1887

Abstract

SUMMARY

Plaques were produced in mouse-embryo monolayers by infection with polyoma virus DNA in the presence of diethylaminoethyl-dextran. Optimum conditions for plaque assay were established and dose-response relationships for component I and component (II+III) polyoma DNA determined. Efficiencies up to 6 × 10 p.f.u./g. were obtained for component I DNA.

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1968-12-01
2024-12-12
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References

  1. Bourgaux P., Bourgaux-ramoisy D., Stoker M. 1965; Further studies on transformation by DNA from polyoma virus. Virology 25:364
    [Google Scholar]
  2. Crawford L. V., Crawford E. M., Watson D. H. 1962; The physical characteristics of polyoma virus. I. Two types of particle. Virology 18:170
    [Google Scholar]
  3. Crawford L. V., Dulbecco R., Fried M., Montagnier L., Stoker M. 1964; Cell transformation by different forms of polyoma virus DNA. Proc. natn. Acad. Sci., U.S.A 52:148
    [Google Scholar]
  4. Diamond L., Crawford L. V. 1964; Some characteristics of large-plaque and small-plaque lines of polyoma virus. Virology 22:235
    [Google Scholar]
  5. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis virus. J. exp. Med 99:167
    [Google Scholar]
  6. Dulbecco R., Vogt M. 1963; Evidence for a ring structure of polyoma virus DNA. Proc. natn. Acad. Sci., U.S.A 50:236
    [Google Scholar]
  7. Koch G., Quintrell N., Bishop J. M. 1966; An agar-cell-suspension plaque assay for isolated viral RNA. Biochem. biophys. Res. Commun 24:304
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. molec. Biol 3:208
    [Google Scholar]
  9. Michel M. R., Hirt B., Weil R. 1967; Mouse cellular DNA enclosed in polyoma viral capsids (pseudovirions). Proc. natn. Acad. Sci., U.S.A 58:1381
    [Google Scholar]
  10. Thorne H. V. 1967; Electrophoretic characterization and fractionation of polyoma virus DNA. J. molec. Biol 24:203
    [Google Scholar]
  11. Vaheri A., Pagano J. S. 1965; Infectious poliovirus RNA: a sensitive method of assay. Virology 27:435
    [Google Scholar]
  12. Weil R. 1961; A quantitative assay for a subviral infective agent related to polyoma virus. Virology 14:46
    [Google Scholar]
  13. Weil R., Vinograd J. 1963; The cyclic helix and cyclic coil forms of polyoma viral DNA . Proc. natn. Acad. Sci., U.S.A 50:730
    [Google Scholar]
  14. Winocour E. 1967; On the apparent homology between DNA from polyoma virus and normal mouse synthetic RNA. Virology 3115
    [Google Scholar]
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