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Biochemical Studies on Influenza Virus Multiplication at Reduced Temperatures
Abstract
Summary
At 25° all components of fowl plague virus were synthesized in chick embryo fibroblasts. The rate of formation was about a quarter of that at 37°, but the final yield was the same. Infectious virus, however, was not formed at 25°. If infected cells were preincubated at 25° for 20 hr and thereafter kept at 37°, a normal yield of infectious progeny was found. With longer preincubation at 25° a decreasing yield of infectious virus was found. This effect may have been due to the synthesis at 25° of a viral component responsible for the cytopathic effect which acts only at the higher temperature. Preincubation of infected cells at 37° for different times and further incubation at 25° revealed a virus-specific precursor synthesized early in the infectious cycle which was rate limiting at 25°.
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© Journal of General Virology 1968