1887

Abstract

Summary

Larvae of the small tortoiseshell butterfly, (Lepidoptera: Nymphalidae), were infected orally with a nuclear polyhedrosis virus. Sections of larvae which had been killed and fixed at intervals after infection were examined by light and electron microscopy. Typical virus development was observed in the fat body cells. However, in the nuclei of infected columnar cells of the mid-gut no incorporation of the virus particles into the polyhedral inclusion body was observed although both virus particles and crystalline polyhedron protein were present. Subsequently virus particles were found in the cytoplasm of these cells between the nucleus and the basal membrane. It is proposed that this unusual virus development is significant in the pathway of infection of a nuclear polyhedrosis virus.

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/content/journal/jgv/10.1099/0022-1317-3-2-221
1968-09-01
2024-04-26
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References

  1. Anderson E., Harvey W. R. 1966; Active transport by the Cecropia mid-gut. II. Fine structure of the mid-gut epithelium. J. Cell Biol 31:107
    [Google Scholar]
  2. Bergold G. H. 1958; Viruses of insects. In Handbuch der Virusforschung vol iv pp 60–142 Ed Hallauer C, Meyer K. F. Vienna: Springer;
    [Google Scholar]
  3. Bird F. T. 1964; On the development of insect polyhedrosis and granulosis virus particles. Can. J. Microbiol 10:49
    [Google Scholar]
  4. Harrap K. A., Juniper B. E. 1966; The internal structure of an insect virus. Virology 29:175
    [Google Scholar]
  5. Krywienczyk J. 1963; Demonstration of nuclear polyhedrosis in Bombyx mori by fluorescent antibody technique. J. Insect Path 5:309
    [Google Scholar]
  6. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol 17:208
    [Google Scholar]
  7. Smith K. M., Xeros N. 1953; Development of virus in the cell nucleus. Nature, Lond 172:670
    [Google Scholar]
  8. Steinhaus E. A. 1960; The duration of viability and infectivity of certain insect pathogens. J. Insect Path 2:225
    [Google Scholar]
  9. Trager W. 1935; Cultivation of the virus of grasserie in silkworm tissue cultures. J. exp. Med 61:501
    [Google Scholar]
  10. Xeros N. 1953; Origin and fate of the virus bundles in nuclear polyhedroses. Nature, Lond 172:548
    [Google Scholar]
  11. Xeros N. 1955; Origin of the virus producing chromatic mass or net of the insect nuclear polyhedroses. Nature, Lond 175:588
    [Google Scholar]
  12. Xeros N. 1956; The virogenic stroma in nuclear and cytoplasmic polyhedroses. Nature, Lond 178:412
    [Google Scholar]
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