Canaline, a pyridoxal phosphate antagonist, is shown to inhibit two distinct events in the replication of vaccinia virus in HeLa cells. Initial events proceed in the presence of the inhibitor leading to the formation of DNA-containing, cytoplasmic inclusions. However, further DNA synthesis is required for the subsequent production of infectious progeny following the reversal of canaline inhibition by pyridoxal phosphate. Inhibition of a separate, maturation event is shown by the delayed addition of canaline resulting in the failure to coat virus-specific DNA synthesized previously in the absence of the inhibitor. Thus, the replication of vaccinia virus is sensitive to inhibition by canaline at an early and a late stage in the replication cycle. Reversal is accomplished alternatively by the addition of ‘nonessential’ amino acids suggesting that the effects of canaline result from inhibition of specific protein functions.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error