1887

Abstract

SUMMARY

Canaline, a pyridoxal phosphate antagonist, is shown to inhibit two distinct events in the replication of vaccinia virus in HeLa cells. Initial events proceed in the presence of the inhibitor leading to the formation of DNA-containing, cytoplasmic inclusions. However, further DNA synthesis is required for the subsequent production of infectious progeny following the reversal of canaline inhibition by pyridoxal phosphate. Inhibition of a separate, maturation event is shown by the delayed addition of canaline resulting in the failure to coat virus-specific DNA synthesized previously in the absence of the inhibitor. Thus, the replication of vaccinia virus is sensitive to inhibition by canaline at an early and a late stage in the replication cycle. Reversal is accomplished alternatively by the addition of ‘nonessential’ amino acids suggesting that the effects of canaline result from inhibition of specific protein functions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-1-81
1976-01-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/30/1/JV0300010081.html?itemId=/content/journal/jgv/10.1099/0022-1317-30-1-81&mimeType=html&fmt=ahah

References

  1. Archard L. C., Williamson J. D. 1971; The effect of arginine deprivation on the replication of vaccinia virus. Journal of General Virology 12:249–258
    [Google Scholar]
  2. Archard L. C., Williamson J. D. 1974; Inhibition of vaccinia virus replication by canavanine and canaline. Journal of General Virology 24:493–501
    [Google Scholar]
  3. Bedson H. S., Cruikshank J. O. 1968; Multiple protein functions in the replication of pox virus DNA. Journal of General Virology 3:147–151
    [Google Scholar]
  4. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science, New York 130:432–437
    [Google Scholar]
  5. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18
    [Google Scholar]
  6. Joklik W. K. 1964; The intracellular uncoating of poxvirus DNA I. The fate of radioactively-labelled rabbitpox virus. Journal of Molecular Biology 8:263–276
    [Google Scholar]
  7. Joklik W. K., Becker Y. 1964; The replication and coating of vaccinia DNA. Journal of Molecular Biology 10:452–474
    [Google Scholar]
  8. Kates I. R., McAuslan B. R. 1967; Relationship between protein synthesis and viral deoxyribonucleic acid synthesis. Journal of Virology 1:110–114
    [Google Scholar]
  9. Loh P. C., Riggs J. L. 1961; Demonstration of the sequential development of vaccinia antigens and virus in infected cells: observations with cytochemical and differential fluorescent procedures. Journal of Experimental Medicine 114:149–160
    [Google Scholar]
  10. Rahiala E.-L. 1973; Canaiine: characterization of enzyme-pyridoxal phosphate complex. Acta Chemica Scandinavica 27:3861–3867
    [Google Scholar]
  11. Williamson J. D., Archard L. C. 1974; Preparation and some physico-chemical properties of L-canaline. Life Sciences 14:2481–2490
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-30-1-81
Loading
/content/journal/jgv/10.1099/0022-1317-30-1-81
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error