1887

Abstract

Serial propagation of varicella virus in tissue culture was accomplished first by Weller (1953) using human embryonic tissue in roller tubes, and later by Taylor-Robinson (1959) in stationary cultures of human fibroblasts and human amnion cells. However, the virus showed a strange anomaly in these systems in that serial transfer of the agent could not be achieved with culture fluids but only by means of intact infected cells. The virus was thus avidly cell-associated and its infectivity and stability appeared to be entirely dependent on cell integrity and viability (Gold, 1965); cell-disruption by any of a variety of procedures resulted in inactivation of the virus. In marked contrast, the virus in human skin vesicles was found to be mostly extracellular since cell-free filtrates of vesicular fluid sometimes had an infective titre almost as high as the unfiltered fluid (Taylor-Robinson, 1959).

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1968-09-01
2022-09-25
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References

  1. Biggs P. M. 1968; Marek’s disease—current state of knowledge. In Current Topics in Microbiology and Immunology Berlin, Heidelberg and New York: Springer;
    [Google Scholar]
  2. Brunell P. A. 1967; Separation of infectious varicella-zoster virus from human embryonic lung fibroblasts. Virology 31:732
    [Google Scholar]
  3. Caunt A. E. 1963; Growth of varicella-zoster virus in human thyroid tissue cultures. Lancet 2:982
    [Google Scholar]
  4. Caunt A. E., Taylor-Robinson D. 1964; Cell-free varicella-zoster virus in tissue culture. J. Hyg., Camb 62:413
    [Google Scholar]
  5. Churchill A. E., Biggs P. M. 1967; Agent of Marek’s disease in tissue culture. Nature, Lond 215:528
    [Google Scholar]
  6. Epstein M. A. 1962; Observations on the mode of release of herpes virus from infected HeLa cells. J. Cell Biol 12:589
    [Google Scholar]
  7. Epstein M. A., Achong B. G. 1965; Fine structural organization of human lymphoblasts of a tissue culture strain (EB 1) from Burkitt’s lymphoma. J. natn. Cancer Inst 34:241
    [Google Scholar]
  8. Epstein M. A., Holt S. J. 1963; Electron microscope observations on the surface adenosine triphosphatase-like enzymes of HeLa cells infected with herpes virus. J. Cell Biol 19:337
    [Google Scholar]
  9. Epstein M. A., Achong B. G., Barr Y. M. 1964; Virus particles in cultured lymphoblasts from Burkitt’s lymphoma. Lancet 1:702
    [Google Scholar]
  10. Epstein M. A., Henle G., Achong B. G., Barr Y. M. 1965; Morphological and biological studies on a virus in cultured lymphoblasts from Burkitt’s lymphoma. J. exp. Med 121:761
    [Google Scholar]
  11. Géder L., Jeney E., Gonczol E. 1964/65; Growth of varicella virus in continuous monkey kidney and human thyroid cell cultures. Acta microbiol. Acad. Sci. Hung 11:361
    [Google Scholar]
  12. Gold E. 1965; Characteristics of herpes zoster and varicella viruses propagated in vitro . J. Immun 95:683
    [Google Scholar]
  13. Lane W. F., Marshall J. 1957; Experience with human amnion cell culture as a routine diagnostic method in a bacteriological laboratory. Mon. Bull. Minist. Hlth. Lab. Serv 16:198
    [Google Scholar]
  14. Melnick J. L., Midulla M., Wimberly I., Barrera-Oro J. G., Levy B. M. 1964; A new member of the herpes virus group isolated from South American marmosets. J. Immun 92:596
    [Google Scholar]
  15. Taylor-Robinson D. 1959; Chickenpox and herpes zoster. III. Tissue culture studies. Br. J. exp. Path 40:521
    [Google Scholar]
  16. Weller T. H. 1953; Serial propagation in vitro of agents producing inclusion bodies derived from varicella and herpes zoster. Proc. Soc. exp. Biol. Med 83340
    [Google Scholar]
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