1887

Abstract

SUMMARY

Viral polypeptides associated with the membrane-free replication complex of poliovirus RNA were multiple in nature. The structural protein precursors [VP0, VP1, VP3] predominated, and because they were found in a cytoplasmic component with the same S value and density as the replication complex are likely to be attached to it . They were not present in the form of empty capsids. The electrophoretic polypeptide pattern of the membrane-bound replication complex was similar but showed a predominance of NCVPX or VP1, unless the cells were slightly depleted in amino acids when the non-structural polypeptide NCVP2 became important. Cystine was the only amino acid capable of reversing this depletion effect on its own.

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1976-01-01
2022-01-21
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References

  1. Abraham G., Cooper P. D. 1975a; Poliovirus polypeptides examined in more detail. Journal of General Virology (in the press)
    [Google Scholar]
  2. Abraham G., Cooper P. D. 1975b; Relations between poliovirus polypeptides as shown by tryptic peptide analysis. Journal of General Virology (in the press)
    [Google Scholar]
  3. Baltimore D. 1968; Inhibition of poliovirus replication by guanidine. In Medical and Applied Virology pp. 340–347 Edited by Sanders M., Lennette E. H. St Louis, Mo.: Warren H. Green Inc;
    [Google Scholar]
  4. Baltimore D., Huang A. S. 1968; Isopycnic separation of subcellular components from poliovirus-infected and normal HeLa cells. Science, New York 162:572–574
    [Google Scholar]
  5. Caliguiri L. A., Compans R. W. 1973; The formation of poliovirus particles in association with the RNA replication complexes. Journal of General Virology 21:99–108
    [Google Scholar]
  6. Caliguiri L. A., Mosser A. G. 1971; Proteins associated with the poliovirus RNA replication complex. Virology 46:375–386
    [Google Scholar]
  7. Caliguiri L. A., Tamm I. 1970a; The role of cytoplasmic membranes in poliovirus biosynthesis. Virology 42:100–111
    [Google Scholar]
  8. Caliguiri L. A., Tamm I. 1970b; Characterisation of poliovirus-specific structures associated with cytoplasmic membranes. Virology 42:112–122
    [Google Scholar]
  9. Cooper P. D., Geissler E., Scotti P. D., Tannock G. A. 1971; Further characterisation of the genetic map of poliovirus temperature-sensitive mutants. In Strategy of the Viral Genome, Ciba Foundation Symposium pp. 75–100 Edited by Wolstenholme G. E. W., Maeve O’Connor. London: Churchill Livingstone;
    [Google Scholar]
  10. Cooper P. D., Stancek D., Summers D. F. 1970; Synthesis of double-stranded RNA by poliovirus temperature-sensitive mutants. Virology 40:971–977
    [Google Scholar]
  11. Cooper P. D., Steiner-Pryor A., Wright P. J. 1973; A proposed regulator for poliovirus: the equestron. Intervirology 1:1–10
    [Google Scholar]
  12. Garwes D. J., Wright P. J., Cooper P. D. 1975; Poliovirus temperature-sensitive mutants defective in cytopathic effects are also defective in synthesis of double-stranded RNA. Journal of General Virology 27:45–59
    [Google Scholar]
  13. Girard M., Baltimore D., Darnell J. E. 1967; The poliovirus replication complex: site for synthesis of poliovirus RNA. Journal of Molecular Biology 24:59–74
    [Google Scholar]
  14. Huang A. S., Baltimore D. 1970; Initiation of polyribosome formation in poliovirus-infected HeLa cells. Journal of Molecular Biology 47:275–291
    [Google Scholar]
  15. Jacobson M. J., Asso J., Baltimore D. 1970; Further evidence on the formation of poliovirus proteins. Journal of Molecular Biology 49:657–669
    [Google Scholar]
  16. Latham H., Darnell J. E. 1965; Entrance of mRNA into HeLa cell cytoplasm in puromycin-treated cells. Journal of Molecular Biology 14:13–22
    [Google Scholar]
  17. Lundquist R. E., Ehrenfeld E., Maizel J. V. 1974; Isolation of a viral polypeptide associated with poliovirus RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 71:4773–4777
    [Google Scholar]
  18. McCahon D., Cooper P. D. 1970; Identification of poliovirus temperature-sensitive mutants having defects in virus structural protein. Journal of General Virology 6:51–62
    [Google Scholar]
  19. Pohjanpelto P. 1958; Stabilisation of poliovirus by cystine. Virology 6:472–487
    [Google Scholar]
  20. Steiner-Pryor A., Cooper P. D. 1973; Temperature-sensitive poliovirus mutants defective in repression of host protein synthesis are also defective in structural protein. Journal of General Virology 21:215–225
    [Google Scholar]
  21. Summers D. F., Maizel J. V. 1971; Determination of the gene sequence of poliovirus with pactamycin. Proceedings of the National Academy of Sciences of the United States of America 68:2852–2856
    [Google Scholar]
  22. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific non-capsid proteins in poliovirus-infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
  23. Taber R., Rekosh D., Baltimore D. 1971; Effect of pactamycin on synthesis of poliovirus proteins: a method for genetic mapping. Journal of Virology 8:395–401
    [Google Scholar]
  24. Wentworth B. B., Mccahon D., Cooper P. D. 1968; Production of infectious RNA and serum-blocking antigen by poliovirus temperature-sensitive mutants. Journal of General Virology 2:297–307
    [Google Scholar]
  25. Wright P. J., Cooper P. D. 1974; Poliovirus proteins associated with ribosomal structures in infected cells. Virology 59:1–20
    [Google Scholar]
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