1887

Abstract

Summary

The SV40 DNA that was generated by undiluted passaging of the virus was analysed by agarose gel electrophoresis. Nine bands of virus DNA were distinguished and each band contained a specific size class of DNA, all shorter than the complete SV40 genome as was determined by electron microscopy measurements. A difference of 2% in length, about 100 base pairs, resulted in a clear band splitting. Two sets of undiluted passaging were established and the defective DNA in the two sets had both different and similar size classes varying in length from 96% to 73% of the unit length SV40 DNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-28-3-285
1975-09-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/28/3/JV0280030285.html?itemId=/content/journal/jgv/10.1099/0022-1317-28-3-285&mimeType=html&fmt=ahah

References

  1. Aay C., Borst P. 1972; The gel electrophoresis of DNA. Biochimica et Biophysica Acta 269:192–200
    [Google Scholar]
  2. Brockman W. W., Nathans D. 1974; The isolation of simian virus 40 variants with specifically altered genomes. Proceedings of the National Academy of Sciences of the United Slates of America 71:942–946
    [Google Scholar]
  3. Davis R. W., Simon M., Davidson N. 1971 Methods in Enzymology 21:413–428
    [Google Scholar]
  4. Espejo R. T., Canelo E. S. 1968; Properties of bacteriophage PM2: a lipid-containing bacterial virus. Virology 34:738–747
    [Google Scholar]
  5. Fried M. 1974; Isolation and partial characterization of different defective DNA molecules derived from polyoma virus. Journal of Virology 13:939–946
    [Google Scholar]
  6. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  7. Huang A. S. 1973; Defective interfering viruses. Annual Review of Microbiology 27:101–117
    [Google Scholar]
  8. Jansz H. S., Pouwels P. H. 1965; Structure of the replicative form of bacteriophage 174. Biochemical and Biophysical Research Communications 18:589–594
    [Google Scholar]
  9. Jansz H. S., Pouwels P. H., Schiphorst S. 1966; Preparation of double-stranded DNA (replicative form) of bacteriophage (ɸ X 174: a simplified method. Biochimica et Biophysica Acta 123:626–627
    [Google Scholar]
  10. Mccutchan I. H., Pagano R. S. 1968; Enhancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran. Journal of the National Cancer Institute 41:351–357
    [Google Scholar]
  11. Mertz I. E., Berg P. 1974; Defective simian virus 40 genomes: isolation and growth of individual clones. Virology 62:112–124
    [Google Scholar]
  12. Pettersson U., Mulder C., Delius H., Sharp P. A. 1973; Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R.RI. Proceedings of the National Academy of Sciences of the United States of America 70:200–204
    [Google Scholar]
  13. Tai H. T., Smith C. A., Sharp P. A., Vinograd I. 1972; Sequence heterogeneity in closed simian virus 40 deoxyribonucleic acid. Journal of Virology 9:317–325
    [Google Scholar]
  14. Uchida S., Watanabe S., Kato M. 1966; Incomplete growth of simian virus 40 in African green monkey kidney culture induced by serial undiluted passages. Virology 28:135–141
    [Google Scholar]
  15. Uchida S., Yoshiike K., Watanabe S., Furuno A. 1968; Antigen forming defective viruses of simian virus 40. Virology 34:1–8
    [Google Scholar]
  16. Yoshiike K. 1968; Studies on DNA from low-density particles of SV40. II. Non-infectious virions associated with a large-plaque variant. Virology 34:402–409
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-28-3-285
Loading
/content/journal/jgv/10.1099/0022-1317-28-3-285
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error