1887

Abstract

SUMMARY

Evidence is presented that adaptation of IPN virus (strain VR 299) to FHM cells entails the selection of a variant virus type that differs significantly from the parental, and most representative, RTG-2 virus type in being able to adsorb efficiently to, and form plaques in, FHM cells.

The plaque titre of FHM-non-adapted virus stocks (RTG-2 viruses) was reduced by at least 99.99% in FHM cells, while FHM-adapted virus stocks (FHM viruses) produced plaques at equally high titres in both RTG-2 and FHM cells.

FHM viruses and RTG-2 viruses differed also in their behaviour in RTG-2 cells in respect to plaque size distribution and growth characteristics, but both virus-types were shown to be morphologically identical, and no significant difference in reactivity against specific antiserum could be detected.

Analysis of virus in individual RTG-2 plaque isolates or plaque progeny shows that a mutation of relatively high frequency (10 to 10) probably causes the ability to infect the FHM cells efficiently. Only these mutant virus-types were found in FHM plaque isolates.

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1975-07-01
2022-08-18
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References

  1. Ball H. I., Munro A. L., Ellis A., Elson K. G., Hodgkiss W., McFarlane I. S. 1971; Infectious pancreatic necrosis in Rainbow Trout in Scotland. Nature, London 234:417
    [Google Scholar]
  2. Breeze D. C., Thorne H. V. 1966; Electrophoretic separation of two plaque type variants of encephalomyocarditis virus. Nature, London 210:1080
    [Google Scholar]
  3. Choppin P. W. 1963; Multiplication of two kinds of influenza A2 virus particles in monkey kidney cells. Virology 21:342–352
    [Google Scholar]
  4. Cohen J., Poinsard A., Scherrer R. 1973; Physico-chemical and morphological features of infectious pancreatic necrosis virus. Journal of General Virology 21:485–498
    [Google Scholar]
  5. Cohen J., Scherrer R. 1972; Structure de la capside du virus de la Nécrose Pancréatique Infectieuse (NPI). Compte rendu hebdomadaire des séances de I’Academie des Sciences, Paris 274:1222–1225
    [Google Scholar]
  6. Clem L. W., Sigel M. M., FRiis R. R. 1965; An orphan virus isolated in marine fish cell tissue culture. Annals of the New York Academy of Sciences 126:343–361
    [Google Scholar]
  7. Colter J. S., Davies M. A., Campbell J. B. 1964; Studies of three variants of Mengo encephalomyelitis virus. I. Rate of attachment to L cells and effects of pH on infectivity. Virology 24:474–480
    [Google Scholar]
  8. De Kinkelin P., Dorson M. 1973; Interferon production in Rainbow Trout (Salmo gairdneri) experimentally infected with Egtved virus. Journal of General Virology 19:125–127
    [Google Scholar]
  9. Diamond L., Crawford L. V. 1964; Some characteristics of large and small plaque lines of polyoma virus. Virology 22:235–244
    [Google Scholar]
  10. Gravell M., Malsberger R. G. 1965; A permanent cell line from the fathead minnow (Pimephales promelas). Annals of the New York Academy of Sciences 126:555–565
    [Google Scholar]
  11. Holland J. J. 1961; Receptor affinities as major determinants of enterovirus tissue tropisms in humans. Virology 15:312–326
    [Google Scholar]
  12. Holland J. J., mclaren L. C., syverton J. T. 1959; The mammalian cell-virus relationship. IV. Infections of naturally insusceptible cells with enterovirus nucleic acid. Journal of Experimental Medicine no:65–80
    [Google Scholar]
  13. Kelly R. K., Loh P. C. 1972; Electron microscopical and biochemical characterization of infectious pancreatic necrosis virus. Journal of Virology 10:824–834
    [Google Scholar]
  14. Lowry S. P., Melnick J. L., Rawls W. E. 1971; Investigation of plaque formation in chick embryo cells as a biological marker for distinguishing herpes virus type 2 from type 1. Journal of General Virology 10:1–9
    [Google Scholar]
  15. Malsberger R. G., Cerini C. P. 1963; Characteristics of infectious pancreatic necrosis virus. Journal of Bacteriology 86:1283–1287
    [Google Scholar]
  16. McLaren L. C., Holland I. J., Syverton J. T. 1959; The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non primate origin. Journal of Experimental Medicine 109:475–485
    [Google Scholar]
  17. Moss L. H., Gravell M. 1969; Ultrastructure and sequential development of infectious pancreatic necrosis virus. Journal of Virology 3:52–58
    [Google Scholar]
  18. Ozaki Y., Kumagai K. 1972; Effects of polyions on Japanese encephalitis virus. Difference in interaction of virus with DEAE-dextran and dextran sulfate between PS-adapted and non-adapted virus. Archiv fur die Gesamte Virusforschung 39:83–91
    [Google Scholar]
  19. Roizman B., Roane P. R. Jun 1963; Demonstration of a surface difference between virions of two strains of herpes simplex virus. Virology 19:198–204
    [Google Scholar]
  20. Scherrer R., Bic E., Cohen J. 1974; Le virus de la Necrose Pancreatique Infectieuse (NPI): etude de la replication et de l’induction de la synthese d’interferon en fonction de l’hote et de la temperature. Annales de Microbiologie (Institut Pasteur) 125 A:455–467
    [Google Scholar]
  21. Scherrer R., Cohen J. 1971 In Proceedings of the 2nd International Congress of Virology p 263 Edited by Melnick J. L. Basel: Karger;
    [Google Scholar]
  22. Thorne H. V., House W., Kish A. L. 1965; Electrophoretic properties and purification of large and small plaque forming strains of polyoma virus. Virology 27:37–43
    [Google Scholar]
  23. Vestergard-Jorgensen P., Grauballe P. C. 1971; Problems in the serological typing of IPN virus. Acta Veterinaria Scandinavica 12:145–147
    [Google Scholar]
  24. Vestergard-jorgensen P., Kehlet N. P. 1971; Infectious pancreatic necrosis (IPN) in Danish rainbow trout. Their serological and pathogenic properties. Nordisk Veterinoermedicin 23:568–575
    [Google Scholar]
  25. Wolf K. 1966; The fish viruses. In Advances in Virus Research vol 12 pp 35–101 Edited by Smith K. M., Lauffer M. A. New York: Academic Press;
    [Google Scholar]
  26. Wolf K., Quimby M. C. 1962; Established eurythermic line of fish cells in vitro. Science, New York 135:1065–1066
    [Google Scholar]
  27. Wolf K., Quimby M. C. 1971; Salmonid viruses: infectious pancreatic necrosis virus. Morphology, pathology and serology of first European isolations. Archiv fur die Gesamte Virusforschung 34:144–156
    [Google Scholar]
  28. Wolf K., Snieszko S. F., Dunbar C. E., Pyle E. 1960; Virus nature of infectious pancreatic necrosis in trout. Proceedings of the Society for Experimental Biology and Medicine 104:105–108
    [Google Scholar]
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