1887

Abstract

SUMMARY

-rhamnose has been found to be useful to investigate the process of irreversible adsorption of PL-1 phage to its host bacterium, ATCC27092. -rhamnose inhibited phage adsorption to cells without inactivating free phages. Adsorption inhibition was correlated with the concentrations of -rhamnose. The inhibitory effect of -rhamnose on phage adsorption was of a competitive nature against host cells. Among other saccharides tested, -fucose, -mannose and -ribose showed a slight degree of adsorption-inhibiting activity.

In early stages of phage adsorption in a tris-maleate buffer, where the binding of phages to cells was still reversible, addition of -rhamnose resulted in the partial desorption of phages from the cells to which they had adsorbed. However, the number of infective phages desorbed by -rhamnose treatment gradually fell off as incubation continued, showing that the phages became firmly bound to the cells. Therefore, it is possible to determine the number of phages irreversibly adsorbed to cells by using this desorption technique with -rhamnose. The process of irreversible phage adsorption, that is, the formation of phage-cell complexes from which no more infective phages could be desorbed, was dependent on temperature and strongly inhibited at 0 °C.

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/content/journal/jgv/10.1099/0022-1317-28-1-29
1975-07-01
2022-01-16
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References

  1. Hershey A. D., Chase M. 1952; Independent functions 0l \iral protein and nucleic acid in growth of bacteriophage. Journal of General Physiology 36:39–56
    [Google Scholar]
  2. Luria S. E., Steiner D. L. 1954; The role of calcium in the penetration of bacteriophage T5 into its host. Journal of Bacteriology 67:635–639
    [Google Scholar]
  3. Seto S., Osawa T., Yamamoto S. 1964; Studies on bacteriophages for l-glutamic acid-producing bacterium, Microbacterium ammoniaphilum. Aminosan-Kakusan 10:27–36
    [Google Scholar]
  4. Yokokura T. 1971; Phage receptor material in Lactobacillus casei cell wall. I. Effect of l-rhamnose on phage adsorption to the cell wall. Japanese Journal of Microbiology 15:457–463
    [Google Scholar]
  5. Watanabe K., Takesue S., Iin-Nai K., Yoshikawa T. 1970; Bacteriophage active against the lactic acid beverage-producing bacterium, Lactobacillus casei. Applied Microbiology 20:409–415
    [Google Scholar]
  6. Watanabe K., Takesue S. 1973; Energy requirement for the formation of blender-resistant complexes in Lactobacillus phage infection. Journal of General Virology 20:319–326
    [Google Scholar]
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