1887

Abstract

Summary

At 25° all components of fowl plague virus were synthesized in chick embryo fibroblasts. The rate of formation was about a quarter of that at 37°, but the final yield was the same. Infectious virus, however, was not formed at 25°. If infected cells were preincubated at 25° for 20 hr and thereafter kept at 37°, a normal yield of infectious progeny was found. With longer preincubation at 25° a decreasing yield of infectious virus was found. This effect may have been due to the synthesis at 25° of a viral component responsible for the cytopathic effect which acts only at the higher temperature. Preincubation of infected cells at 37° for different times and further incubation at 25° revealed a virus-specific precursor synthesized early in the infectious cycle which was rate limiting at 25°.

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/content/journal/jgv/10.1099/0022-1317-3-2-239
1968-09-01
2022-01-24
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References

  1. Davenport F. M., Rott R., Schäfer W. 1960; Physical and biological properties of influenza virus components obtained after ether treatment. J. exp. Med 112:765
    [Google Scholar]
  2. Drzeniek R. 1967; Differences in splitting capacity of virus and V. cholerae neuraminidase on sialic acid substrates. Biochem. biophys. Res. Commutt 26:631
    [Google Scholar]
  3. Fulton F., Dumbell K. R. 1949; The serological comparison of strains of influenza virus. J. gen. Microbiol 3:97
    [Google Scholar]
  4. Hennessen W. 1955; Über eine Influenza-Komplementbindungsreaktion für die Praxis. Z. Hyg. Infektkrankh 141:557
    [Google Scholar]
  5. Rott R., Scholtissek C. 1967; Multiplication of myxoviruses. In Modern Trends in Medical Virology p 25 Ed Heath R. B., Waterson A. P. Butterworths: London;
    [Google Scholar]
  6. Schäfer W., Zimmerman T., Schuster H. 1959; Inaktivierung verschiedener menschenund tierpathogener Virusarten sowie des Tabakmosaikvirus durch salpetrige Saure. Z. Naturf 14b:643
    [Google Scholar]
  7. Scholtissek C. 1968; Nucleotide metabolism in tissue culture cells at low temperatures. III. Studies in vitro . Biochim. biophys. Acta 155:14
    [Google Scholar]
  8. Scholtissek C., Rott R. 1961; Zusammenhänge zwischen der Synthese von Ribonukleinsäure und Protein bei der Vermehrung eines Virusder Influenzagruppe (Virus der klassischen Geflügel-pest). Z. Naturf 16b:663
    [Google Scholar]
  9. Scholtissek C., Becht H., Drzeniek R. 1967; Biochemical studies on the cytopathic effect of influenza virus. J. gen. Virol 1:219
    [Google Scholar]
  10. Zimmermann T., Schäfer W. 1960; Effect of p-fluorophenylalanine on fowl plague virus multiplication. Virology 11:676
    [Google Scholar]
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