1887

Abstract

SUMMARY

Organotypic cultures of newborn hamster choroid plexus were inoculated with equal titre doses of newly isolated or hamster adapted strains of mumps virus. The ultrastructure of virus replication in choroid epithelial cells of the cultures was compared. No qualitative differences were observed; however, the adapted strain produced significantly greater numbers of virions and earlier destruction of the cultures. These findings are consistent with previous observations of the ultrastructure of the replication of these strains in the newborn hamster central nervous system. This study lends further support to the hypothesis that differences in the biological effects of the virus strains are primarily the result of virus-cell rather than virus-host interactions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-2-197
1976-02-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/30/2/JV0300020197.html?itemId=/content/journal/jgv/10.1099/0022-1317-30-2-197&mimeType=html&fmt=ahah

References

  1. Azimi P. H., Cramblett H. O., Haynes R. E. 1969; Mumps meningoencephalitis in children. Journal of the American Medical Association 307:509–512
    [Google Scholar]
  2. Bang H. O., Bang J. 1943; Involvement of the central nervous system in mumps. Acta Medica Scandinavica 113:487–505
    [Google Scholar]
  3. Bornstein M. B. 1958; Reconstituted rat-tail collagen used as a substrate for tissue culture on coverslips in maximow slides and roller tubes. Laboratory Investigation 7:134–140
    [Google Scholar]
  4. Bornstein M. B., Murray M. R. 1958; Serial observations on patterns of growth, myelin formation, maintenance and degeneration in cultures of newborn rat and kitten cerebellum. Journal of Biophysical and Biochemical Cytology 4:499–504
    [Google Scholar]
  5. Ennis F. A., Hopps H. E., Douglas R. D., Meyer H. M. 1969; Hydrocephalus in hamsters: induction by natural and attenuated mumps viruses. Journal of Infectious Diseases 119:75–79
    [Google Scholar]
  6. Harter D. H., Choppin P. W. 1971 In Immunological Disorders of the Nervous System pp. 342–355 Edited by Rowland L. P. Baltimore: Williams and Wilkins;
    [Google Scholar]
  7. Herndon R. M., Johnson R. T., Davis L. E., Descalzi L. R. 1974; Ependymitis in mumps virus meningitis: electron microscopical studies of cerebrospinal fluid. Archives of Neurology 30:475–479
    [Google Scholar]
  8. Hoorn B., Tyrell D. A. 1969; Organ cultures in virology. Progress in Medical Virology 11:408–450
    [Google Scholar]
  9. Johnson R. T. 1968; Mumps virus encephalitis in the hamster: studies of the inflammatory response and noncytopathic infection of neurons. Journal of Neuropathology and Experimental Neurology 27:80–95
    [Google Scholar]
  10. Johnson R. T., Johnson K. P. 1968a; Hydrocephalus following viral infection: the pathology of aqueductal stenosis developing after experimental mumps viral infection. Journal Neuropathology and Experimental Neurology 27:591–606
    [Google Scholar]
  11. Johnson R. T., Johnson K. P. 1968b; Myxoviruses and infections of the nervous system. Neurology 18:101–103
    [Google Scholar]
  12. Karnofsky M. J. 1965; A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. Journal of Cell Biology 27:137A
    [Google Scholar]
  13. Kilham L., Margolis G. 1974; Intrauterine infections induced by mumps virus in hamsters. Laboratory Investigations 31:34–41
    [Google Scholar]
  14. Kilham L., Overman J. R. 1953; Natural pathogenicity of mumps virus for suckling hamsters on intracerebral inoculation. Journal of Immunology 70:147–151
    [Google Scholar]
  15. Mainland D. 1963 In Elementary Medical Statistics pp. 218–240 Philadelphia: W. B. Saunders Co;
    [Google Scholar]
  16. Margolis G., Kilham L., Baringer J. R. 1974; A new look at mumps encephalitis: inclusion bodies and cytopathic effects. Journal of Neuropathology and Experimental Neurology 53:13–28
    [Google Scholar]
  17. MountCastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. 1970; Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 6:677–684
    [Google Scholar]
  18. MountCastle W. E., Compans R. W., Lackland H., Choppin P. W. 1974; Proteolytic cleavage of subunits of the nucleocapsid of the paramyxovirus simian virus 5. Journal of Virology 14:1253–1261
    [Google Scholar]
  19. Norrby E., Link H., Olsson J., Panelius M., Salmi A., Vandvik B. 1974; Comparison of antibodies against different viruses in cerebrospinal fluid and serum samples from patients with multiple sclerosis. Infection and Immunity 10:688–694
    [Google Scholar]
  20. Overman J. R., Peers J. H., Kilham L. 1953; Pathology of mumps virus meningoencephalitis in mice and hamster. Archives of Pathology 55:457–465
    [Google Scholar]
  21. Seil F. J., Falk G. A., Kies M. W., Alvord E. C. Jun 1968; The in vitro demyelinating activity of sera from guinea pigs sensitized with whole CNS and purified encephalitogen. Experimental Neurology 22:545–555
    [Google Scholar]
  22. Seil F. J., Herndon R. M. 1970; Cerebellar granule cells in vitro. A light and electron microscopic study. Journal of Cell Biology 45:212–220
    [Google Scholar]
  23. Timmons G. D., Johnson K. P. 1970; Aqueductal stenosis and hydrocephalus after mumps encephalitis. New England Journal of Medicine 283:1505–1507
    [Google Scholar]
  24. Wolinsky J. S., Baringer J. R., Margolis G., Kilham L. 1974; Ultrastructure of mumps virus replication in newborn hamster central nervous system. Laboratory Investigation 31:403–412
    [Google Scholar]
  25. Wolinsky J. S., Gilden D. H., Rorke L. B. 1975a; Experimental panencephalitis induced in suckling mice by parainfluenza I (6/94) virus. III. Ultrastructural studies. Journal of Neuropathology and Experimental Neurology (in the press)
    [Google Scholar]
  26. Wolinsky J. S., Klassen T., Barringer J. R. 1975b; Persistence of neuroadapted mumps virus in newborn hamster brain after intraperitoneal inoculation. Journal of Infectious Diseases (in the press)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-30-2-197
Loading
/content/journal/jgv/10.1099/0022-1317-30-2-197
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error