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The best conditions found for infection of tobacco mesophyll protoplasts with alfalfa mosaic virus (AMV) were pH 5.2, 2.5 μg/ml virus and 1 μg/ml poly-l-ornithine. Newly synthesized virus could be detected 15 h after inoculation and the virus concentration reached an estimated 106 to 107 particles per infected protoplast 2 days after inoculation.
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