1887

Abstract

Summary

The haemagglutinating ability of three strains of IBV was investigated. It was shown that whereas strain Beaudette had no detectable haemagglutinin, both Connecticut and Massachusetts agglutinated red cells of various species. The haemagglutinin of Connecticut was detectable after sucrose gradient purification whereas that of Massachusetts required both the purification step and incubation with the enzyme phospholipase C to reveal it. The agglutination could be inhibited by specific antisera. Some studies on the nature of the red cell receptor, and the possible presence of a receptor destroying enzyme, are reported.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-28-3-381
1975-09-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/28/3/JV0280030381.html?itemId=/content/journal/jgv/10.1099/0022-1317-28-3-381&mimeType=html&fmt=ahah

References

  1. Bingham R. W. 1975; The polypeptide composition of avian infectious bronchitis virus. (in the press)
    [Google Scholar]
  2. Biswal N., Nazerian K., Cunningham C. H. 1966; A haemagglutinating fraction of infectious bronchitis virus. American Journal of Veterinary Research 27:1157–1167
    [Google Scholar]
  3. Bradburne A. F. 1970; Antigenic relationships amongst coronaviruses. Archiv für die gesamte Virusfor-schung 31:352–364
    [Google Scholar]
  4. Buckland F. E., Tyrrell D. A. J. 1963; A comparative study of virus haemagglutinins. The stability of haemagglutinins and red cell receptors to certain physical and chemical treatments. Journal of General Microbiology 32:241–253
    [Google Scholar]
  5. Clarke D. H., Casals J. 1958; Techniques for haemagglutination and haemagglutination inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  6. Compans R. W., Klenk H. D., Caliguiri L. A., Choppin P. W. 1970; Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycopeptides. Virology 42:880–889
    [Google Scholar]
  7. Corbo L. J., Cunningham C. H. 1959; Haemagglutination by trypsin-modified infectious bronchitis virus. American Journal of Veterinary Research 20:876–883
    [Google Scholar]
  8. Diner B. A. 1970; Purification and properties of Clostridium welchii phospholipase C. Biochimica et Bio-physica Acta 198:514–522
    [Google Scholar]
  9. Hierholzer J. C., Palmer E. L., Whitfield S. G., Kaye H. S., Dowdle W. R. 1972; Protein composition of coronavirus OC43. Virology 48:516–527
    [Google Scholar]
  10. Kaye H. S., Dowdle W. R. 1969; Some characteristics of haemagglutination of certain strains of ‘IBV-like’ virus. The Journal of Infectious Diseases 120:576–581
    [Google Scholar]
  11. Kendal A. P. 1974; A new receptor destroying enzyme in influenza C?. Behring Institute Mitteilungen 55:18–31
    [Google Scholar]
  12. Mcintosh K. 1974; Coronaviruses; a comparative review. Current Topics in Microbiology and Immunology 63:85–129
    [Google Scholar]
  13. Schäfer W., Szántó J. 1969; Studies on mouse leukaemia viruses. II. Nachweis eines virusspezifischen Hamagglutinins. Zeitschrift für Naturforschung B24:1324–1331
    [Google Scholar]
  14. Sørensen S. P. L. 1912; Uber die Messung und Bedeutung der Wasserstoffionenkonzentration bei bio-logischen Prozessen. Ergebnisse der Physiologie 12:393–532
    [Google Scholar]
  15. Szántó J. 1971; Haemagglutination reaction with avian myeloblastosis virus. Acta Virologica Prague 15:245–248
    [Google Scholar]
  16. Tyrrell D. A. J., Almeida J. D., Berry D. M., Cunningham C. H., Hamre D., Hofstad M. S., Mallucci L., Mcintosh K. 1968; Coronaviruses. Nature, London 220:650
    [Google Scholar]
  17. Witter R., Frank H., Moennig V., Hunsmann G., Lange J., Schäfer W. 1973; Properties of mouse leukaemia viruses. IV. Haemagglutination assay and characterisation of haemagglutinating surface components. Virology 54:330–345
    [Google Scholar]
  18. Zwaal R. F. A., Roelofsen B., Comfurius P., van Deenen L. L. M. 1971; Complete purification and some properties of phospholipase C from Bacillus cereus. Biochimica et Biophysica Acta 233:474–479
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-28-3-381
Loading
/content/journal/jgv/10.1099/0022-1317-28-3-381
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error