 7 mg/ml, preparations of two of these isolates aggregated at room temperature but at the same concentration the other two isolates required higher temperatures (40 °C) for aggregation. A fifth isolate formed aggregates of 12 virus particles and linked aggregates. Particles of the two isolates with less tendency to aggregate were almost completely dissociated by 0.4% sodium dodecyl sulphate (SDS) at pH 5 and by 0.1% SDS at pH 7. At pH 7, an RNA component of mol. wt. 0.5 × 10 was released by lower concentrations of SDS than was an RNA component of 1.5 × 10. At pH 5, the sedimentation rates of unaggregated particles of the remaining three aggregating strains were unaffected by up to 15% SDS. However, treatment with 0.0075 to 0.05% SDS at pH 7 produced protein, RNA and swollen virus particles. These swollen particles were not further affected by increasing the SDS concentration to 8%.

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1976-03-01
2022-01-25
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References

  1. Bancroft J. B., Hiebert E., Rees M. W., Markham R. 1968; Properties of cowpea chlorotic mottle virus, its protein and nucleic acid. Virology 34:224–239
    [Google Scholar]
  2. Bancroft J. B., Mclean G. D., Rees M. W., Short M. N. 1971; The effect of an arginyl to a cysteinyl replacement on the uncoating behaviour of a spherical plant virus. Virology 45:707–715
    [Google Scholar]
  3. Bancroft J. B., Rees M. W., Dawson J. R. O., Mclean G. D., Short M. N. 1972; Some properties of a temperature-sensitive mutant of cowpea chlorotic mottle virus. Journal of General Virology 16:69–81
    [Google Scholar]
  4. Bancroft J. B., Rees M. W., Johnson M. W., Dawson J. R. O. 1973; A salt-stable mutant of cowpea chlorotic mottle virus. Journal of General Virology 21:507–513
    [Google Scholar]
  5. Boatman S., Kaper J. M. 1972; Forces responsible for the generation of virus structure: The use of SDS to probe protein–RNA interactions. Proceedings of the 1st John Innes Symposium123–134
    [Google Scholar]
  6. Habili N., Francki R. I. B. 1974; Comparative studies on tomato aspermy and cucumber mosaic virus II. Virus stability. Virology 60:29–36
    [Google Scholar]
  7. HollingS M., Stone O. M. 1970; Carnation ringspot virus. Commonwealth Mycological Institute I Association of Applied Biologists. Descriptions of Plant Viruses 21:3 pp
    [Google Scholar]
  8. Horne R. W. 1967; Electron microscopy of isolated virus particles and their components. In Methods in Virology vol III p 521 Edited by Maramorosch K., Koprowski H. New York and London: Academic Press;
    [Google Scholar]
  9. Kalmakoff J., Tremaine J. H. 1967; Some physical and chemical properties of carnation ringspot virus. Virology 33:10–16
    [Google Scholar]
  10. Kaper J. M. 1972; Experimental analysis of the stabilizing interactions of simple RNA viruses: a systematic approach. In RNA Viruses: Replication and Structure, FEES Symposium vol 27 pp 19–41 Edited by Jaspars E. M. J., van Kammen A. Amsterdam: North Holland Publishing Co;
    [Google Scholar]
  11. Kaper J. M. 1973; Arrangement and identification of simple isometric viruses according to their dominating stabilizing interactions. Virology 55:299–304
    [Google Scholar]
  12. Loening U. E. 1969; The determination of molecular weight of ribonucleic acid by polyacrylamide gel electrophoresis. The effect of changes in conformation. Biochemical Journal 113:131–138
    [Google Scholar]
  13. Markham R. 1962; The analytical ultracentrifuge as a tool for the investigation of plant viruses. Advances in Virus Research 9:241–270
    [Google Scholar]
  14. Nelson M. R., Tremaine J. H. 1975; Physiochemical and serological properties of a virus from Saguaro cactus. Virology 65:309–319
    [Google Scholar]
  15. Reichmann M. E. 1965; Determination of ribonucleic acid content of spherical viruses from sedimentation coefficients of full and empty particles. Virology 25:166–169
    [Google Scholar]
  16. Schachman H. K. 1957; Ultracentrifugation, diffusion, and viscometry. In Methods in Enzymology vol IV p 57 Edited by Colowick S. P., Kaplan N. O. New York: Academic Press;
    [Google Scholar]
  17. Tremaine J. H., Ronald W. P., Valcic A. 1975; Aggregation properties of carnation ringspot virus. Phytopathology (in the press)
    [Google Scholar]
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